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Droplet-Based Microfluidics for Single-Cell Encapsulation and Analysis

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Microfluidics for Single-Cell Analysis

Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

Droplet microfluidic techniques have been rapidly developed as a powerful tool to perform high-throughput and low-cost analysis of single cells. Microscale droplets can be easily produced by a microfluidic manipulation to encapsulate and manipulate single cells for precise analysis. This offers a new approach to measure genetic and functional heterogeneity of cell division, growth, metabolism, and apoptosis. Functional characteristics of cellular molecules, such as DNA , RNA, and proteins, can be realized at a single-cell level. In this chapter, we will present a general introduction to single-cell analysis involving droplet-based microfluidic techniques. We will highlight the current state of droplet-based microfluidic single-cell analysis for deep insights understanding the biological process at the single-cell level.

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  1. Department of Chemistry, Tsinghua University, Beijing, 100084, People’s Republic of China

    Qiushui Chen & Jin-Ming Lin

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  1. Department of Chemistry, Tsinghua University, Beijing, China

    Jin-Ming Lin

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Chen, Q., Lin, JM. (2019). Droplet-Based Microfluidics for Single-Cell Encapsulation and Analysis. In: Lin, JM. (eds) Microfluidics for Single-Cell Analysis. Integrated Analytical Systems. Springer, Singapore. https://doi.org/10.1007/978-981-32-9729-6_5

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