Effect of Plant Growth Regulators on Morphological, Physiological and Biochemical Parameters of Soybean (Glycine max L. Merrill)

  • R. RameshEmail author
  • E. Ramprasad
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)


Plant growth and developmental processes are very much regulated by certain chemical substances called Growth regulators. Growth regulators are known to improve the physiological efficiency including photosynthetic ability and can enhance the effective partitioning of accumulates from source and sink in the field crops. The present study was conceptualized and executed with the prime objective of study the effect of chlormequat chloride, NAA, Mepiquat chloride and Brassinosteroids on morphological, physiological and biochemical parameters of soybean. The field trial was conducted following randomized block design with nine treatments replicated thrice. The basic material for the present investigation consisted of soybean cv. Js-335 and two growth promoting (NAA and Brassinosteroid) and growth retarding substances (chlormequat chloride and mepiquat chloride). These growth regulators were sprayed at flower initiation stage. The Morphophysiological parameters, namely, Plant height, number of branches, number of trifoliates per plant, dry matter accumulation in leaf, stem and reproductive parts, LAI, CGR and RGR was observed to increase significantly with the application of NAA (20 ppm) and brassinosteroid (25 ppm). However, it decreased with the application of chlormequat chloride and mepiquat chloride. The Biochemical parameters, namely, chlorophyll content was observed to increase significantly with application of NAA (20 ppm), brassinosteroid (25 ppm), mepiquat chloride 5 %, AS (5 %) and chlormequat chloride 50 % SL at different concentrations compared to control and water spray but whereas fluorescence emission and photosynthetic rate were noticed to be non-significant. A significant increase in the seed protein content was also noticed with the application of NAA (20 ppm), brassinosteroid (25 ppm), mepiquat chloride 5 %, AS (5 %) and chlormequat chloride at different concentrations, compared to control and water spray. In conclusion, the study revealed the superiority of NAA (20 ppm) treatment for majority of the morphological, physiological and biochemical parameters at different growth stages, compared to other growth regulator and control treatments studied in the present investigation for Rabi soybean.


Growth regulators Randomized block design Soybean cv. Js-335 Morphophysiological parameters Biochemical parameters 


  1. 1.
    Association of official analytical chemists: AOAC (1980) Official methods of analysis, 12th edn. William Star Wet Glad (edn), WashingtonGoogle Scholar
  2. 2.
    Panse V, Sukhatme PV (1989) Statistical method for agricultural workers. ICAR, New Delhi, p 108Google Scholar
  3. 3.
    Lakshmamma P, Rao IVS (1996) Influence of shading and naphthalene acetic acid (NAA) on yield and yield components in black gram (Vigna mungo L.). Ann Agric Res 17:320–321Google Scholar
  4. 4.
    Shukla KC, Singh OP, Samaiya RK (1997) Effect of foliar spray of plant growth regulator and nutrient complex on productivity of soybean var. JS 79–81. Crop Res 13:213–215Google Scholar
  5. 5.
    Govindan K, Thirumurugan V, Aruchelvan S (2000) Response of soybean to growth regulators. Res Crops 1(3):323–325Google Scholar
  6. 6.
    Deotale RD, Katekhaye DS, Sorte NV, Raut JS, Golliwar VJ (1995) Effect of TIBA and B-Nine on morpho-physical characters of soybean. J Soils Crops 5:172–176Google Scholar
  7. 7.
    Rajesh K (2010) Influence of growth promoting and retarding compounds on growth, dry matter production and yield in green gram during rabi Google Scholar
  8. 8.
    Das A, Prasad R (2004) Effect of plant growth regulators on green gram (Phaseolus radiatus). Indian J Agri Sci 74(5):271–272Google Scholar
  9. 9.
    Girisha 2010 A comparative study on the growth promoting and retarding compounds on dry matter production and yield in black gram during rabi. M.Sc.(Ag) thesis submittedGoogle Scholar
  10. 10.
    Merlo D, Soldati A, Keller ER (1987) Influence of growth regulators on abscission off lower and young pods of soybeans. Eurosaya 5:31–38Google Scholar
  11. 11.
    Chandrasekar CN, Bangarusamy U (2003) Maximizing the yield of mung bean by foliar application of growth regulating chemicals and nutrients. Madras Agric J 90(1–3):142–145Google Scholar
  12. 12.
    Kumar P, Hiremath SM, Chetti MB (2006) Influence of growth regulators on dry matter production and distribution and shelling percentage in determinate and semi determinate soybean genotypes. Legume Res 29(3):191–195Google Scholar
  13. 13.
    Kothule VG, Bhalerao RK, Rathod TH (2003) Effect of growth regulators on yield attributes, yield and correlation coefficients in soybean. Ann Plant Physiol 17(2):140–142Google Scholar
  14. 14.
    Kumbhare MD, Khawale VS, Rajput GR, Datey CP, Dapugeanti KG (2007) Effect of nitrogen levels and chlormequat on mustard (Brassica juncea L.). J Soils Crops 17(2):394–397Google Scholar
  15. 15.
    Patil SB (1994) Effect of population levels and growth retardants on growth, yield and yield attributes and quality of soybean. M.Sc. (Agric) thesis, University of Agricultural Sciences, DharwadGoogle Scholar
  16. 16.
    Jeyakumar P, Thangaraj M (1998) Physiological and biochemical effects of mepiquat chloride in groundnut (Archis hypogaea). Madras Agric J 85:23–26Google Scholar
  17. 17.
    Dong DF, Li YR, Jiang LG (2008) Effects of brassinosteroids on photosynthetic characteristics in soybean under aluminum stress. Acta Agron Sin 34(9):1673–1678Google Scholar
  18. 18.
    Shalaby MAF (2000) Influence of cycocel (2-chloroethyl ammonium chloride) on the vegetative growth, photo synthetic pigments, flowering, abscission and yield of faba bean (Vicia faba, L.). Ann Agric Sci Moshtohor, 38(3):1485–1502Google Scholar

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© The Author(s) 2015

Authors and Affiliations

  1. 1.Department of Crop PhysiologyAcharya N. G. Ranga Agricultural UniversityHyderabadIndia
  2. 2.Department of Plant Molecular Biology and BiotechnologyAcharya N. G. Ranga Agricultural UniversityHyderabadIndia

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