Fish Diversity of Estuaries Revealed by Environmental DNA

Kume, Manabu; Lavergne, Edouard

doi:10.1007/978-981-16-7427-3_9

Manabu Kume⁴ &
Edouard Lavergne⁵^nAff6

710 Accesses
1 Altmetric

Abstract

Estuarine ecosystems are important habitats for many fish species. Large-scale patterns of estuarine fish communities can be affected by geographical factors, such as water temperature and latitude, which either limit or allow fish dispersal, and/or by environmental factors that select locally adapted species. Furthermore, increased human activity has also altered fish diversity through the modification of estuarine environments. In this study, we used environmental DNA metabarcoding to study a large-scale pattern of estuarine fish communities across 26 watersheds in the Japanese Archipelago, and assessed the effects of geographical, environmental, and anthropogenic factors in structuring these communities. Of the 197 fish species detected, 40 species were red-listed by the Japanese Ministry of Environment and seven were alien. Non-metric multidimensional scaling showed that both latitude and water temperature affected river mouth fish communities. Although other anthropogenic factors were not found to affect these communities, the bank artificialization rate was influenced. Furthermore, the relationship between the fish community and ocean currents was weak. The number of red-listed fish affected fish community structures, whereas that of alien species did not. The numbers of red-listed fish were negatively correlated with the bank artificialization rate and the rate of tetrapod formation. These findings provide useful information that may be used to conserve estuarine ecosystems.

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Abbreviations

BAR :: Bank artificialization rate
DO :: Dissolved oxygen
eDNA:: Environmental DNA
NMDS :: Non-metric multidimensional scaling
SR:: Species richness
TFR :: Tetrapod formation rate
TN :: Total nitrogen
WT :: Water temperature

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Acknowledgments

We would like to thank Yoh Yamashita, Akihide Kasai, Hyojin. Ahn, Kohmei Kadowaki, Yoshiaki Kai, Yumi Henmi, and many other colleagues for their intensive discussions. This study was conducted under the Link Again Program, part of the Connectivity of Hills, Humans and Oceans project, funded by the Nippon Foundation.

Author information

Edouard Lavergne
Present address: Université de Bretagne Occidentale, Plouzané, France

Authors and Affiliations

Field Science Education and Research Center, Kyoto University, Sakyo, Kyoto, Japan
Manabu Kume
Research and Educational Unit for Studies on Connectivity of Hills, Humans and Oceans, Kyoto University, Kyoto, Japan
Edouard Lavergne

Authors

Manabu Kume
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Edouard Lavergne
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Corresponding author

Correspondence to Manabu Kume .

Editor information

Editors and Affiliations

Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University, Maizuru, Kyoto, Japan
Yoshiaki Kai
The Kagoshima University Museum, Kagoshima, Kagoshima, Japan
Hiroyuki Motomura
Department of Zoology, National Museum of Nature and Science, Tsukuba, Ibaraki, Japan
Keiichi Matsuura

Appendix

List of fishes from 26 estuaries in Japan using environmental DNA metabarcoding (presence = 1/absence = 0). The river IDs are shown in Table 9.1. R* indicates red-list categories (CR, EN, NT, VU, LP, and DD) set by the Ministry of Environment (2017, 2019) and “AL” represents alien species

Scientific name	R*	River IDs
Scientific name	R*	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26
Lethenteron sp. S	VU	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hemitrygon akajei		0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
Aetobatus narutobiei		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0
Anguilla japonica	EN	1	0	0	0	1	1	1	1	0	0	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1
Ophisurus macrorhynchos		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Coilia nasus	EN	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Engraulis japonicus		0	0	1	0	1	1	1	1	1	1	0	0	0	0	0	1	0	0	1	1	1	0	1	0	0	1
Clupea pallasii	LP	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Etrumeus micropus		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0
Ilisha elongata		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Konosirus punctatus		1	1	1	0	1	1	1	1	1	1	1	0	0	1	0	1	1	1	1	1	0	1	1	1	1	1
Sardinella zunasi		0	0	0	0	1	1	0	0	1	1	0	0	0	0	0	1	1	0	1	0	1	0	1	0	1	0
Sardinops melanostictus		1	0	0	0	1	1	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
Spratelloides gracilis		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1
Cyprinus spp.		1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1
Acheilognathus cyanostigma	CR	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Acheilognathus melanogaster	EN	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Acheilognathus tabira jordani	CR	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Biwia zezera	VU	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Carassius spp.		1	1	1	1	1	0	1	0	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Ctenopharyngodon idella	AL	0	0	0	0	0	0	1	0	0	0	0	0	1	0	1	0	1	0	0	0	0	0	0	0	0	0
Gnathopogon caerulescens	CR	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Gnathopogon elongatus elongatus		0	1	1	0	0	1	0	0	1	0	1	0	1	1	0	0	0	0	0	0	0	1	0	0	0	0
Hemibarbus spp.		1	1	1	1	1	1	1	0	1	1	0	0	1	1	1	1	0	1	1	1	1	1	0	1	1	0
Nipponocypris temminckii		0	0	0	0	0	0	0	0	1	0	1	1	1	1	0	0	0	0	1	0	1	1	0	1	0	1
Rhynchocypris lagowskii steindachneri		1	1	1	1	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Rhynchocypris oxycephala		0	0	1	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pseudogobio esocinus		0	0	0	0	0	0	0	0	1	0	1	1	1	1	1	0	0	0	1	0	1	1	1	1	1	0
Pseudogobio agathonectris		0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	1	0	0	0	0	0	0	0
Pseudogobio polystictus		1	1	1	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pseudorasbora parva		1	1	1	1	0	0	0	1	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0
Pungtungia herzi		0	0	0	0	0	0	1	0	1	0	1	1	0	0	0	0	0	1	1	0	1	0	0	0	0	0
Rhodeus ocellatus ocellatus	AL	0	0	1	0	0	1	0	0	1	0	0	0	0	1	0	0	0	0	0	0	1	1	0	0	0	0
Sarcocheilichthys variegatus microoculus		1	1	1	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Squalidus gracilis gracilis		0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0
Squalius spp.	VU	0	0	0	0	0	0	1	0	0	0	0	0	1	0	1	0	1	1	1	0	1	1	0	0	0	0
Tanakia lanceolata	NT	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	1	0	0	0	0	0
Tanakia limbata	NT	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	1	0	0	0	0	0	0	0	0
Pseudaspius brandtii maruta		0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pseudaspius brandtii brandtii	LP	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pseudaspius hakonensis		1	1	1	1	0	0	1	1	1	1	1	1	1	1	0	0	0	0	1	1	1	1	1	1	1	0
Pseudaspius sachalinensis	LP	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Opsariichthys platypus		0	1	1	1	0	0	1	0	0	0	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1	1
Cobitis minamorii saninensis	EN	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cobitis sp. BIWAE typeA		0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cobitis sp. BIWAE typeB		0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cobitis sp. BIWAE typeC		0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cobitis minamorii tokaiensis		0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0
Cobitis striata striata	VU	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
Cobitis takatsuensis	EN	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Misgurnus anguillicaudatus	NT	1	1	1	1	1	0	0	0	1	1	1	1	1	0	0	0	0	0	0	0	1	1	0	0	0	0
Misgurnus sp. Clade A	DD	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Paramisgurnus dabryanus		0	1	0	0	1	0	0	0	0	1	1	0	1	0	1	1	0	0	0	0	0	1	0	0	0	0
Niwaella delicata	VU	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Barbatula oreas		1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Lefua echigonia	EN	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Liobagrus reinii	VU	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Silurus asotus		1	0	1	1	0	0	0	0	0	1	1	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0
Plotosus japonicus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
Tachysurus nudiceps		0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hypomesus olidus	NT	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Plecoglossus altivelis altivelis		1	0	0	1	0	0	1	1	1	0	1	1	1	1	1	0	0	1	1	0	1	1	1	1	1	0
Salangichthys ishikawae		0	0	0	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Salanx ariakensis	CR	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Oncorhynchus gorbuscha		1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Oncorhynchus masou masou	NT	1	1	1	1	0	0	0	0	1	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Oncorhynchus mykiss	AL	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Salvelinus spp.		0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Saurida elongata		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Diaphus chrysorhynchus		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Gadus macrocephalus		0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Chelon affinis		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	1	1
Chelon haematocheila		1	1	1	1	1	0	0	0	1	1	1	1	0	0	0	1	1	1	1	0	0	0	0	0	0	0
Chelon macrolepis		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
Mugil cephalus		1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Hypoatherina tsurugae		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
Hypoatherina valenciennei		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	1	1	0	0
Oryzias sakaizumii	VU	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cypselurus spp.		0	0	0	0	0	1	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Hyporhamphus intermedius	NT	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hyporhamphus sajori		0	0	0	1	1	1	0	0	1	1	1	0	0	1	0	1	1	0	0	0	1	0	1	0	0	0
Ablennes hians		0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Strongylura anastomella		0	0	1	1	1	1	0	1	1	1	0	0	0	1	0	1	0	0	1	0	0	0	1	0	1	1
Tylosurus crocodilus crocodilus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	1	0	0	0	0	1
Pungitius spp.	LP	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cololabis saira		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
Inimicus japonicus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Hypodytes rubripinnis		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Sebastes spp.		0	0	0	0	0	1	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0	1	0	0	0	0
Sebastiscus marmoratus		0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Chelidonichthys spinosus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Platycephalus sp. 1		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Platycephalus sp. 2		0	0	0	0	1	1	0	0	0	1	0	0	0	0	0	0	0	0	0	1	1	0	1	0	1	0
Hexagrammos spp.		0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	1	0	0
Rheopresbe kazika	VU	0	0	1	1	0	0	0	1	0	0	1	1	0	1	0	0	0	0	0	0	1	0	0	0	0	0
Cottus pollux	NT	0	1	1	1	0	0	0	0	1	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0
Cottus reinii	EN	1	1	0	0	0	0	0	1	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0
Lateolabrax japonicus	LP	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Lateolabrax latus		0	0	0	0	1	1	1	1	1	0	1	0	0	1	0	1	0	0	0	0	1	1	1	1	1	1
Lateolabrax maculatus	AL	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	1	1
Coreoperca kawamebari	EN	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
Acropoma japonicum		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Epinephelus areolatus		0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Epinephelus awoara		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1
Epinephelus merra		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
Lepomis macrochirus	AL	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0
Micropterus dolomieu dolomieu	AL	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Micropterus salmoides salmoides	AL	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	1	0	0	0	0	0
Apogon unicolor		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Sillago japonica		0	0	1	0	0	1	1	1	0	1	0	1	0	0	0	1	0	0	0	1	1	1	0	0	0	1
Seriola dumerili		0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	1
Seriola lalandi		0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Seriola quinqueradiata		0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	1	1	0	1	0	0	0	0
Trachinotus baillonii		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Trachurus japonicus		0	0	1	0	0	1	1	0	1	1	1	0	0	0	1	1	0	0	0	1	1	1	1	1	0	1
Equulites rivulatus		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuchequula nuchalis		1	0	1	0	0	0	0	0	1	1	0	0	0	0	0	1	1	1	1	1	0	0	0	1	1	0
Pterocaesio tile		0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Hapalogenys nigripinnis		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
Parapristipoma trilineatum		0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	1	0	0	1	0	0	1	0	0	0	0
Plectorhinchus cinctus		0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	1
Acanthopagrus latus		0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	1	1	0	1	1	1	1	1	1	1	1
Acanthopagrus schlegelii		0	1	1	0	1	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1	1	1	1
Evynnis tumifrons		0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0
Pagrus major		1	0	1	0	0	0	0	0	0	0	1	0	1	0	1	1	0	0	1	0	0	1	0	0	0	1
Rhabdosargus sarba		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0
Nibea albiflora	EN	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nibea mitsukurii		0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pennahia argentata		0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Girella punctata		0	0	1	0	1	1	1	0	0	1	0	0	0	1	0	1	0	0	0	1	0	1	0	0	0	1
Microcanthus strigatus		0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Rhynchopelates oxyrhynchus		0	0	0	0	0	1	1	0	0	1	0	0	0	0	0	1	0	1	0	1	1	1	0	1	0	1
Terapon jarbua		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
Oplegnathus fasciatus		0	0	0	0	0	0	1	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0
Acanthocepola krusensternii		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Ditrema temminckii subspp.		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	1	0	0	1	0	1	0	0
Halichoeres tenuispinis		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pseudolabrus sieboldi		0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Semicossyphus reticulatus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Stethojulis interrupta terina		0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
Dictyosoma burgeri Form a		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Dictyosoma burgeri Form b		0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
Dictyosoma rubrimaculatum		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Stichaeopsis nana		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Pholis spp.		0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Pholis nebulosa		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Entomacrodus stellifer stellifer		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Omobranchus fasciolatoceps		0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	1	0	0	0	0	1	0	0	1
Omobranchus punctatus		0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	1	1	1	1	0	0	1	1	1	1
Parablennius yatabei		0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	1
Repomucenus valenciennei		1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Odontobutis hikimius	VU	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Odontobutis obscura		0	0	0	0	0	0	0	0	1	0	1	1	0	0	0	0	0	1	1	0	1	1	1	1	0	0
Eleotris oxycephala		0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Acanthogobius flavimanus		1	1	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1	1	0	1	1	1	0
Acanthogobius hasta	VU	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Acentrogobius spp.		0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1
Amblychaeturichthys sciistius	NT	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Boleophthalmus pectinirostris	EN	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Callogobius tanegasimae		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0
Chaenogobius annularis		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Eutaeniichthys gilli	NT	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	1	0
Favonigobius gymnauchen		0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Acanthogobius lactipes		1	1	0	1	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	0	1	1	0
Glossogobius olivaceus		0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	1	0	0	0	0	0	0	1	1	1
Gymnogobius breunigii		1	1	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	1	0	1	1	0	1	0
Gymnogobius castaneus		0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Gymnogobius opperiens		1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Gymnogobius petschiliensis	LP	0	0	1	1	0	0	0	1	0	1	1	1	0	0	0	0	0	0	1	0	1	1	0	0	0	0
Gymnogobius scrobiculatus	EN	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	1	0	0	1	0
Gymnogobius urotaenia		1	1	1	1	0	0	1	0	0	0	1	1	1	0	1	0	0	1	0	0	0	1	1	0	0	0
Leucopsarion petersii	VU	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
Luciogobius guttatus		0	0	1	0	1	1	1	1	1	0	0	1	0	1	0	0	0	0	0	0	1	1	0	0	0	0
Luciogobius pallidus	NT	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
Luciogobius spp.		0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	1	0	0	1	1	0	0	0	0	0
Mugilogobius abei		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	1	0	1	0	1	0
Myersina filifer		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Odontamblyopus lacepedii	VU	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Periophthalmus modestus	NT	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
Pseudogobius masago	VU	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
Rhinogobius flumineus		0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	1	1	0	0	1	0	0	0	0
Rhinogobius similis		0	0	0	0	0	0	1	1	1	0	1	1	1	1	1	0	0	1	0	1	1	1	1	1	1	1
Rhinogobius sp.		1	1	1	1	0	1	1	1	1	1	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1	1
Sicyopterus japonicus		0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0
Taenioides snyderi	EN	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	1	1	1	1
Tridentiger barbatus	NT	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Tridentiger trigonocephalus		0	0	0	0	1	1	1	0	0	1	0	0	0	0	0	1	0	0	0	1	0	0	0	1	0	1
Tridentiger spp.		1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Parioglossus dotui		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1
Scatophagus argus		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1
Siganus fuscescens		0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0
Prionurus scalprum		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Sphyraena japonica		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Sphyraena pinguis		0	0	0	0	0	1	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1
Auxis spp.		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Sarda orientalis		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Scomber spp.		1	0	0	0	1	1	1	0	1	0	1	0	1	0	1	1	0	0	0	0	1	1	0	0	0	0
Scomberomorus niphonius		0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0
Channa argus	AL	0	1	0	0	0	0	0	0	1	0	1	0	1	0	1	0	0	0	0	0	1	1	0	0	0	0
Paralichthys olivaceus		0	0	1	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
Eopsetta grigorjewi		0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Platichthys stellatus		1	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Platichthys bicoloratus		0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	1	1	0	1	0	0	0	0
Thamnaconus modestus		0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Canthigaster rivulata		0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Takifugu spp.		1	1	1	1	1	1	1	1	1	1	0	1	0	1	1	1	1	1	1	1	1	1	1	1	1	1

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Kume, M., Lavergne, E. (2022). Fish Diversity of Estuaries Revealed by Environmental DNA. In: Kai, Y., Motomura, H., Matsuura, K. (eds) Fish Diversity of Japan. Springer, Singapore. https://doi.org/10.1007/978-981-16-7427-3_9

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DOI: https://doi.org/10.1007/978-981-16-7427-3_9
Published: 21 January 2022
Publisher Name: Springer, Singapore
Print ISBN: 978-981-16-7426-6
Online ISBN: 978-981-16-7427-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

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Fish Diversity of Estuaries Revealed by Environmental DNA

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