Acclimatization of Invitro Propagated Banana Grand Naine by Biotinization—Survival Rate by Phenol Concentrations

  • Sunitha Panigrahi
  • K. Aruna Lakshmi
  • V. Madhuri
  • A. N. Soumya
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)


Despite advances made in micropropagation technology acclimatization/hardening continues to be the bottleneck for commercializing tissue culture plants. When invitro grown plants are transferred and exposed to ex-vitro conditions they suffer a huge loss due to environmental changes. The abiotic and biotic stress takes a toll on these tissue cultured plants. “Biotization” is a technique analogous to vaccination where physiological changes are induced in the plants by plant growth promoting bacteria. Thereby, enhancing the tolerance to the abiotic and biotic stress. The present study evaluates the efficacy of bacterial and fungal consortium on improving ex vitro primary hardening in banana variety “Grand Naine”. The beneficial bacteria were Azotobacter, Azospirullum, Rhizobium, Pseudomonas, Phosphate Solubilizing Bacteria and VAM. 64 treatments were given (T0–T64) as single, dual, triple quadruple and consortium of all six micro-organisms. Commercial formulations of bacteria were mixed with the potting medium used for hardening the banana plants at a concentration of 107 CFU per gram of soil. The parameters studied were survival rate, root length, shoot length, fresh weight, dry weight, total proteins, micro and macronutrients- N, P, K, Phenols. Our results suggest that all treatments enhanced the survival and growth over the control. Best growth was however obtained in t-64 which contained all the 5 bacteria and VAM. Consortium enhanced root growth by 30 %, shoot growth by 50 %, fresh weight and dry weight by 20 %, protein by 20 %, Micro and Macronutrients by 30 %, Phenols by 20 %, Survival rate by 80 % over the control. Enhanced mineral uptake probably lead to extensive root and shoot growth. Increased phenol content presumably induced resistance to wilt infection. “Biotization” is a potential technique which can induce systemic resistance to abiotic and biotic stress in plants leading to better survival during hardening. Routine implementation of this technique in commercial micropropagated units can reduce the mortality rate of invitro propagated plants during hardening.


Systemic resistance Micropropagated plantlets Bacterial strains 


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Copyright information

© The Author(s) 2016

Authors and Affiliations

  • Sunitha Panigrahi
    • 1
  • K. Aruna Lakshmi
    • 2
  • V. Madhuri
    • 1
  • A. N. Soumya
    • 1
  1. 1.Department of BiotechnologySt. Mary’s CollegeHyderabadIndia
  2. 2.Department of BiotechnologyGitam UniversityVisakhapatnamIndia

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