Abstract
Antioxidant enzymes are involved in the therapy of the cardiovascular diseases associated with increased production of reactive oxygen species (ROS). The enzyme efficiency is dependent on the bioactive molecule’s ability to achieve therapeutically adequate levels at the target site. In this paper, superoxide dismutase (SOD) and catalase, enzymes involved in decomposition of reactive oxygen species, have been immobilized onto superparamagnetic iron oxide nanoparticles(SPIONs) and characterized. FT-IR data, particles mean diameter and zeta potential confirmed the enzymes immobilization onto SPIONs. The particles dimensions vary from 400nm for catalase immobilization to 1ìm for SOD immobilization. The particles morphology was studied by scanning electron microscopy (SEM) and suggests the particles tendency for clustering during the drying process. The biofunctinalized SPIONs are degraded by esterases. The citocompatibility tests (MTT) indicated normal values for the cells viability in presence of SPIONs with immobilized enzymes. Enzymatic activity of magnetic particles has been determined indirect by NTBmethionine-riboflavin (in the case of superoxide dismutase) and direct, with H2O2 (for catalase). EnzySPIONs preserve an enzymatic activity which is dependent of the magnetic particles concentration, the enzyme nature and biological molecule conformation onto magnetic particle.
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© 2016 Springer Science+Business Media Singapore
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Lacramioara, L., Diaconu, A., Butnaru, M., Verestiuc, L. (2016). Biocompatible SPIONs with Superoxid Dismutase/Catalase Immobilized for Cardiovascular Applications. In: Sontea, V., Tiginyanu, I. (eds) 3rd International Conference on Nanotechnologies and Biomedical Engineering. IFMBE Proceedings, vol 55. Springer, Singapore. https://doi.org/10.1007/978-981-287-736-9_78
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DOI: https://doi.org/10.1007/978-981-287-736-9_78
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Publisher Name: Springer, Singapore
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Online ISBN: 978-981-287-736-9
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