Abstract
Microbial fuel cells (MFC) is a bio-electrochemical device that converts organic substrate into electricity by electrogenic bacteria. In the MFC, anode compartment plays an important role in achieving high power density and thereby improving the cell performance. In this study, Geobacter sulfurreducens was used as a biocatalyst to catalyze the generation of electricity by using acetate as an oxidizing agent and sodium fumarate as a reducing agent. The polarization curve was determined after MFC system was reached at open circuit voltage (OCV) around 0.80 V and gives maximum power production of about 0.22 mW. The cyclic voltammetry (CV) method was performed in order to evaluate the oxidation and reduction processes in a batch of MFC before and after attachment of Geobacter sulfurreducens cells and it was proved by scanning electron microscope (SEM) images. Overall, this study provides a convenient way for evaluating the performance of MFC.
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References
Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192
Nicholson RS (1965) Theory and application of cyclic voltammetry for measurement of electrode reaction kinetics. Anal Chem 37:1351–1355
Zhou M, Chi M, Luo J, He H, Jin T (2011) An overview of electrode materials in microbial fuel cells. J Power Sour 196:4427–4435
Liu X-W, Sun X-F, Huang Y-X, Sheng G-P, Zhou K, Zeng RJ, Dong F, Wang S-G, Xu A-W, Tong Z-H (2010) Nano-structured manganese oxide as a cathodic catalyst for enhanced oxygen reduction in a microbial fuel cell fed with a synthetic wastewater. Water Res 44:5298–5305
Kim JR, Jung SH, Regan JM, Logan BE (2007) Electricity generation and microbial community analysis of alcohol powered microbial fuel cells. Bioresour Technol 98:2568–2577
Rabaey K, Ossieur W, Verhaege M, Verstraete W (2005) Continuous microbial fuel cells convert carbohydrates to electricity. Water Sci Technol 52:515–523
Marsili E, Rollefson JB, Baron DB, Hozalski RM, Bond DR (2008) Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms. Appl Environ Microbiol 74:7329–7337
Pham CA, Jung SJ, Phung NT, Lee J, Chang IS, Kim BH, Yi H, Chun J (2003) A novel electrochemically active and Fe (III)-reducing bacterium phylogenetically related to Aeromonas hydrophila, isolated from a microbial fuel cell. FEMS Microbiol Lett 223:129–134
Fricke K, Harnisch F, Schröder U (2008) On the use of cyclic voltammetry for the study of anodic electron transfer in microbial fuel cells. Energy Environ Sci 1:144–147
Marsili E, Sun J, Bond DR (2010) Voltammetry and growth physiology of Geobacter sulfurreducens biofilms as a function of growth stage and imposed electrode potential. Electroanalysis 22:865–874
Harnisch F, Freguia S (2012) A basic tutorial on cyclic voltammetry for the investigation of electroactive microbial biofilms. Chem Asian J 7:466–475
Oh S, Kim J, Joo J, Logan B (2009) Effects of applied voltages and dissolved oxygen on sustained power generation by microbial fuel cells. Water Sci Technol 60:1311
Logan BE (2008) Microbial fuel cells. Wiley, New York
Nimje VR, Chen C-C, Chen H-R, Chen C-Y, Tseng M-J, Cheng K-C, Shih R-C, Chang Y-F (2012) A single-chamber microbial fuel cell without an air cathode. Int J Mol Sci 13:3933–3948
Bond DR, Lovley DR (2003) Electricity production by Geobacter sulfurreducens attached to electrodes. Appl Environ Microbiol 69:1548–1555
Cho HK, Trinh NT, An JY, Kim BW (2008) Optimizing the power density generated from a microbial fuel cell using Geobacter sulfurreducens with acetate. J Biotechnol 136(Supplement):S485
Nevin KP, Richter H, Covalla S, Johnson J, Woodard T, Orloff A, Jia H, Zhang M, Lovley D (2008) Power output and columbic efficiencies from biofilms of Geobacter sulfurreducens comparable to mixed community microbial fuel cells. Environ Microbiol 10:2505–2514
Kim M-S, Lee Y-J (2010) Optimization of culture conditions and electricity generation using Geobacter sulfurreducens in a dual-chambered microbial fuel-cell. Int J Hydrogen Energy 35:13028–13034
Trinh NT, Park JH, Kim B-W (2009) Increased generation of electricity in a microbial fuel cell using Geobacter sulfurreducens. Korean J Chem Eng 26:748–753
Logan BE (2009) Exoelectrogenic bacteria that power microbial fuel cells. Nat Rev Microbiol 7:375–381
Logan BE, Regan JM (2006) Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol 14:512–518
Acknowledgment
This research was supported by funding through the Fundamental Research Grant Scheme (FRGS) (Acc Number: 6071232) by Ministry of Higher Education, Malaysia and Postgraduate Research Grant Scheme (PRGS) (Acc Number: 8046005) by Universiti Sains Malaysia (USM).
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Shoparwe, N.F., Uzir, M.H., Abdul Sata, S. (2015). Performance Studies of Anode Microbial Fuel Cells Using Geobacter Sulfurreducens as a Biocatalyst. In: Hashim, M. (eds) ICGSCE 2014. Springer, Singapore. https://doi.org/10.1007/978-981-287-505-1_41
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DOI: https://doi.org/10.1007/978-981-287-505-1_41
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