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Mouse Hematopoietic Stem Cell Transplantation

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Rodent Transplant Medicine

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Abstract

Since the first mouse model of HSC transplantation was established in the 1950s, decades of study have made mouse HSC transplantation become a well-established experimental method for investigating the function of HSCs in various conditions and confirming the curative potential of HSCs in hematological malignancies or disorders. In this chapter, we briefly summarize several important considerations in choosing the suitable donor, recipient, and HSC source before initiating a mouse HSC transplantation experiment.

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References

  • Adair JE, Zhao X, Chien S, Fang M, Wohlfahrt ME, Trobridge GD, Taylor JA, Beard BC, Kiem HP, Becker PS. Cyclophosphamide promotes engraftment of gene-modified cells in a mouse model of Fanconi anemia without causing cytogenetic abnormalities. J Mol Med (Berl). 2012;90(11):1283–94.

    Article  CAS  Google Scholar 

  • Bernitz JM, Daniel MG, Fstkchyan YS, Moore K. Granulocyte colony-stimulating factor mobilizes dormant hematopoietic stem cells without proliferation in mice. Blood. 2017;129(14):1901–12.

    Article  CAS  Google Scholar 

  • Broxmeyer HE, Orschell CM, Clapp DW, Hangoc G, Cooper S, Plett PA, Liles WC, Li X, Graham-Evans B, Campbell TB, Calandra G, Bridger G, Dale DC, Srour EF. Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med. 2005;201(8):1307–18.

    Article  CAS  Google Scholar 

  • Cao TM, Lo B, Ranheim EA, Grumet FC, Shizuru JA. Variable hematopoietic graft rejection and graft-versus-host disease in MHC-matched strains of mice. Proc Natl Acad Sci U S A. 2003;100(20):11571–6.

    Article  CAS  Google Scholar 

  • Chen J, Larochelle A, Fricker S, Bridger G, Dunbar CE, Abkowitz JL. Mobilization as a preparative regimen for hematopoietic stem cell transplantation. Blood. 2006;107(9):3764–71.

    Article  CAS  Google Scholar 

  • Cheng H, Liang PH, Cheng T. Mouse hematopoietic stem cell transplantation. Methods Mol Biol. 2013;976:25–35.

    Article  CAS  Google Scholar 

  • Chung B, Dudl E, Toyama A, Barsky L, Weinberg KI. Importance of interleukin-7 in the development of experimental graft-versus-host disease. Biol Blood Marrow Transplant. 2008;14(1):16–27.

    Article  CAS  Google Scholar 

  • Cooke KR, Kobzik L, Martin TR, Brewer J, Delmonte J Jr, Crawford JM, Ferrara JL. An experimental model of idiopathic pneumonia syndrome after bone marrow transplantation: I. The roles of minor H antigens and endotoxin. Blood. 1996;88(8):3230–9.

    Article  CAS  Google Scholar 

  • Cui YZ, Hisha H, Yang GX, Fan TX, Jin T, Li Q, Lian Z, Ikehara S. Optimal protocol for total body irradiation for allogeneic bone marrow transplantation in mice. Bone Marrow Transplant. 2002;30(12):843–9.

    Article  Google Scholar 

  • Czechowicz A, Palchaudhuri R, Scheck A, Hu Y, Hoggatt J, Saez B, Pang WW, Mansour MK, Tate TA, Chan YY, Walck E, Wernig G, Shizuru JA, Winau F, Scadden DT, Rossi DJ. Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation. Nat Commun. 2019;10(1):617.

    Article  CAS  Google Scholar 

  • Ford CE, Hamerton JL, Barnes DW, Loutit JF. Cytological identification of radiation-chimaeras. Nature. 1956;177(4506):452–4.

    Article  CAS  Google Scholar 

  • Garcia-Perez L, Van Roon L, Schilham MW, Lankester AC, Pike-Overzet K, Staal FJT. Combining mobilizing agents with Busulfan to reduce chemotherapy-based conditioning for hematopoietic stem cell transplantation. Cell. 2021;10(5):1077.

    Article  CAS  Google Scholar 

  • Grahn D, Hamilton KF. Genetic variation in the acute lethal response of four inbred mouse strains to whole body X-irradiation. Genetics. 1957;42(3):189–98.

    Article  CAS  Google Scholar 

  • Haas S, Hansson J, Klimmeck D, Loeffler D, Velten L, Uckelmann H, Wurzer S, Prendergast AM, Schnell A, Hexel K, Santarella-Mellwig R, Blaszkiewicz S, Kuck A, Geiger H, Milsom MD, Steinmetz LM, Schroeder T, Trumpp A, Krijgsveld J, Essers MA. Inflammation-induced emergency megakaryopoiesis driven by hematopoietic stem cell-like megakaryocyte progenitors. Cell Stem Cell. 2015;17(4):422–34.

    Article  CAS  Google Scholar 

  • Leins H, Mulaw M, Eiwen K, Sakk V, Liang Y, Denkinger M, Geiger H, Schirmbeck R. Aged murine hematopoietic stem cells drive aging-associated immune remodeling. Blood. 2018;132(6):565–76.

    Article  CAS  Google Scholar 

  • Lewis K, Yoshimoto M, Takebe T. Fetal liver hematopoiesis: from development to delivery. Stem Cell Res Ther. 2021;12(1):139.

    Article  Google Scholar 

  • Li Z, Lan Y, He W, Chen D, Wang J, Zhou F, Wang Y, Sun H, Chen X, Xu C, Li S, Pang Y, Zhang G, Yang L, Zhu L, Fan M, Shang A, Ju Z, Luo L, Ding Y, Guo W, Yuan W, Yang X, Liu B. Mouse embryonic head as a site for hematopoietic stem cell development. Cell Stem Cell. 2012;11(5):663–75.

    Article  CAS  Google Scholar 

  • Mazurier F, Doedens M, Gan OI, Dick JE. Rapid myeloerythroid repopulation after intrafemoral transplantation of NOD-SCID mice reveals a new class of human stem cells. Nat Med. 2003;9(7):959–63.

    Article  CAS  Google Scholar 

  • Morrison SJ, Wright DE, Weissman IL. Cyclophosphamide/granulocyte colony-stimulating factor induces hematopoietic stem cells to proliferate prior to mobilization. Proc Natl Acad Sci U S A. 1997;94(5):1908–13.

    Article  CAS  Google Scholar 

  • Nevozhay D, Opolski A. Key factors in experimental mouse hematopoietic stem cell transplantation. Arch Immunol Ther Exp. 2006;54(4):253–69.

    Article  Google Scholar 

  • Perry JM, Li L. Functional assays for hematopoietic stem cell self-renewal. Methods Mol Biol. 2010;636:45–54.

    Article  Google Scholar 

  • Reddy P, Negrin R, Hill GR. Mouse models of bone marrow transplantation. Biol Blood Marrow Transplant. 2008;14(1 Suppl 1):129–35.

    Article  Google Scholar 

  • Seita J, Weissman IL. Hematopoietic stem cell: self-renewal versus differentiation. Wiley Interdiscip Rev Syst Biol Med. 2010;2(6):640–53.

    Article  CAS  Google Scholar 

  • Skelton JK, Ortega-Prieto AM, Dorner M. A Hitchhiker’s guide to humanized mice: new pathways to studying viral infections. Immunology. 2018;154(1):50–61.

    Article  CAS  Google Scholar 

  • van Os R, Dawes D, Mislow JM, Witsell A, Mauch PM. Host conditioning with 5-fluorouracil and kit-ligand to provide for long-term bone marrow engraftment. Blood. 1997;89(7):2376–83.

    Article  Google Scholar 

  • Wilkinson AC, Nakauchi H. Stabilizing hematopoietic stem cells in vitro. Curr Opin Genet Dev. 2020;64:1–5.

    Article  CAS  Google Scholar 

  • Wilkinson AC, Igarashi KJ, Nakauchi H. Haematopoietic stem cell self-renewal in vivo and ex vivo. Nat Rev Genet. 2020;21(9):541–54.

    Article  CAS  Google Scholar 

  • Winkler IG, Pettit AR, Raggatt LJ, Jacobsen RN, Forristal CE, Barbier V, Nowlan B, Cisterne A, Bendall LJ, Sims NA, Levesque JP. Hematopoietic stem cell mobilizing agents G-CSF, cyclophosphamide or AMD3100 have distinct mechanisms of action on bone marrow HSC niches and bone formation. Leukemia. 2012;26(7):1594–601.

    Article  CAS  Google Scholar 

  • Xian Y, Xie Y, Song B, Ou Z, Ouyang S, Yang Y, Xiong Z, Li H, Sun X. The safety and effectiveness of genetically corrected iPSCs derived from beta-thalassaemia patients in nonmyeloablative beta-thalassaemic mice. Stem Cell Res Ther. 2020;11(1):288.

    Article  CAS  Google Scholar 

  • Yoder MC, Hiatt K. Engraftment of embryonic hematopoietic cells in conditioned newborn recipients. Blood. 1997;89(6):2176–83.

    Article  CAS  Google Scholar 

  • Youshani AS, Rowlston S, O'leary C, Forte G, Parker H, Liao A, Telfer B, Williams K, Kamaly-Asl ID, Bigger BW. Non-myeloablative busulfan chimeric mouse models are less pro-inflammatory than head-shielded irradiation for studying immune cell interactions in brain tumours. J Neuroinflammation. 2019;16(1):25.

    Article  Google Scholar 

  • Zhan Y, Zhao Y. Hematopoietic stem cell transplant in mice by intra-femoral injection. Methods Mol Biol. 2008;430:161–9.

    Article  Google Scholar 

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Authors and Affiliations

  1. Unit 4, Department of Hematology, The Fifth Medical Center of PLA General Hospital, Beijing, People’s Republic of China

    Shunzong Yuan, Bei Liu & Wenrong Huang

Authors
  1. Shunzong Yuan
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  2. Bei Liu
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  3. Wenrong Huang
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Editors and Affiliations

  1. Department of Surgery, Zhejiang University School of Medicine, Hangzhou, China

    Weihua Gong

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Yuan, S., Liu, B., Huang, W. (2022). Mouse Hematopoietic Stem Cell Transplantation. In: Gong, W. (eds) Rodent Transplant Medicine. Springer, Singapore. https://doi.org/10.1007/978-981-19-6111-3_17

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  • DOI: https://doi.org/10.1007/978-981-19-6111-3_17

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-19-6110-6

  • Online ISBN: 978-981-19-6111-3

  • eBook Packages: MedicineMedicine (R0)

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