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Reactive Thin Polymer Films as Platforms for the Immobilization of Biomolecules

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Microfabrication of Stimuli-Responsive Polymers

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Abstract

In this chapter, spin-coated thin films of poly(N-hydroxysuccinimidyl-methacrylate) (PNHSMA) were investigated as reactive layers for obtaining platforms for biomolecule immobilization with high molecular loading. The surface reactivity of PNHSMA films in coupling reactions with amino-functionalized poly(ethylene glycol) (Mn: 500 g/mol) (PEG500-NH2) was determined by FTIR spectroscopy, X-ray photoelectron spectroscopy (XPS), fluorescence microscopy, and ellipsometry measurements, respectively.

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Authors and Affiliations

  1. School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, China

    Chuanliang Feng

  2. School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, China

    Xiaoqiu Dou

  3. School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, China

    Yibin Xu

Authors
  1. Chuanliang Feng
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  2. Xiaoqiu Dou
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  3. Yibin Xu
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Correspondence to Chuanliang Feng .

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Feng, C., Dou, X., Xu, Y. (2021). Reactive Thin Polymer Films as Platforms for the Immobilization of Biomolecules. In: Microfabrication of Stimuli-Responsive Polymers. Springer, Singapore. https://doi.org/10.1007/978-981-33-6869-9_4

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