Abstract
Optimal fish health in recent times is clearly demarcated by sustainability in the upscaled aquacultural practices. Towards this, the application of selective genetic breeding techniques in farmed aquatic species generates tremendous potential for substantial production in terms of both the economically viable traits-growth and development. Acquiring such favourable changes in the undesired gene functions leads to greater genetic gain within organisms. So far, the methods of gene manipulation have focussed on the aspects of direct gene knockdown or gene elimination. However, recent advancements make use of efficient and targeted gene editing at random locus/loci within the genome. To date, a number of genes for a variety of traits have been employed for carrying out precise gene editing in different fish species. The major traits include those involved in reproduction, growth, development, pigmentation, disease resistance, etc. mainly among model fish species like zebrafish, medaka, etc. as well as economically relevant species such as Atlantic salmon, tilapia, rainbow trout, etc. Moreover, it has been significantly noted that among the different methods followed for incorporating the required genetic change, CRISPR Cas9 technique has successfully superseded the initial technologies in ensuring improved precision in editing at much broadened target sites, thus lowering the off-target rates while bringing about better gene activation or inhibition.
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Gupta, A., Gupta, R., Gupta, S.K. (2021). Gene Editing Technology for Fish Health Management. In: Gupta, S.K., Giri, S.S. (eds) Biotechnological Advances in Aquaculture Health Management . Springer, Singapore. https://doi.org/10.1007/978-981-16-5195-3_6
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