Evaluation of Nutraceuticals in Fruit Extracts of Psidium Guajava L

  • A. A. Haleem Khan
  • Naseem
  • B. Vidya Vardhini
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)


Fruits and vegetables are the food sources that serve as healthy food to provide additional physiological benefit, beyond the basic nutrition due to their richness in bioactive phytochemicals such as polyphenols and carotenoids that serve both nutrients as well as protection against maladies. Psidium guajava L. plant belongs to the family Myrtaceae, commonly known as guava is cultivated as flora in tropical and sub tropical region. The fruits of this plant are consumed daily and known for its medicinal value from folk medicine. The aim of present study was to analyze the phytochemical constituents in extracts of guava fruit. The extracts under study were made from fruit peel, pulp, and seeds of guava fruit by employing solvents like ethanol, ethyl acetate, methanol, sterile distilled water. The parameters studied are organoleptic characters, extractive values, phytochemical constituents [alkaloids, flavonoids, saponins, tannins, glycosides, and amino acids] were analyzed in the variety of fruit extracts. The total phenolic content of fruit extracts was evaluated spectrophotmetrically according to Folin-Ciocalteu phenol method and expressed in gallic acid equivalents [GAE]. Results showed that organoleptic characters were the color for fruit peel was green, pulp was white and seeds were creamy and the taste was bitter and palatable. The odor was leafy and aroma and the texture were rough. The extractive values, phytochemical screening and phenol content for extracts from peel, pulp and seeds was in better alcoholic solvents like ethanol and methanol. This study reveals the P. guajava fruit extracts are moderate potential sources of natural antioxidants.


Nutraceuticals Psidium guajava Fruit extracts 


  1. 1.
    Bontempo P, Doto A, Miceli M, Mita L, Benedetti R, Nebbioso A, Veglione M, Rigano D, Cioffi M, Sica V, Molinari AM, Altucci L (2012) Psidium guajava L. anti-neoplastic effects: induction of apoptosis and cell differentitation. Cell Prolif 45:22–31CrossRefGoogle Scholar
  2. 2.
    Chen H-Y, Yen G-C (2007) Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves. Food Chem 101(2):686–694CrossRefGoogle Scholar
  3. 3.
    Chen HC, Sheu MJ, Lin LY, Wu CM (2007) Chemical composition of the leaf essential oil of Psidium guajava L. from Taiwan. J Essent Oil Res 19:345–347CrossRefGoogle Scholar
  4. 4.
    Cole RA, Setzer WN (2007) Chemical composition of the leaf essential oil of Psidium guajava from Monteverde, Costa Rica. J Essent Oil-Bear Plants 10:365–373CrossRefGoogle Scholar
  5. 5.
    de Lima RK, Cradoso MdG, Andrade MA, Nascimento EA, de Morais SAL, Nelson DL (2010) Composition of the essential oil from from the leaves of tree domestic varieties and one wild variety of the guava plant (Psidium guajava L., Myrtaceae). Revista Brasileira de Farmacognosia Brazilian J Pharmacognosy 20(1): 41–44Google Scholar
  6. 6.
    El-Ahmady SH, Ashour ML, Wink M (2013) Chemical composition and anti-inflammatory activity of the eseential oils of Psidium guajava fruits and leaves. J Essent Oil ResGoogle Scholar
  7. 7.
    Flores G, Dastmalchi K, Wu S-B, Whalen K, Aj Dabo, Reynertson RF, Foronjy JM, D’Armiento JM, Kennelly EJ (2013) Phenolic-rich extract from the Costa Rican guava (Psidium friedrichsthalianum) pulp with antioxidant and anti-inflammatory Potential for COPD therapy. Food Chem 141:889–895CrossRefGoogle Scholar
  8. 8.
    Ghosh R, Tiwary BK, Kumar A, Chakraborty R (2014) Guava leaf extracts inhibits quorum-sensing and Chromobacterium violaceum induced lysis of human hepatoma cells: whole transcriptome analysis reveals differential gene expression. PLoS ONE 9(9):e107703CrossRefGoogle Scholar
  9. 9.
    Khadhri A, El Mokni R, Almeida C, Nogueira JMF, Araujo MEM (2014) Chemical composition of essential oil of Psidium guajava L. growing in Tunisia. Ind Crops Prod 52:29–31CrossRefGoogle Scholar
  10. 10.
    Lee WC, Mahmud R, Noordin R, Piaru SP, Perumal S, Ismail S (2013) Free radicals scavenging activity, cytotoxicity and anti-parasitic activity of essential oil of Psidium guajava L. leaves against Toxoplasma gondii TEOP 16(1): 32–38Google Scholar
  11. 11.
    Lee WC, Mahmud R, Pillai S, Perumal S, Ismail S (2012) Antioxidant activities of essential oil of Psidium guajava L. leaves APCBEE Procedia 2: 86–91Google Scholar
  12. 12.
    Lee BH, Annis PC, Tumaalii F, Choi WS (2004) Fumigant toxicity of essential oils from the Myrtaceae family and 1,8-cineole against 3 major stored-grain insects. J Stored Prod Res 40:553–564CrossRefGoogle Scholar
  13. 13.
    Lozoya X, Reyes-Morales H, Chavez-Soto MA, Martínez-Garcia MC, Soto-Gonzalez Y, Doubova SV (2002) Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease. J Ethnopharmacol 83:19–24CrossRefGoogle Scholar
  14. 14.
    Manosroi J, Dhumtanom P, Manosroi A (2006) Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Lett 235:114–120CrossRefGoogle Scholar
  15. 15.
    Musa KH, Abdullah A, Jusoh K, Subramaniam V (2011) Antioxidant activity of pink-flesh guava (Psidium guajava L.): Effect of extraction techniques and solvents. Food Anal Meth 4:100–107CrossRefGoogle Scholar
  16. 16.
    Ogunwande IA, Olawore NO, Adeleke KA, Ekundayo O, Koenig WA (2003) Chemical composition of the leaf volatile oil of Psidium guajava L. growing in Nigeria. Flavor Frag J 18:136–138CrossRefGoogle Scholar
  17. 17.
    Ojewole JAO, Awe EO, Chiwororo WDH (2008) Antidiarrhoeal activity of Psidium guajava Linn. (Myrtaceae) leaf aqueous extract in rodents. J Smooth Muscle Res 44(6):195–207CrossRefGoogle Scholar
  18. 18.
    Oh WK, Lee CH, Lee MS, Bae EY, Sohn CB, Oh H (2005) Antidiabetic effects of extracts from Psidium guajava. J Ethnopharmacol 96:411–415CrossRefGoogle Scholar
  19. 19.
    Pino JÁ, Aguero J, Marbot R, Fuentes V (2001) Leaf oil of Psidium guajava L. from Cuba. J Essent Oil Res 31:61–62CrossRefGoogle Scholar
  20. 20.
    Prabu GR, Gnanamani A, Sadulla S (2006) Guaijaverin-aplant flavonoids as potential antiplaques agent against Streptococcus mutans. J Appl Microbiol 101:487–495CrossRefGoogle Scholar
  21. 21.
    Ryu NH, Park KR, Kim SM, Yun HM, Nam D, Lee SG, Hj Jang, Ahn KS, Kim SH, Shim BS, Choi SH, Mosaddik A, Cho SK, Ahn KS (2012) A hexane fraction of guava leaves (Psidium guajava L.) induces anticancer activity by suppressing AKT/Mammalian target of Rapamycin/Ribosomal p70 S6 kinase in human prostrate cancer cells. J Med Food 15(3):231–241CrossRefGoogle Scholar
  22. 22.
    Seo J, Lee S, Elam ML, Johnson SA, Kang J, Arjmandi BH (2014) Study to find the best extraction solvent for use with guava leaves (Psidium guajava L.) for high antioxidant efficacy. Food Sci Nutr 2(2):174–180CrossRefGoogle Scholar
  23. 23.
    Sacchetti G, Maietti S, Muzzoli MV, Scaglianti M, Manfredini S, Radice M, Bruni R (2005) Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 91:621–632CrossRefGoogle Scholar
  24. 24.
    Shen SC, Chen FC, Wu NJ (2008) Effect of guava (Psidium guajava Linn.) leaf soluble solids on glucose methabolism in type 2 diabetic rats. Phytother Res 22:1458–1464CrossRefGoogle Scholar
  25. 25.
    Silva JD, Luz AIR, Silva MHL, Andrade EHA, Zoghbi MGB, Maia JGS (2003) Essential oils of the leaves and stems of four Psidium spp. Flavor Frag J 18:240–243CrossRefGoogle Scholar
  26. 26.
    Siwarungson N, Ali N, Damsud T (2013) Comparative analysis of antioxidant and antimelanogenesis properties of three local guava (Psidium guajava L.) varieties of Thailand, via different extraction solvents. Food Meas 7:207–214CrossRefGoogle Scholar

Copyright information

© The Author(s) 2016

Authors and Affiliations

  1. 1.Department of BotanyUniversity College, Telangana UniversityNizamabadIndia
  2. 2.Department of Pharmaceutical ChemistryUniversity College, Telangana UniversityNizamabadIndia

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