Novel Topical Gel Formulation of Hamiltonia Suaveolens for Wound Healing Activity
Wound healing is a complex multifactorial process that results in the contraction and closure of the wound and restoration of functional barrier. Hamiltonia Suaveolens roots are widely used in Indian medicine to cure wounds, burns. However, complete evaluation of these activities is absent. Thus, the present study aimed to assess the wound healing activity of Hamiltonia Suaveolens roots in topical ointments formulation. The gel formulation containing extracts of the above mentioned herb were formulated, the gel formulation containing extracts evaluated and their wound healing activity was studied on experimentally induced excision open wounds in albino rats. The effect of the formulation on wound healing was assessed by the rate of wound closure, periods of epithelialization and histopathology. The aqueous extract of Hamiltonia suaveolens roots was incorporated into Carbopol 934-water mixture. The pH, viscosity, spreadability, stability studies, acute oral toxicity, and skin irritation test were determined. The measurement of the wound areas were taken up-to 18th days and the percentages of the wound closures were calculated. Blank ointment base and Gentamycin ointment (1 % w/w) served as the control and standard treatments. The prepared ointments passed all the physical parameters. There were no changes in pH, viscosity, spreadability and consistency when the ointments were kept at different temperatures for 90 days. These formulations did not produce any skin irritation. Topical applications of the prepared ointment on the excision wound in rats caused a significantly higher rate of wound healing and reduced the epithelialization period in a dose-related manner. The tissue histology of gel treated group showed complete epithelialization with increased collagenation, compared to the (1 %) Gentamycin ointment. The formulated gel was evaluated and effective in wound care and should be explored in harnessing the potentials of the plant in the treatment of topical diseases.
KeywordsWound healing Herbal formulation Hamiltonia suaveolens roots Excision wound
- 1.Pascal M, Anthony CD (2008) Mechanisms involved in wound healing. Focusing on injuries, pp 609–615Google Scholar
- 2.Shafiuddin Md, Abdullah K, Sadath A (2009) Wound healing activity of traditional herbal formulation. Int J Chem Sci 7(2):639–643Google Scholar
- 4.Cines DB, Pollack ES, Buck CA (1998) Endothelial cells in physiology and in the pathophysiology of vascular disorder. Blood 91:3527–3561Google Scholar
- 5.White JD, Gerrard JM (1976) Ultrastructural features of abnormal platelets: a review. Am J Pathol 83:589–632Google Scholar
- 9.Stiernbery J, Redin WR, Warner WS (1993) The role of thrombin and thrombin receptor activating peptide (TRAP-508) in initiation of tissue repair. Tromb Haemost 70:158–162Google Scholar