Abstract
The capability to capture the evolution of the structure and properties of nanomaterials in response to external stimuli makes in situ transmission electron microscopy ideally suited to tackle the present challenges of the nanoworld. In situ transmission electron microscopy has become one of the most powerful approaches for revealing physical and chemical process dynamics at atomic resolution. This chapter gives an overview of in situ transmission electron microscopy. The background, the early history, and the current status of in situ transmission electron microscopy are briefly reviewed, and the challenges, as well as future opportunities, are discussed.
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References
Porter JR (1976) Antony van Leeuwenhoek: tercentenary of his discovery of bacteria. Bacteriol Rev 40(2):260–269. https://doi.org/10.1128/MMBR.40.2.260-269.1976
De Broglie L (1923) Waves and quanta. Nature 112(2815):540–540. https://doi.org/10.1038/112540a0
Busch H (1927) Über die Wirkungsweise der Konzentrierungsspule bei der Braunschen Röhre. Archiv für Elektrotechnik 18(6):583–594. https://doi.org/10.1007/bf01656203
Knoll M, Ruska E (1932) Das elektronenmikroskop. Zeitschrift für physik 78(5–6):318–339. https://doi.org/10.1007/BF01330526
Marton L (1935) La microscopie electronique des objets biologiques. Académie Royale de Belgique: Bulletin de la Classe des Sciences 21:553–564
Ruska E (1942) Beitrag zur übermikroskopischen Abbildung bei höheren Drucken. Kolloid-Zeitschrift 100(2):212–219. https://doi.org/10.1007/bf01519549
von Ardenne M (1942) Reaction chamber over microscopism with the universal electron microscope. Z Phys Chem B-Chem Elem Aufbau Mater 52(1–2):61–71
Hashimoto H, Naiki T, Eto T, Fujiwara K (1968) High temperature gas reaction specimen chamber for an electron microscope. Jpn J Appl Phys 7(8):946–952. https://doi.org/10.1143/jjap.7.946
Moretz RC, Hausner G, Johnson HM, Parsons DF (1969) Design of a wet specimen chamber for investigation of hydrated specimens in electron microscope. Biophys J 9:A194
Swann P, Tighe N (1971) High voltage microscopy of gas oxide reactions. Jernkontorets Annaler 155(8):497–501
Abrams IM, McBain JW (1944) A closed cell for electron microscopy. J Appl Phys 15(8):607–609. https://doi.org/10.1063/1.1707475
Williamson MJ, Tromp RM, Vereecken PM, Hull R, Ross FM (2003) Dynamic microscopy of nanoscale cluster growth at the solid-liquid interface. Nat Mater 2(8):532–536. https://doi.org/10.1038/nmat944
Burton E, Sennett R, Ellis S (1947) Specimen changes due to electron bombardment in the electron microscope. Nature 160(4069):565–567. https://doi.org/10.1038/160565b0
Hamm FA, Norman EV (1948) Transformations in organic pigments. J Appl Phys 19(12):1097–1109. https://doi.org/10.1063/1.1715026
Cherns D, Hutchison JL, Jenkins ML, Hirsch PB, White S (1980) Electron irradiation induced vitrification at dislocations in quartz. Nature 287(5780):314–316. https://doi.org/10.1038/287314a0
Weichan C (1955) Electron microscopical study of extension processes by means of a spreading cartridge. Zeitschrift fur wissenschaftliche Mikroskopie und mikroskopische Technik 62(3):147–151
Wilsdorf H (1958) Apparatus for the deformation of foils in an electron microscope. Rev Sci Instrum 29(4):323–324. https://doi.org/10.1063/1.1716192
Takahashi N, Takeyama T, Ito K, Ito T, Mihama K, Watanabe M (1956) High temperature furnace for the electron microscope. J Electron Microsc 4(1):16–23. https://doi.org/10.1093/oxfordjournals.jmicro.a051220
Blech IA, Meieran ES (1967) Direct transmission electron microscope observation of electrotransport in aluminum thin films. Appl Phys Lett 11(8):263–266. https://doi.org/10.1063/1.1755127
Iwatsuki M, Murooka K, Kitamura S-i, Takayanagi K, Harada Y (1991) Scanning Tunneling Microscope (STM) for conventional Transmission Electron Microscope (TEM). J Electron Microsc 40(1):48–53. https://doi.org/10.1093/oxfordjournals.jmicro.a050869
Dong H, Xu T, Sun Z, Zhang Q, Wu X, He L, Xu F, Sun L (2018) Simultaneous atomic-level visualization and high precision photocurrent measurements on photoelectric devices by in situ TEM. RSC Adv 8(2):948–953. https://doi.org/10.1039/c7ra10696c
Suzuki K, Ichihara M, Takeuchi S, Nakagawa K, Maeda K, Iwanaga H (1984) In situ TEM observation of dislocation motion in II–VI compounds. Philos Mag A 49(3):451–461. https://doi.org/10.1080/01418618408233287
Zewail AH (2005) Diffraction, crystallography and microscopy beyond three dimensions: structural dynamics in space and time. Philos Trans Roy Soc A: Math Phys Eng Sci 363(1827):315–329. https://doi.org/10.1098/rsta.2004.1513
Hale ME, Fuller HW, Rubinstein H (1959) Magnetic domain observations by electron microscopy. J Appl Phys 30(5):789–791. https://doi.org/10.1063/1.1735233
Uhlig T, Heumann M, Zweck J (2003) Development of a specimen holder for in situ generation of pure in-plane magnetic fields in a transmission electron microscope. Ultramicroscopy 94(3):193–196. https://doi.org/10.1016/S0304-3991(02)00264-4
Zhang C, Firestein KL, Fernando JFS, Siriwardena D, von Treifeldt JE, Golberg D (2020) Recent progress of in situ transmission electron microscopy for energy materials. Adv Mater 32(18):1904094. https://doi.org/10.1002/adma.201904094
Xu T, Sun L (2016) Investigation on material behavior in liquid by in situ TEM. Superlattices Microstruct 99:24–34. https://doi.org/10.1016/j.spmi.2016.06.021
Xu T, Sun L (2015) Dynamic in-situ experimentation on nanomaterials at the atomic scale. Small 11(27):3247–3262. https://doi.org/10.1002/smll.201403236
Zheng H, Zhu Y (2017) Perspectives on in situ electron microscopy. Ultramicroscopy 180:188–196. https://doi.org/10.1016/j.ultramic.2017.03.022
Xu T, Shen Y, Yin K, Sun L (2019) Precisely monitoring and tailoring 2D nanostructures at the atomic scale. APL Mater 7(5):050901. https://doi.org/10.1063/1.5096584
Liu X, Xu T, Wu X, Zhang Z, Yu J, Qiu H, Hong J-H, Jin C-H, Li J-X, Wang X-R, Sun L-T, Guo W (2013) Top–down fabrication of sub-nanometre semiconducting nanoribbons derived from molybdenum disulfide sheets. Nat Commun 4:1776. https://doi.org/10.1038/ncomms2803
Shen Y, Xu T, Tan X, He L, Yin K, Wan N, Sun L (2018) In Situ Repair of 2D Chalcogenides under Electron Beam Irradiation. Adv Mater 30(14):1705954. https://doi.org/10.1002/adma.201705954
Krasheninnikov AV, Nordlund K (2010) Ion and electron irradiation-induced effects in nanostructured materials. J Appl Phys 107(7):071301. https://doi.org/10.1063/1.3318261
Xu T, Zhou Y, Tan X, Yin K, He L, Banhart F, Sun L (2017) Creating the smallest BN nanotube from Bilayer h-BN. Adv Func Mater 27(19):1603897. https://doi.org/10.1002/adfm.201603897
Tripathi M, Mittelberger A, Pike NA, Mangler C, Meyer JC, Verstraete MJ, Kotakoski J, Susi T (2018) Electron-beam manipulation of silicon dopants in graphene. Nano Lett 18(8):5319–5323. https://doi.org/10.1021/acs.nanolett.8b02406
Hinks JA (2009) A review of transmission electron microscopes with in situ ion irradiation. Nucl Instrum Methods Phys Res, Sect B 267(23):3652–3662. https://doi.org/10.1016/j.nimb.2009.09.014
Barwick B, Park HS, Kwon O-H, Baskin JS, Zewail AH (2008) 4D imaging of transient structures and morphologies in ultrafast electron microscopy. Science 322(5905):1227. https://doi.org/10.1126/science.1164000
Fu X, Chen B, Tang J, Hassan MT, Zewail AH (2017) Imaging rotational dynamics of nanoparticles in liquid by 4D electron microscopy. Science 355(6324):494. https://doi.org/10.1126/science.aah3582
Yoo B-K, Kwon O-H, Liu H, Tang J, Zewail AH (2015) Observing in space and time the ephemeral nucleation of liquid-to-crystal phase transitions. Nat Commun 6(1):8639. https://doi.org/10.1038/ncomms9639
Zhang M, Olson E, Twesten R, Wen J, Allen L, Robertson I, Petrov I (2005) In situ transmission electron microscopy studies enabled by microelectromechanical system technology. J Mater Res 20(7):1802–1807. https://doi.org/10.1557/JMR.2005.0225
Allard LF, Bigelow WC, Jose-Yacaman M, Nackashi DP, Damiano J, Mick SE (2009) A new MEMS-based system for ultra-high-resolution imaging at elevated temperatures. Microsc Res Tech 72(3):208–215. https://doi.org/10.1002/jemt.20673
Yu K, Zhao W, Wu X, Zhuang J, Hu X, Zhang Q, Sun J, Xu T, Chai Y, Ding F, Sun L (2018) In situ atomic-scale observation of monolayer graphene growth from SiC. Nano Res 11(5):2809–2820. https://doi.org/10.1007/s12274-017-1911-x
He L-B, Zhang L, Tan X-D, Tang L-P, Xu T, Zhou Y-L, Ren Z-Y, Wang Y, Teng C-Y, Sun L-T, Nie J-F (2017) Surface energy and surface stability of ag nanocrystals at elevated temperatures and their dominance in sublimation-induced shape evolution. Small 13(27):1700743. https://doi.org/10.1002/smll.201700743
Lian R, Yu H, He L, Zhang L, Zhou Y, Bu X, Xu T, Sun L (2016) Sublimation of Ag nanocrystals and their wetting behaviors with graphene and carbon nanotubes. Carbon 101:368–376. https://doi.org/10.1016/j.carbon.2016.01.105
He X, Xu T, Xu X, Zeng Y, Xu J, Sun L, Wang C, Xing H, Wu B, Lu A, Liu D, Chen X, Chu J (2014) In situ atom scale visualization of domain wall dynamics in VO2 insulator-metal phase transition. Sci Rep 4:6544. https://doi.org/10.1038/srep06544
Zhang Q, Yin K, Dong H, Zhou Y, Tan X, Yu K, Hu X, Xu T, Zhu C, Xia W, Xu F, Zheng H, Sun L (2017) Electrically driven cation exchange for in situ fabrication of individual nanostructures. Nat Commun 8:14889. https://doi.org/10.1038/ncomms14889
Wan N, Perriat P, Sun L, Huang Q, Sun J, Xu T (2012) Fullerene as electrical hinge. Appl Phys Lett 100(19):193111. https://doi.org/10.1063/1.4714682
Wan N, Sun L, Ding S, Xu T, Hu X, Sun J, Bi H (2013) Synthesis of graphene–CNT hybrids via joule heating: structural characterization and electrical transport. Carbon 53:260–268. https://doi.org/10.1016/j.carbon.2012.10.057
Huang JY, Zhong L, Wang CM, Sullivan JP, Xu W, Zhang LQ, Mao SX, Hudak NS, Liu XH, Subramanian A, Fan H, Qi L, Kushima A, Li J (2010) In situ observation of the electrochemical lithiation of a single SnO2 nanowire electrode. Science 330(6010):1515–1520. https://doi.org/10.1126/science.1195628
Xu F, Wu L, Meng Q, Kaltak M, Huang J, Durham JL, Fernandez-Serra M, Sun L, Marschilok AC, Takeuchi ES, Takeuchi KJ, Hybertsen MS, Zhu Y (2017) Visualization of lithium-ion transport and phase evolution within and between manganese oxide nanorods. Nat Commun 8(1):15400. https://doi.org/10.1038/ncomms15400
Wu X, Yu K, Cha D, Bosman M, Raghavan N, Zhang X, Li K, Liu Q, Sun L, Pey K (2018) Atomic scale modulation of self-rectifying resistive switching by interfacial defects. Adv Sci 5(6):1800096. https://doi.org/10.1002/advs.201800096
Liu Q, Sun J, Lv H, Long S, Yin K, Wan N, Li Y, Sun L, Liu M (2012) Real-time observation on dynamic growth/dissolution of conductive filaments in oxide-electrolyte-based ReRAM. Adv Mater 24(14):1844–1849. https://doi.org/10.1002/adma.201104104
Sun J, He L, Lo Y-C, Xu T, Bi H, Sun L, Zhang Z, Mao SX, Li J (2014) Liquid-like pseudoelasticity of sub-10-nm crystalline silver particles. Nat Mater 13(11):1007–1012. https://doi.org/10.1038/nmat4105
Zhong L, Sansoz F, He Y, Wang C, Zhang Z, Mao SX (2017) Slip-activated surface creep with room-temperature super-elongation in metallic nanocrystals. Nat Mater 16(4):439–445. https://doi.org/10.1038/nmat4813
Zheng H, Cao A, Weinberger CR, Huang JY, Du K, Wang J, Ma Y, Xia Y, Mao SX (2010) Discrete plasticity in sub-10-nm-sized gold crystals. Nat Commun 1(1):144. https://doi.org/10.1038/ncomms1149
Zhu Y, Espinosa HD (2005) An electromechanical material testing system for in situ electron microscopy and applications. Proc Natl Acad Sci 102(41):14503–14508. https://doi.org/10.1073/pnas.0506544102
Wang X, Zhong L, Mao SX (2018) Advances in understanding atomic-scale deformation of small-sized face-centered cubic metals with in situ transmission electron microscopy. Mater Today Nano 2:58–69. https://doi.org/10.1016/j.mtnano.2018.09.002
Yu Q, Legros M, Minor A (2015) In situ TEM nanomechanics. MRS Bull 40(1):62–70. https://doi.org/10.1557/mrs.2014.306
Spiecker E, Oh SH, Shan Z-W, Ikuhara Y, Mao SX (2019) Insights into fundamental deformation processes from advanced in situ transmission electron microscopy. MRS Bull 44(6):443–449. https://doi.org/10.1557/mrs.2019.129
Fernando JFS, Zhang C, Firestein KL, Golberg D (2017) Optical and optoelectronic property analysis of nanomaterials inside transmission electron microscope. Small 13(45):1701564. https://doi.org/10.1002/smll.201701564
Yang S, Wang L, Tian X, Xu Z, Wang W, Bai X, Wang E (2012) The piezotronic effect of zinc oxide nanowires studied by in situ TEM. Adv Mater 24(34):4676–4682. https://doi.org/10.1002/adma.201104420
Dong H, Xu F, Sun Z, Wu X, Zhang Q, Zhai Y, Tan XD, He L, Xu T, Zhang Z, Duan X, Sun L (2019) In situ interface engineering for probing the limit of quantum dot photovoltaic devices. Nat Nanotechnol 14(10):950–956. https://doi.org/10.1038/s41565-019-0526-7
Zheng H, Smith RK, Jun Y-w, Kisielowski C, Dahmen U, Alivisatos AP (2009) Observation of single colloidal platinum nanocrystal growth trajectories. Science 324(5932):1309–1312. https://doi.org/10.1126/science.1172104
Liao H-G, Zherebetskyy D, Xin H, Czarnik C, Ercius P, Elmlund H, Pan M, Wang L-W, Zheng H (2014) Facet development during platinum nanocube growth. Science 345(6199):916–919. https://doi.org/10.1126/science.1253149
Liao H-G, Cui L, Whitelam S, Zheng H (2012) Real-time imaging of Pt3Fe nanorod growth in solution. Science 336(6084):1011–1014
Zhou Y, Powers AS, Zhang X, Xu T, Bustillo K, Sun L, Zheng H (2017) Growth and assembly of cobalt oxide nanoparticle rings at liquid nanodroplets with solid junction. Nanoscale 9(37):13915–13921. https://doi.org/10.1039/c7nr04554a
Zeng Z, Zhang X, Bustillo K, Niu K, Gammer C, Xu J, Zheng H (2015) In situ study of lithiation and delithiation of MoS2 nanosheets using electrochemical liquid cell transmission electron microscopy. Nano Lett 15(8):5214–5220. https://doi.org/10.1021/acs.nanolett.5b02483
Niu K-Y, Park J, Zheng H, Alivisatos AP (2013) Revealing bismuth oxide hollow nanoparticle formation by the kirkendall effect. Nano Lett 13(11):5715–5719. https://doi.org/10.1021/nl4035362
Leenheer AJ, Sullivan JP, Shaw MJ, Harris CT (2015) A sealed liquid cell for in situ transmission electron microscopy of controlled electrochemical processes. J Microelectromech Syst 24(4):1061–1068. https://doi.org/10.1109/jmems.2014.2380771
Ye X, Jones MR, Frechette LB, Chen Q, Powers AS, Ercius P, Dunn G, Rotskoff GM, Nguyen SC, Adiga VP, Zettl A, Rabani E, Geissler PL, Alivisatos AP (2016) Single-particle mapping of nonequilibrium nanocrystal transformations. Science 354(6314):874. https://doi.org/10.1126/science.aah4434
Zhu C, Liang S, Song E, Zhou Y, Wang W, Shan F, Shi Y, Hao C, Yin K, Zhang T, Liu J, Zheng H, Sun L (2018) In-situ liquid cell transmission electron microscopy investigation on oriented attachment of gold nanoparticles. Nat Commun 9(1):421. https://doi.org/10.1038/s41467-018-02925-6
Yuk JM, Park J, Ercius P, Kim K, Hellebusch DJ, Crommie MF, Lee JY, Zettl A, Alivisatos AP (2012) High-resolution EM of colloidal nanocrystal growth using graphene liquid cells. Science 336(6077):61–64. https://doi.org/10.1126/science.1217654
Gai PL, Boyes ED (2009) Advances in atomic resolution in situ environmental transmission electron microscopy and 1Å aberration corrected in situ electron microscopy. Microsc Res Tech 72(3):153–164. https://doi.org/10.1002/jemt.20668
Luo L, Liu B, Song S, Xu W, Zhang J-G, Wang C (2017) Revealing the reaction mechanisms of Li–O2 batteries using environmental transmission electron microscopy. Nat Nanotechnol 12(6):535–539. https://doi.org/10.1038/nnano.2017.27
Crozier PA, Hansen TW (2015) In situ and operando transmission electron microscopy of catalytic materials. MRS Bull 40(1):38–45. https://doi.org/10.1557/mrs.2014.304
Yuan W, Zhu B, Li X-Y, Hansen TW, Ou Y, Fang K, Yang H, Zhang Z, Wagner JB, Gao Y, Wang Y (2020) Visualizing H2O molecules reacting at TiO2 active sites with transmission electron microscopy. Science 367(6476):428. https://doi.org/10.1126/science.aay2474
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Sun, L., Xu, T., Zhang, Z. (2023). Introduction to In-Situ Transmission Electron Microscopy. In: Sun, L., Xu, T., Zhang, Z. (eds) In-Situ Transmission Electron Microscopy. Springer, Singapore. https://doi.org/10.1007/978-981-19-6845-7_1
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