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Abstract

Seeds are the basic input for crop production. Infected or contaminated seed is the primary source of inoculum for a large number of destructive diseases of important food, fodder and fibre crops. In this context, seed health tests have become a major component towards sustainable crop production and crop productivity. Apart from seed health testing, the seed certification for a crop comprises legal norms to be qualified for ensuring genetic identity, physical purity, germinability and freedom from seed-transmitted pathogens and weeds. Mainly different types of conventional methods followed by molecular methods are employed for seed health testing. Conventional methods include visual inspection of seeds, washing test, seed soak method and incubation methods, while serological assay and nucleic acid-based techniques are the most accurate and least time-consuming which comes under molecular method of seed health testing. The conventional methods are mostly based on interpretation of visual symptoms, culturing and laboratory identification. Thus, it is almost difficult to interpret results with hidden symptoms. However, among all techniques available, nucleic acid-based techniques are the most accurate and least time-consuming.

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References

  • Agarwal VK, Sinclair JB (1997) Seed treatment. Principles of seed pathology. CRC Press Inc/Lewis Publishers, Boca Raton, pp 423–460

    Google Scholar 

  • Agarwal VK, Srivastava AK (1985) NaOH seed soak method for routine examination of rice seed lots for rice bunt. Seed Res 13:159

    Google Scholar 

  • Brinkman A (1931) Thesis, University of Amsterdam, NV Hollandia-Drukkerij, Baarn, p 86

    Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay. J Gen Virol 34:475–483

    Article  CAS  Google Scholar 

  • Dadlani M, Tonapi VA, Prasad R et al (2013) Seed Quality Assurance Book, p 185

    Google Scholar 

  • Dastur JF (1932) Foot rot and black point disease of wheat in central provinces. Agric Live Stk India 3:275–282

    Google Scholar 

  • Doyer LC (1938) International seed testing Association Veenman H, Zonen Wageningen, Holland, p 58

    Google Scholar 

  • Guar YD, Khetrapal RK (1994) Diagnostic tests for seed borne microbes with special emphasis on immunological with special molecular approaches. In: Singh TB, Trivedi PC (eds) Vistas in seed biology, vol II. Printwell Publishers, Jaipur, pp 337–356

    Google Scholar 

  • Halfon-Meiri A (1970) Infection of chickpea seeds by Ascochyta rabiei in Israel. Plant Dis Rep 54(5):442–445

    Google Scholar 

  • Johnson HW, Jones JP (1962) Purple stain of guar. Phytopathology 52(1):269–271

    Google Scholar 

  • Johnson HW, Lefebvre CL (1942) Downy mildew of soybean seeds. Plant Dis Rep 26:49–50

    Google Scholar 

  • Jones FR, Torrie JH (1946) Systemic infection of downy mildew in soybean and Allalfa. Phytopathology 36(12):1057–1059

    Google Scholar 

  • Khandzada AK, Rennie WJ, Mathur SB, Neergaard P (1980) Evaluation of two routine embryo test procedures for assessing the incidence of loose smut infection in seed samples of wheat (Triticum aestivum) (No. 81–645687. CIMMYT)

    Google Scholar 

  • Khetrapal RK (2004) A critical appraisal of seed health certification and transboundary movement of seed under WTO regime. Indian Phytopathol 57(4):408–421

    Google Scholar 

  • Khetrapal RK, Chalam VC (2012) Training manual on seed quality assurance. In: Prasad M, Yadav SK, Arum Kumar MB, Lal SK, Tonapi VA (eds) Division of seed science and technology. IARI, New Delhi, pp 191–200

    Google Scholar 

  • Khetrapal RK, Kumar CA (1995) ELISA methodology. In: Singh RP, Singh US (eds) Molecular methods in plant pathology. CRC Press. Inc, Boca Raton, pp 329–341

    Google Scholar 

  • Kilpatrick K (1957) Fungi associated with the flowers, pods, and seeds of soybeans. Phytopathology 47(3):131–135

    Google Scholar 

  • Kondo M, Okamura T (1927) Untersuchungen der versachiedenen Reiskorner geringerer qualitat. I. Die brarun gefarbten enthulsten Reiskorner. Ischamai Ber Ohara Inst Landw Forsch 3:406–419

    Google Scholar 

  • Kulik MM (1973) Comparison of blotters and guaiacol agar for detection of Helminthosporium oryzae and Trichoconis padwickii in rice seeds. Phytopathology 65:1325–1326. https://doi.org/10.1094/Phyto-65-1325

    Article  Google Scholar 

  • Laviolette FA, Athow KL (1972) Cercospora kikuchii infection of soybean as affected by stage of plant development. Phytopathology 62(7):771

    Google Scholar 

  • Limonard T (1966) A modified blotter test for seed health. Neth J Plant Pathol 72(3–6):319–321

    Article  Google Scholar 

  • Mangan A (1971) A new method of the detection of Pleospora bjoerlingii infection of sugar-beet seed. Trans Br Mycol Soc 57:169–172

    Article  Google Scholar 

  • Marcinkowska JZ (2002) Methods of finding and identification of pathogens in seeds. Plant Breed Seed Sci 46(1):31–48

    Google Scholar 

  • Maury Y, Duby C, Bossennec JM et al (1985) Group analysis using ELISA: determination of the level of transmission of Soybean mosaic virus in soybean seed: seed group size and seed decotication. Agronomie 7:225–230

    Article  Google Scholar 

  • Maury Y, Duby C, Khetarpal RK (1998) Seed certification for viruses. In: Hadidi A, Khetarpal RK, Koganezawa H (eds) Plant virus disease control. APS Press, Saint Paul, pp 237–248

    Google Scholar 

  • Muskett AE, Malone JP (1941) The ulster method for the examination of flax seed for the presence of seed-borne parasites. Ann Appl Biol 28:8–13

    Article  Google Scholar 

  • Nath R, Neergaard P, Mathur SB (1970) Identification of Fusarium species on seeds as they occur in the blotter test. Proc Int Seed Test Assoc 35:121–144

    Google Scholar 

  • Neergaard P (1977) Seed pathology. Volume I and II. Macmillan, London, p 1187

    Google Scholar 

  • Ou SH (1963) FAO (Expanded Programme of Technical Assistance) Rep. 1673, p 28

    Google Scholar 

  • Popp W (1958) An improved method of detecting loose-smut mycelium in whole embryos of wheat and barley. Phytopathology 48(12):641–643

    Google Scholar 

  • Rice WN (1939) The hemocytometer method for detecting fungus spore load carried by wheat. Proc Assoc Off Seed Anal N Am 31:124–127

    Google Scholar 

  • Russel RC (1950) The whole embryo method for testing barley for loose smut as a routine test. Sci Agric 30:361–366

    Google Scholar 

  • Simmonds PM (1946) Detection of the loose smut fungus in embryos of barley and wheat. Sci Agric 26:51–58

    Google Scholar 

  • Skvortzov SS (1937) A simple method for detecting hyphae of loose smut in wheat grains. Plant Protect Leningr 15:90–91, 36: 51–58

    Google Scholar 

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Kumar, A., Gaur, A., Gurjar, M.S., Kumari, P., Kiran, R. (2020). Seed Health Testing and Seed Certification. In: Kumar, R., Gupta, A. (eds) Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management. Springer, Singapore. https://doi.org/10.1007/978-981-32-9046-4_27

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