Biopriming of Micropropagated Plantlets Using Various Bacterial Strains in Different Combinations-Induces the Systemic Resistance

  • Sunitha Panigrahi
  • K. Aruna Lakshmi
  • Nida Mir
  • Maria Taiyebi
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)


Plants prone to high mortality rate and rapid disease exposure lead to a huge agricultural loss. This loss is minimized by various techniques adopted by the modern technological world. Micropropagation is one such technique that has the potential to provide huge growth rates of a particular plant genotype. It not only helps for the rapid increment in plant growth but also a longer life span for the modified plants, hence, contributing to the world wide agricultural advancement. When these plants are transferred exposed to ex-vitro conditions they suffer a huge loss due to environmental changes. The abiotic and biotic stress takes a toll on these tissue cultured plants. Biohardening is a rescue method for such plants. Biohardening also known as biopriming is a technique that involves inoculation of various bacterial strains that help the plants against ex-vitro conditions. Plants when grown in vitro are provided with ideal conditions necessary for their growth, these plants are delicate with undergrown dermal covering hence, and they are highly susceptible to diseases and growth hindrance when removed from the in vitro environment. Thus, hardening and acclimatization of in vitro grown plantlets is a necessary phase in micro propagation. The plantlets inoculated with the beneficial bacterial strains not only become resistant to the adverse environmental conditions but also attain a higher growth rate due to the fixation of minerals and nutrients by the bacteria. The manipulated conditions contradict the previous fragile nature, strengthening the plant and increasing the growth output. This paper will enlighten you about the effects of various growth promoting bacteria that have been used in acclimatizing the tissue cultured plants. These bacteria’s were previously experimented using single, dual, triple and quadruple combinations which showed humongous success in plant growth manipulation. In the present experiment, the same bacteria’s were combined in sets of 5 bacteria’s and consortium. These bacteria’s involve themselves in a mutually beneficial relationship with the plants. They help the plants acquire the required minerals and nutrients which otherwise wouldn’t have been consumed. The growth rate regarding the morphological and physiological characteristics were recorded and documented. Further, the plant manipulation and growth factors were observed.


Micro propagation Acclimatization Biohardening Rhizobium VAM 


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Copyright information

© The Author(s) 2016

Authors and Affiliations

  • Sunitha Panigrahi
    • 1
  • K. Aruna Lakshmi
    • 2
  • Nida Mir
    • 3
  • Maria Taiyebi
    • 3
  1. 1.Department of Life ScienceSt. Mary’s CollegeHyderabadIndia
  2. 2.Department of BiotechnologyGITAM UniversityHyderabadIndia
  3. 3.St. Mary’s CollegeHyderabadIndia

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