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Applications of Fish Cell Cultures

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Biotechnological Advances in Aquaculture Health Management

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Abstract

Cell culture serves as a reliable and proficient tool in diverse research fields such as virology, physiology, toxicology, immunology, oncology, genetics, and pharmacology. These systems can be employed for pathogen detection, confirmation, and characterization especially of viruses. It is also applicable in the case of intracellular bacteria, myxosporean or microsporean parasites as well. Fish cell cultures have gained more popularity in recent years and have prominent roles in viral disease diagnosis. Since treatment options are limited for many viral diseases, early disease diagnosis and proactive management measures are key for successful fish health management. The ability to propagate fish viruses in vitro using cell cultures is imperative in advancing research on viruses and to facilitate disease management strategies such as vaccines and antiviral agents. Moreover, potential host range of pathogens via susceptibility to cell cultures, virus-host cell interactions, and virus localization studies using cell cultures provide a better understanding of the viral pathogenesis. Availability of suitable fish cell cultures for propagation of viruses and disease diagnosis is very limited, which is a major concern in this area. The wide array of applications exemplifies the versatility, cost-effectiveness, and high potential of fish cell cultures in various research fields. The recent swift growth observed in research employing cell cultures is definitely an outcome of the progress in this sector and also due to increasing ethical demands for reduction and replacement of animal use in research. In the near future, innovations in 3D cell culture and CRISPER-Cas9 genome editing will further enhance the research prospects of fish cell culture systems.

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Acknowledgments

The authors acknowledge financial support from the Mississippi Agricultural and Forestry Experiment Station (MAFES) Strategic Research Initiative and the US Department of Agriculture—Agricultural Research Service—Catfish Health Initiative.

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Authors and Affiliations

  1. Thad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, USA

    Suja Aarattuthodi & Vandana Dharan

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  1. Suja Aarattuthodi
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  2. Vandana Dharan
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Correspondence to Suja Aarattuthodi .

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  1. School of Molecular Diagnostic Prophylactics and Nano-Biotechnology (SMDPN), ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India

    Sanjay Kumar Gupta

  2. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea (Republic of)

    Sib Sankar Giri

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Aarattuthodi, S., Dharan, V. (2021). Applications of Fish Cell Cultures. In: Gupta, S.K., Giri, S.S. (eds) Biotechnological Advances in Aquaculture Health Management . Springer, Singapore. https://doi.org/10.1007/978-981-16-5195-3_7

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