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Applications of NMR in Cancer Research

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Unravelling Cancer Signaling Pathways: A Multidisciplinary Approach

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Abstract

Nuclear magnetic resonance (NMR) spectroscopy has been widely applied to many facets of cancer research. As the name implies, NMR spectroscopy is a technique that utilizes transitions between different states of a nucleus when placed in a magnetic field. NMR has been used for decades to identify and quantify the reactants and products associated with chemical reactions. More recently, it has been used in the field termed metabolomics to identify and quantify the small molecules of complex mixtures and how that molecular repertoire varies under various perturbations to the system being studied. NMR is also used for de novo three-dimensional structure determination of macromolecules including proteins and protein domains as well as to quantify the motions inherent in structures. A great strength of the technique is to characterize the interactions of small molecules with these macromolecules, which can be used for inhibitor discovery and development. These applications have direct relevance to cancer research and are described in this review.

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Acknowledgments

Funding for this research has been provided in part by the Michael J. Rainen Family Foundation grant (AAH) and grants from the National Institutes of Health, R01-AI38001 and R01-GM067985 (JB) and P50-HL110789 and R01-HL136485 (AK). Acquisition of the NMR spectrometer was assisted by a NIH instrumentation grant (S10OD020073). The authors would like to thank Dr. Janet M. Cowan, Pathology and Laboratory Medicine, Tufts Medical Center, for her invaluable technical assistance and advice in the growth of BCC cultures. We would also like to thank Tatiana Mendez and Betsey Philip for their assistance with NMR data acquisition.

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Authors and Affiliations

  1. Tufts University School of Medicine, Boston, MA, USA

    James D. Baleja & Tiffany Corlin

  2. Center for Hemostasis and Thrombosis Research, Tufts Medical Center, Boston, MA, USA

    Athan Kuliopulos

  3. Tufts University School of Dental Medicine, Boston, MA, USA

    Addy Alt-Holland

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  1. James D. Baleja
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  2. Tiffany Corlin
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  3. Athan Kuliopulos
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  4. Addy Alt-Holland
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Corresponding author

Correspondence to James D. Baleja .

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Editors and Affiliations

  1. Integrated Biophysics and Structural Biology Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai, Maharashtra, India

    Kakoli Bose

  2. Comparative Oncology Program and Small Animal Imaging Facility, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai, Maharashtra, India

    Pradip Chaudhari

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Baleja, J.D., Corlin, T., Kuliopulos, A., Alt-Holland, A. (2019). Applications of NMR in Cancer Research. In: Bose, K., Chaudhari, P. (eds) Unravelling Cancer Signaling Pathways: A Multidisciplinary Approach. Springer, Singapore. https://doi.org/10.1007/978-981-32-9816-3_13

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