Anti-cancer Activity of Selected Seaweeds Against HeLa, K-562 and MDA-MB Cell Lines

Chapter
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Abstract

Anticancer activity of marine macro algae (seaweeds) Sargassum wightii, Ulva fasciata and Gracillaria corticata against HeLa, K-562 and MDA-MB cell lines was studied. During the present investigation crude extracts of seaweeds were prepared using soxhlet apparatus. Hexane, Butanol and Methanol were used as solvents. Crude extracts were dissolved in DMSO. In vitro anticancer activity of seaweeds at various concentrations (12.5–200 μg/ml) was studied against the chosen cell lines using MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole). Butanolic extract of Gracillaria corticata (200 µg/ml) has shown greater anticancer activity than hexane and methanolic extracts of G. corticata and butanol, hexane and methanolic extracts of Sargassum wightii and Ulva fasciata.

Keywords

Seaweeds HeLa K-562 and MDA-MB cell 

Notes

Acknowledgment

The first author thanks the Management and the Principal of G.V.P. College for Degree and P.G. Courses, for the encouragement and support, Prof. A. Shameem and Dr. P. Janaki Ram, Department of Marine Living Resources, Andhra University for their guidance and Prof. P. Sasi Bhushana Rao for guidance and critically going through the manuscript and suggestions.

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Copyright information

© The Author(s) 2015

Authors and Affiliations

  1. 1.Department of BiochemistryGayatri Vidya Parishad Degree College (Autonomous)VisakhapatnamIndia
  2. 2.Department of Marine Living ResourcesAndhra UniversityVisakhapatnamIndia

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