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Preparation and Characterization of Polymer Biocomposite 3D Mat for Bone Tissue Regeneration

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Polymeric Biomaterials and Bioengineering

Part of the book series: Lecture Notes in Bioengineering ((LNBE))

Abstract

Bone scaffold is a three-dimensional matrix which stimulates the attachment and proliferation of osteoinducible cells on its surface. Use of scaffolds is the standard procedure to treat skeletal fractals, replace or regenerate the damaged/lost bone tissues. However, reproducing the complete features of the bone morphology, strength and porosity is a challenging task. The present study endeavors toward the preparation and bioactive studies of PCL/bioactive glass electrospun three-dimensional (3D) mat. The 3D mat was fabricated using eletrospinning process. To prepare biocomposites, 45S5 bioglass (1 wt%) prepared by sol–gel method was mixed with PCL (8 wt%) using chloroform and ethanol as a solvent, which has dispersibility and spinnability. The physicochemical characterization using X-ray diffraction and Fourier transform infrared spectroscopy confirmed the presence of bioglass in the biocomposite fibers. The scaffold structure was evaluated using scanning electron microscopy, which reveals the porous nature with an average fiber diameter of 116 ± 40 nm. In vitro studies revealed the mineralization ability of the prepared fibrous 3D mat in stimulated body fluid (SBF) under static conditions. The biocomposite scaffold exhibits higher calcium phosphate formation and higher degradation rate. These results indicate that PCL/BG biocomposite scaffolds as a favorable substance for bone tissue regeneration applications

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References

  1. Li H, Huang C, Jin X, Ke Q (2018) An electrospun poly(ε-caprolactone) nanocomposite fibrous mat with a high content of hydroxyapatite to promote cell infiltration. RSC Adv 8(44):25228–25235. https://doi.org/10.1039/C8RA02059K

    Article  CAS  Google Scholar 

  2. Yu Y, Yang M, Hua S, Fu Z, Teng S, Niu K, Zhao Q, Yi C (2016) Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold. RSC Adv 6(112):110557–110565. https://doi.org/10.1039/C6RA17718B

  3. Wang Q, Xu J, Jin H, Zheng W, Zhang X, Huang Y, Qian Z (2017) Artificial periosteum in bone defect repair—a review. Chinese Chem Lett 28(9):1801–180710. https://doi.org/10.1016/j.cclet.2017.07.011

  4. Fiume E, Barberi J, Verné E, Baino F (2018) Bioactive glasses: from parent 45S5 composition to scaffold-assisted tissue-healing therapies. J Funct Biomater 9(1).https://doi.org/10.3390/jfb9010024

  5. Hench LL (1998) Biomaterials: a forecast for the future. Biomaterials 19(16):1419–1423. https://doi.org/10.1016/s0142-9612(98)00133-1

    Article  CAS  Google Scholar 

  6. Petretta M, Gambardella A, Boi M, Berni M, Cavallo C, Marchiori G (2021) Composite scaffolds for bone tissue regeneration based on pcl and mg-containing bioactive glasses. Biology (Basel) 10(5):1–18. https://doi.org/10.3390/biology10050398

    Article  CAS  Google Scholar 

  7. Kolan K, Li W, Day DE, Ming CL (2016) 3D printing of a polymer bioactive glass composite for bone repair. In: Solid freeform fabrication 2016 proceeding 27th annual international solid freeform fabrication symposium—an additive manufacturing conference SFF 2016, no. January, pp 1718–1731

    Google Scholar 

  8. Fathi A, Kermani F, Behnamghader A, Banijamali S, Mozafari M, Baino F, Kargozar S (2021) Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering. Biomed Glas 6(1):57–69. https://doi.org/10.1515/bglass-2020-0006

  9. Asmaa MAEA, Ahemed AEF, Azza EM, Doaa AG, Kandil S (2021) Viscoelasticity, mechanical properties, and in vitro bioactivity of gelatin/borosilicate bioactive glass nanocomposite hydrogels as potential scaffolds for bone regeneration. Polymers (Basel) 13(12):2014. https://doi.org/10.3390/polym13122014

    Article  CAS  Google Scholar 

  10. Soni R, Vijay KN, Chameettachal S, Pati F, Rath SN (2019) Synthesis and optimization of PCL-bioactive glass composite scaffold for bone tissue engineering. Mater Today Proc 15:294–299

    Article  CAS  Google Scholar 

  11. Shivalingam C, Purushothaman B, Subramanian B (2020) Effect of microwave and probe sonication processes on sol–gel-derived bioactive glass and its structural and biocompatible investigations. J Biomed Mater Res Part B Appl Biomater 108(1):143–155. https://doi.org/10.1002/jbm.b.34373

  12. Hong W, Guo F, Hu L, Wang X, Xing C, Tan Y, Zhao X, Xiao P (2019) A hierarchically porous bioactive glass-ceramic microsphere with enhanced bioactivity for bone tissue engineering. Ceram Int 45(10):13579–13583. https://doi.org/10.1016/j.ceramint.2019.03.241

  13. Henrique LT, Gabriella MFC, Carlos EDMJ, Rodrigo LO, Armando da SCJ, Min Zhao, Francine BC and Gisele RDS (2016) Bioactive glass nanoparticles-loaded poly(ϵ-caprolactone) nanofiber as substrate for ARPE-19 cells. J Nanomater 2016. https://doi.org/10.1155/2016/4360659

  14. Bargavi P, Shivalingam C, Purushothaman B, Subramanian B (2020) Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications. Biomater Sci 8(14):4003–4025. https://doi.org/10.1039/d0bm00714e

  15. Shahin-SA AH, Tahriri M, Bastami F, Salehi M, Mashhadi AF (2018) Mechanical, material, and biological study of a PCL/bioactive glass bone scaffold: Importance of viscoelasticity. Mater Sci Eng C 90(April):280–288. https://doi.org/10.1016/j.msec.2018.04.080

  16. Izabella Rajzer MD, Kurowska A, Katarzyna CK, Ziabka M, Menszek E, Timothy ELD (2019) Electrospun polycaprolactone membranes with Zn-doped bioglass for nasal tissues treatment. J Mater Sci Mater Med 30(7). https://doi.org/10.1007/s10856-019-6280-4

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Acknowledgements

The authors are thankful to the Department of Science and Technology (DST) Science of Equity Empowerment and Development (SEED), CIPET:IPT, Chennai, and Material Development Lab, CIPET:SARP-ARSTPS, Chennai, for providing the necessary supports to carry out above research work.

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Ramar, G., Periyasamy, B.K., Joseph Bensingh, R., Nayak, S.K. (2022). Preparation and Characterization of Polymer Biocomposite 3D Mat for Bone Tissue Regeneration. In: Gupta, B., Jawaid, M., Kaith, B.S., Rattan, S., Kalia, S. (eds) Polymeric Biomaterials and Bioengineering. Lecture Notes in Bioengineering. Springer, Singapore. https://doi.org/10.1007/978-981-19-1084-5_2

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