Abstract
Stem cell harvest is a procedure that involves collecting the stem cells from either host for autologous stem cell transplant (SCT) or from the donor for allogeneic SCT. Harvest can be done either from bone marrow or from peripheral blood. Peripheral blood stem cell (PBSC) collection may allow donors to avoid the risk of anesthesia (general or spinal), hospital admission, and potential exposure to blood products. Engraftment is earlier with PBSC graft compared to bone marrow graft. The procedure requires collaboration with anesthesiologists and blood bank physicians.
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References
Padmanabhan A, Connelly-Smith L, Aqui N, Balogun RA, Klingel R, Meyer E, et al. Guidelines on the use of therapeutic apheresis in clinical practice—evidence-based approach from the writing committee of the American society for apheresis: the eighth special issue. J Clin Apher. 2019;34(3):171–354.
Burns LJ, Logan BR, Chitphakdithai P, Miller JP, Drexler R, Spellman S, et al. Recovery of unrelated donors of peripheral blood stem cells versus recovery of unrelated donors of bone marrow: a prespecified analysis from the phase III blood and marrow transplant clinical trials network protocol 0201. Biol Blood Marrow Transplant. 2016;22(6):1108–16.
Pulsipher MA, Chitphakdithai P, Logan BR, Navarro WH, Levine JE, Miller JP, et al. Lower risk for serious adverse events and no increased risk for cancer after PBSC vs BM donation. Blood. 2014;123(23):3655–63.
Heldal D, Brinch L, Tjønnfjord G, Solheim BG, Egeland T, Gadeholt G, et al. Donation of stem cells from blood or bone marrow: results of a randomised study of safety and complaints. Bone Marrow Transplant. 2002;29(6):479–86.
Miller JP, Perry EH, Price TH, Bolan CD, Karanes C, Boyd TM, et al. Recovery and safety profiles of marrow and PBSC donors: experience of the National marrow donor program. Biol Blood Marrow Transplant. 2008;14(9, Supplement):29–36.
DiPersio JF, Khoury H, Haug J, Vij R, Adkins DR, Goodnough LT, et al. Innovations in allogeneic stem-cell transplantation. Semin Hematol. 2000;37:33–41.
Cottler-Fox MH, Lapidot T, Petit I, Kollet O, DiPersio JF, Link D, et al. Stem cell mobilization. Hematol Am Soc Hematol Educ Program. 2003;2003:419–37.
Cassens U, Momkvist PH, Zuehlsdorf M, Mohr M, Kienast J, Berdel WE, et al. Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC). Bone Marrow Transplant. 2001;28(1):13–20.
Stroncek DF, Clay ME, Herr G, Smith J, Jaszcz WB, Ilstrup S, et al. The kinetics of G-CSF mobilization of CD34+ cells in healthy people. Transfus Med. 1997;7(1):19–24.
Howell C, Douglas K, Cho G, El-Ghariani K, Taylor P, Potok D, et al. Guideline on the clinical use of apheresis procedures for the treatment of patients and collection of cellular therapy products. British committee for standards in haematology. Transfus Med. 2015;25(2):57–78.
Moncada V, Bolan C, Yau YY, Leitman SF. Analysis of PBPC cell yields during large-volume leukapheresis of subjects with a poor mobilization response to filgrastim. Transfusion (Paris). 2003;43(4):495–501.
Morstyn G, Campbell L, Dührsen U, Souza LM, Alton NK, Villeval JL, et al. Clinical studies with granulocyte colony stimulating factor (G-CSF) in patients receiving cytotoxic chemotherapy. Behring Inst Mitt. 1988;83:234–9.
Hequet O. Hematopoietic stem and progenitor cell harvesting: technical advances and clinical utility. J Blood Med. 2015;6:55–67.
Möhle R, Murea S, Pförsich M, Witt B, Haas R. Estimation of the progenitor cell yield in a leukapheresis product by previous measurement of CD34+ cells in the peripheral blood. Vox Sang. 1996;71(2):90–6.
Root RK, Dale DC. Granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor: comparisons and potential for use in the treatment of infections in nonneutropenic patients. J Infect Dis. 1999;179(Supplement_2):S342–52.
Lionne-Huyghe P, Kuhnowski F, Coiteux V, Bauters F, Morschhauser F. Indications of G-CSF administration in hematologic disorders. Bull Cancer. 2006;93(5):453–62.
Cottler-Fox MH, Lapidot T, Petit I, Kollet O, DiPersio JF, Link D, et al. Stem cell mobilization. Hematology. 2003;2003(1):419–37.
Lapidot T, Petit I. Current understanding of stem cell mobilization: the roles of chemokines, proteolytic enzymes, adhesion molecules, cytokines, and stromal cells. Exp Hematol. 2002;30(9):973–81.
Heyns AD, Badenhorst PN, Lötter MG, Pieters H, Wessels P. Kinetics and mobilization from the spleen of indium-111-labeled platelets during platelet apheresis. Transfusion (Paris). 1985;25(3):215–8.
Dettke M, Hlousek M, Kurz M, Leitner G, Rosskopf K, Stiegler G, et al. Increase in endogenous thrombopoietin in healthy donors after automated plateletpheresis. Transfusion (Paris). 1998;38(5):449–53.
Lee G, Arepally GM. Anticoagulation techniques in apheresis: from heparin to citrate and beyond. J Clin Apher. 2012;27(3):117–25.
Strauss RG. Mechanisms of adverse effects during hemapheresis. J Clin Apher. 1996;11(3):160–4.
Silberstein LE, Naryshkin S, Haddad JJ, Strauss JF. Calcium homeostasis during therapeutic plasma exchange. Transfusion (Paris). 1986;26(2):151–5.
Uhl L, Maillet S, King S, Kruskall MS. Unexpected citrate toxicity and severe hypocalcemia during apheresis. Transfusion (Paris). 1997;37(10):1063–5.
Winters JL. Complications of donor apheresis. J Clin Apher. 2006;21(2):132–41.
Urban P, Scheidegger D, Buchmann B, Barth D. Cardiac arrest and blood ionized calcium levels. Ann Intern Med. 1988;109(2):110–3.
Pepe J, Colangelo L, Biamonte F, Sonato C, Danese VC, Cecchetti V, et al. Diagnosis and management of hypocalcemia. Endocrine. 2020;69(3):485–95.
Bunker JP, Bendixen HH, Murphy AJ. Hemodynamic effects of intravenously administered sodium citrate. N Engl J Med. 1962;266(8):372–7.
Antonic M, Gubensek J, Buturović-Ponikvar J, Ponikvar R. Comparison of citrate anticoagulation during plasma exchange with different replacement solutions. Ther Apher Dial. 2009;13(4):322–6.
Weinstein R. Prevention of citrate reactions during therapeutic plasma exchange by constant infusion of calcium gluconate with the return fluid. J Clin Apher. 1996;11(4):204–10.
Broner CW, Stidham GL, Westenkirchner DF, Watson DC. A prospective, randomized, double-blind comparison of calcium chloride and calcium gluconate therapies for hypocalcemia in critically ill children. J Pediatr. 1990;117(6):986–9.
Owen HG, Brecher ME. Atypical reactions associated with use of angiotensin-converting enzyme inhibitors and apheresis. Transfusion (Paris). 1994;34(10):891–4.
Bolan CD, Greer SE, Cecco SA, Oblitas JM, Rehak NN, Leitman SF. Comprehensive analysis of citrate effects during plateletpheresis in normal donors. Transfusion (Paris). 2001;41(9):1165–71.
Mercan D, Bastin G, Lambermont M, Dupont E. Importance of ionized magnesium measurement for monitoring of citrate-anticoagulated plateletpheresis. Transfusion (Paris). 1997;37(4):418–22.
Hansen BA, Bruserud Ø. Hypomagnesemia in critically ill patients. J Intensive Care. 2018;6(1):21.
Olson PR, Cox C, McCullough J. Laboratory and clinical effects of the infusion of ACD solution during plateletpheresis. Vox Sang. 1977;33(2):79–87.
Whang R, Whang DD, Ryan MP. Refractory potassium repletion. A consequence of magnesium deficiency. Arch Intern Med. 1992;152(1):40–5.
Paulson K, Gilpin SG, Shpiruk TA, Anjos K, Tulloch M, Giftakis A, et al. Routine filtration of hematopoietic stem cell products: the time has arrived. Transfusion (Paris). 2015;55(8):1980–4.
Rowley SD, Anderson GL. Effect of DMSO exposure without cryopreservation on hematopoietic progenitor cells. Bone Marrow Transplant. 1993;11(5):389–93.
Leemhuis T, Padley D, Keever-Taylor C, Niederwieser D, Teshima T, Lanza F, et al. Essential requirements for setting up a stem cell processing laboratory. Bone Marrow Transplant. 2014;49(8):1098–105.
Bacigalupo A, Tong J, Podesta M, Piaggio G, Figari O, Colombo P, et al. Bone marrow harvest for marrow transplantation: effect of multiple small (2 ml) or large (20 ml) aspirates. Bone Marrow Transplant. 1992;9(6):467–70.
Batinić D, Marusić M, Pavletić Z, Bogdanić V, Uzarević B, Nemet D, et al. Relationship between differing volumes of bone marrow aspirates and their cellular composition. Bone Marrow Transplant. 1990;6(2):103–7.
Remberger M, Ringdén O, Mattsson J. Bone marrow aspiration technique has deteriorated in recent years. Bone Marrow Transplant. 2015;50(7):1007–9.
Keeney M, Chin-Yee I, Weir K, Popma J, Nayar R, Sutherland DR. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International society of hematotherapy and graft engineering. Cytometry. 1998;34(2):61–70.
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Sharma, S.K. (2023). Apheresis. In: Basics of Hematopoietic Stem Cell Transplant. Springer, Singapore. https://doi.org/10.1007/978-981-19-5802-1_14
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DOI: https://doi.org/10.1007/978-981-19-5802-1_14
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