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Molecular Identification of Insect Pests of Horticultural Crops

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Trends in Horticultural Entomology

Abstract

Accurate identification of species is fundamental to both basic and applied research. Classification and identification of various life forms, particularly insects, has been a major challenge to the scientific community with the dwindling interest in taxonomy and fund availability. In quarantine and plant protection activities, their immature stages are met with and diagnosis of these is important to foster a rapid, accurate species identification that is crucial in various spheres of pest management like biological control, insecticide resistance management, preventing the entry of invasive and alien species, and insect vector management that include identification of biotypes, cryptic species. With the advent of molecular biology and molecular tools, identification of life forms including insects has become quick, precise, and easy. Deoxyribonucleic acid (DNA) barcoding is an alternative way to accurately identify species, which also complements conventional taxonomy. DNA barcoding enables even a non-specialist to identify a species even using immature stages like egg, larva, nymph, or pupa. The mitochondrial cytochrome c oxidase subunit I (mtCO-I) region marker was used in the species diagnosis and genetic diversity research. The polymerase chain reaction (PCR) method developed effectively identified biotypes of insect pests. Molecular identification is applied to a great extent in sucking pests including thrips, mealybugs, whiteflies, aphids, and leafhoppers, besides fruit flies.

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  1. ICAR-Indian Institute of Horticultural Research, Bengaluru, India

    M. Mani

  2. ICAR-National Bureau of Agricultural Insect Resources, Bengaluru, India

    T. Venkatesan & B. R. Chethan

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    M. Mani

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Mani, M., Venkatesan, T., Chethan, B.R. (2022). Molecular Identification of Insect Pests of Horticultural Crops. In: Mani, M. (eds) Trends in Horticultural Entomology . Springer, Singapore. https://doi.org/10.1007/978-981-19-0343-4_1

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