Crime Scene Forensic Evidence as Trace DNA Profiling Solves the Case

  • K. P. Mahajan
  • V. B. Parulekar
  • S. H. Lade
  • M. K. Malave
Chapter
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Abstract

Any case to be analysed depends on remnants of deceased or samples found at crime scene. Hair, beer bottles, cigarette butts or bidi butts are most common evidences that can be left behind by accused. Finding culprits using these evidences becomes most challenging exercise. Extraction of DNA is a very important task in forensic analysis. Chances of obtaining contaminated DNA from saliva detected on bidi butts are higher. Bidi butts contain PCR inhibitors like tars and phenolics from the smoke. So it is a challenge to extract good quality DNA in one stroke. In present case two bidi butts found at scene of crime was the only evidence to detect the accused. Amplifiable DNA was extracted from saliva detected on bidi butt with the use of optimized and validated DNA extraction protocol.

Keywords

DNA extraction PCR Saliva 

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Copyright information

© The Author(s) 2016

Authors and Affiliations

  • K. P. Mahajan
    • 1
  • V. B. Parulekar
    • 1
  • S. H. Lade
    • 1
  • M. K. Malave
    • 1
  1. 1.Directorate of Forensic Science LaboratoriesMumbaiIndia

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