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Study of Genetic Polymorphism at 8 STR Loci in the Jat Sikh Population of Madhya Pradesh

  • Pankaj Shrivastava
  • Devika Dogra
  • Ruchira Chaudhary
Chapter
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Abstract

Allele frequencies for 8 autosomal Short Tandem Repeat loci namely D18S51, D21S11, FGA, CSF1PO, D16S539, D7S820, D13S317, D2S1338 and Amelogenin, were studied in 50 unrelated individuals from the Jat Sikh population of M.P. PCR amplification was performed with the AmpFlSTR® MinifilerTM PCR Amplification system and the amplified products were separated by ABI 3100 DNA genetic analyzer. No significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed for 8 STR loci analyzed (P-value > 0.05). None of the P-values could be considered significant. No linkage disequilibrium was found between the loci as all loci are located on the different chromosome, and thus they are statistically independent. The F IS value for Jat Sikh population has been calculated as 0.005. This low F IS value indicates that the population is in random mating with high level of heterozygosity. The power of discrimination of the microsatellite markers used was found to be high for the studied populations. The data thereof is of significance for forensic result interpretation and is an addition to the existing autosomal STR database on the Indian population.

Keywords

AmpFLSTR® MinifilerTM PCR amplification kit Autosomal STR Jat sikh 

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Copyright information

© The Author(s) 2016

Authors and Affiliations

  • Pankaj Shrivastava
    • 1
  • Devika Dogra
    • 2
  • Ruchira Chaudhary
    • 3
  1. 1.DNA Fingerprinting UnitState Forensic Science LaboratorySagarIndia
  2. 2.Department of BiotechnologyBarkatullah UniversityBhopalIndia
  3. 3.Biotechnology Division, Department of ZoologyGovernment Motilal Vigyan MahavidyalayaBhopalIndia

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