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GC-MS Analysis of Organic Extracts of Cymbidium aloifolium (L.) Sw. (Orchidaceae) Leaves from Eastern Ghats of India

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Orchid Biology: Recent Trends & Challenges

Abstract

Phytochemical constituents of various leaf extracts of Cymbidium aloifolium (L.) Sw. have been studied using gas chromatography–mass spectrometry (GC-MS) data. The dried leaves powder were extracted with methanol at room temperature by using a Soxhlet extractor. The methanol crude extract of Cymbidium aloifolium was again taken with hexane, chloroform and ethyl acetate. The analysis was carried out on an Agilent GC-MS equipped with a HP-5MS fused capillary column; the compounds are separated using helium as a carrier gas at a constant flow of 1 ml/min. A qualitative analysis of various organic extracts showed eight different photochemical compounds, namely n-hexadecanoic acid; 9,12-octadecadienoic acid (Z,Z); 9,12,15-octadecatrienoic acid, (Z,Z,Z); octadecanoic acid; phytol; 2-butyne; 2-cyclopenten-1-one; and 1,4-benzenedicarboxylic acid. Most of the identified compounds are biologically important. This study offers a platform of using Cymbidium aloifolium leaves as herbal alternatives for various diseases. The compounds reported in this investigation also have some phylogenetic significance.

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Acknowledgements

The authors acknowledge Dr. K. Babu, Post-Doctoral Fellow of the Department of Botany & Microbiology, Acharya Nagarjuna University, for his help in carrying out our entire work.

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Rampilla, V., Khasim, S.M. (2020). GC-MS Analysis of Organic Extracts of Cymbidium aloifolium (L.) Sw. (Orchidaceae) Leaves from Eastern Ghats of India. In: Khasim, S., Hegde, S., González-Arnao, M., Thammasiri, K. (eds) Orchid Biology: Recent Trends & Challenges . Springer, Singapore. https://doi.org/10.1007/978-981-32-9456-1_26

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