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Biotechnological Advances in Aquaculture Health Management pp 61–78Cite as

Application of Carbon Nanotubes in the Advancement of Fish Vaccine

Abstract

Various ways are adopted to control diseases in aquaculture. The development of efficient drug delivery system is of fundamental importance to improve effects of drug. In this regard, the unique properties of nanoparticles attracted extensive research on the applications of nanoparticles in aquaculture. Recently, nanodelivery system has been developed to improve the administration and efficacy of vaccines. Nanoparticles can be easily turned up to have specific chemical and physical characteristics. Likewise, carbon nanotubes (CNTs) are new alternative and efficient tool for transporting and translocating therapeutic molecules. As the functionalized CNTs are not immunogenic and have low toxicity, highly biocompatible, they hold tremendous potential in nanomedicine and nanobiotechnology. CNT-based drug delivery is promising for higher efficacy with lower side effects in achieving the higher effectiveness of drugs. CNTs are being utilized delivery vehicles for vaccines to protect farmed fish against disease-causing pathogens. This book chapter sheds the light on CNTs as a potential novel tool as vaccine carrier against various bacterial and viral diseases in fish. The importance of CNTs to enhance sustainable aquaculture has also been highlighted in this chapter.

Keywords

  • Nanoparticles
  • Carbon nanotubes
  • Fish
  • Pathogens
  • Diseases resistance

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References

  • Aklakur M, Asharf Rather M, Kumar N (2016) Nanodelivery: an emerging avenue for nutraceuticals and drug delivery. Crit Rev Food Sci Nutr 56:2352–2236

    CAS  PubMed  Google Scholar 

  • Alderman DJ (1988) Fisheries chemotherapy: a review. In: Muir JF, Roberts RJ (eds) Recent advances in aquaculture. Springer, Dordrecht. ISBN: 978-94-011-9743-4

    Google Scholar 

  • Andón FT, Kapralov AA, Yanamala N, Feng W, Baygan A, Chambers BJ et al (2013) Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase. Small 9:2721–2729

    PubMed  PubMed Central  Google Scholar 

  • Battigelli B, Ménard-Moyona C, Bianco A (2014) Carbon nanomaterials as new tools for immunotherapeutic applications. J Mater Chem B 2:6144–6156

    CAS  PubMed  Google Scholar 

  • Bhattacharya K, Mukherjee SP, Gallud A, Burkert SC, Bistarelli S, Bellucci S, Bottini M, Star A, Fadeel B (2016) Biological interactions of carbon-based nanomaterials: from coronation to degradation. Nanomedicine 12:333–351

    CAS  PubMed  Google Scholar 

  • Boutier M, Gao Y, Donohoe O, Vanderplasschen A (2019) Current knowledge and future prospects of vaccines against cyprinid herpesvirus 3 (CyHV-3). Fish Shellfish Immunol 93:531–541

    CAS  PubMed  Google Scholar 

  • Boyle D, Sutton PA, Handy RD, Henry TB (2018) Intravenous injection of unfunctionalized carbon-based nanomaterials confirms the minimal toxicity observed in aqueous and dietary exposures in juvenile rainbow trout (Oncorhynchus mykiss). Environ Pollut 232:191–199

    CAS  PubMed  Google Scholar 

  • Corbeil S, LaPatra SE, Anderson ED, Jones J, Vincent B, Hsu YL, Kurath G (1999) Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines. Dis Aquat Org 39:29–36

    CAS  Google Scholar 

  • Dhar AK, Manna SK, Allnutt FCT (2014) Viral vaccines for farmed finfish. Virus Dis 25(1):1–17

    Google Scholar 

  • Evelyn TPT (1997) A historical review of fish vaccinology. In: Gudding R, Lillehaug A, Midslyng PJ, Brown F (eds) Developments in biological standardization: fish vaccinology. International Association of Biological Standardization, vol 90. Karger, Basel, pp 3–12

    Google Scholar 

  • Fadel TR, Fahmy TM (2014) Immunotherapy applications of carbon nanotubes: from design to safe applications. Trends Biotechnol 32(4):198–209

    CAS  PubMed  Google Scholar 

  • FAO (2018) The state of world fisheries and aquaculture 2018—meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.

    Google Scholar 

  • Florence AT, Hillery AM, Hussain N, Jani PU (1995) Nanoparticles as carriers for oral peptide absorption: studies on particle uptake and fate. J Control Release 36:39–44

    CAS  Google Scholar 

  • Fraser TW, Reinardy HC, Shaw BJ, Henry TB, Handy RD (2011) Dietary toxicity of single-walled carbon nanotubes and fullerenes (C60) in rainbow trout (Oncorhynchus mykiss). Nanotoxicology 5(1):98–108

    CAS  PubMed  Google Scholar 

  • Giri SS, Kim SG, Kang JW, Kim SW, Kwon J, Lee SB, Jung WJ, Park SC (2021) Applications of carbon nanotubes and polymeric micro-/nanoparticles in fish vaccine delivery: progress and future perspectives. Rev Aquacult. https://doi.org/10.1111/raq.12547

  • Gomez-Casado E, Estepa A, Coll JM (2011) A comparative review on European-farmed finfish RNA viruses and their vaccines. Vaccine 29:2657–2671

    CAS  PubMed  Google Scholar 

  • Gong YX, Zhu B, Liu G-L, Liu L, Ling F, Wang G-X, Xu GX (2015) Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila. Fish Shellfish Immunol 42:213–220

    CAS  PubMed  Google Scholar 

  • Gregory AE, Titball R, Williamson D (2013) Vaccine delivery using nanoparticles. Front Cell Infect Microbiol 3:1–13

    Google Scholar 

  • Guo CJ, Wu YY, Yang LS, Yang XB, He J, Mi S, Jia KT, Weng SP, Yu XQ, He JG (2012) Infectious spleen and kidney necrosis virus (a fish iridovirus) enters Mandarin fish fry cells via caveola-dependent endocytosis. J Virol 86(5):2621–2631

    CAS  PubMed  PubMed Central  Google Scholar 

  • Guo Z, Lin Y, Wang X, Fu Y, Lin W, Lin X (2018) The protective efficacy of four iron-related recombinant proteins and their single-walled carbon nanotube encapsulated counterparts against Aeromonas hydrophila infection in zebrafish. Fish Shellfish Immunol 82:50–59

    CAS  PubMed  Google Scholar 

  • Guo ZR, Zhao Z, Zhang C, Jia YJ, Qiu DK, Zhu B, Wang GX (2020) Carbon nanotubes-loaded subunit vaccine can increase protective immunity against rhabdovirus infections of largemouth bass (Micropterus salmoides). Fish Shellfish Immunol 99:548–554

    CAS  PubMed  Google Scholar 

  • Hu F, Li Y, Wang Q, Wang G-X, Zhu B, Wang Y, Zeng W, Yin J, Liu C, Bergmann SM, Shi C (2020) Carbon nanotube-based DNA vaccine against koi herpesvirus given by intramuscular injection. Fish Shellfish Immunol 98:810–818

    CAS  PubMed  Google Scholar 

  • Hu F, Li Y, Wang Q, Zhu B, Wu S, Wang Y, Zeng W, Yin J, Liu C, Bergmann SM, Shi C (2021) Immersion immunization of koi (Cyprinus carpio) against cyprinid herpesvirus 3 (CyHV-3) with carbon nanotube-loaded DNA vaccine. Aquaculture 539:736644

    CAS  Google Scholar 

  • Huising MO, Guichelaar T, Hoek C, Verburg-Van Kemenade BML, Flik G, Savelkoul HFJ, Rombout J (2003) Increased efficacy of immersion vaccination in fish with biohyperosmotic pretreatment. Vaccine 21:4178–4193

    CAS  PubMed  Google Scholar 

  • Ji J, Torrealba D, Ruyra A, Roher N (2015) Nanodelivery systems as new tools for immunostimulant or vaccine administration: targeting the fish immune system. Biology 4:664–696

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jia YJ, Guo ZR, Ma R, Qiu DK, Zhao Z, Wang GX, Zhu B (2020a) Immune efficacy of carbon nanotubes recombinant subunit vaccine against largemouth bass ulcerative syndrome virus. Fish Shellfish Immunol 100:317–323

    CAS  PubMed  Google Scholar 

  • Jia Y-J, Guo Z-R, Ma R, Qiu D-K, Wang G-X, Zhu B (2020b) Protective immunity of largemouth bass immunized with immersed DNA vaccine against largemouth bass ulcerative syndrome virus. Fish Shellfish Immunol 107:269–276

    CAS  PubMed  Google Scholar 

  • Kim M-G, Park JY, Shon Y, Kim G, Shim G, Oh OY-K (2014) Nanotechnology and vaccine development. Asian J Pharm Sci 9(5):227–235

    Google Scholar 

  • Kyriakides TR, Raj A, Tseng TH, Xiao H, Nguyen R, Mohammed FS, Halder SS, Xu M, Wu MJ, Bao S, Sheu WC (2021) Biocompatibility of nanomaterials and their immunological properties. Biomed Mater. https://doi.org/10.1088/1748-605X/abe5fa

  • Lijuan Z, Ningqiu L, Qiang L, Lihui L (2018) An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection. Fish Shellfish Immunol 77:474–480

    PubMed  Google Scholar 

  • Liu L, Gong Y-X, Liu G-L, Zhu B, Wang G-X (2016) Protective immunity of grass carp immunized with DNA vaccine against Aeromonas hydrophila by using carbon nanotubes as a carrier molecule. Fish Shellfish Immunol 55:516–522

    CAS  PubMed  Google Scholar 

  • Liu G-Y, Wang E-L, Qu X-Y, Yang K-C et al (2020) Single-walled carbon nanotubes enhance the immune protective effect of a bath subunit vaccine for pearl gentian grouper against Iridovirus of Taiwan. Fish Shellfish Immunol 106:510–517

    CAS  PubMed  Google Scholar 

  • Liu G, Li J, Zhang Z, Liu J, Zhang C, Zhu B et al (2021) An immersion subunit vaccine loaded by single-walled carbon nanotube protects pearl gentian grouper from viral nervous necrosis virus. Aquaculture 541:736813

    CAS  Google Scholar 

  • Luis AIS, Ramos Campos EV, de Oliveira JL, Fraceto LF (2019) Trends in aquaculture sciences: from now to use of nanotechnology for disease control. Rev Aquac 11:119–132

    Google Scholar 

  • Mitchell H (1995) Choosing a furunculosis vaccine: points to consider. Bull Aquac Assoc Can 95:30–37

    Google Scholar 

  • Nakanishi T, Ototake M (1997) Antigen uptake and immune responses after immersion vaccination. Dev Biol Stand 90:59–68

    CAS  PubMed  Google Scholar 

  • Nakanishi T, Kiryu I, Ototake M (2002) Development of a new vaccine delivery method for fish: percutaneous administration by immersion with application of a multiple puncture instrument. Vaccine 20:3764–3769

    CAS  PubMed  Google Scholar 

  • Oyewumi MO, Kumar A, Cui Z (2010) Nano-microparticles as immune adjuvants: correlating particle sizes and the resultant immune responses. Expert Rev Vaccines 9:1095–1107

    CAS  PubMed  PubMed Central  Google Scholar 

  • Peek LJ, Middaugh CR, Berkland C (2008) Nanotechnology in vaccine delivery. Adv Drug Deliv Rev 60:915–928

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pescatori M, Bedognetti D, Venturelli E, Ménard-Moyon C, Bernardini C, Muresu E et al (2013) Functionalized carbon nanotubes as immunomodulator systems. Biomaterials 34:4395–4403

    CAS  PubMed  Google Scholar 

  • Plant KP, LaPatra SE (2011) Advances in fish vaccine delivery. Dev Comp Immunol 35:1256–1262

    CAS  PubMed  Google Scholar 

  • Qiu D-K, Zhao Z, Ma R, Guo Z-R, Jia Y-J, Zhang C, Wang G-X, Zhu B (2020) Antigen epitope screening of grass carp reovirus and its protectively immunity assessment for grass carp. Aquaculture 515:734550

    CAS  Google Scholar 

  • Qiu D-K, Jia Y-J, Gong Y-M, Zheng Y-Y, Wang G-X, Zhu B (2021) Optimizing the immunization procedure of single-walled carbon nanotubes based vaccine against grass carp reovirus for grass carp. Aquaculture 533:736152

    CAS  Google Scholar 

  • Ringø E, Olsen RE, Jensen I et al (2014) Application of vaccines and dietary supplements in aquaculture: possibilities and challenges. Rev Fish Biol Fish 24:1005–1032

    Google Scholar 

  • Scheinberg DA, McDevitt MR, Dao T, Mulvey JJ, Feinberg E, Alidori S (2013) Carbon nanotubes as vaccine scaffolds. Adv Drug Deliv Rev 65(15):2016–2022

    CAS  PubMed  Google Scholar 

  • Shah BR, Mraz J (2020) Advances in nanotechnology for sustainable aquaculture and fisheries. Rev Aquac 12:925–942. https://doi.org/10.1111/raq.12356

    CrossRef  Google Scholar 

  • Smith CJ, Shaw BJ, Handy RD (2007) Toxicity of single walled carbon nanotubes to rainbow trout, (Oncorhynchus mykiss): respiratory toxicity, organ pathologies, and other physiological effects. Aquat Toxicol 82:94–109

    CAS  PubMed  Google Scholar 

  • Sohn EK, Chung YS, Johari SA, Kim TG, Kim JK, Lee JH, Lee YH, Kang SW, Yu IJ (2015) Acute toxicity comparison of single-walled carbon nanotubes in various freshwater organisms. Biomed Res Int 2015:323090

    PubMed  PubMed Central  Google Scholar 

  • Song CK, Oh E, Kang MS, Shin BS, Han SY, Jung M, Lee ES, Yoon SY, Sung MM, Ng WB, Cho NJ, Lee H (2018) Fluorescence-based immunosensor using threedimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker. Anal Chim Acta 1027:101–108

    CAS  PubMed  Google Scholar 

  • Vinay TN, Bhat S, Gon Choudhury T, Paria A, Jung M-H, Shivani Kallappa G, Jung S (2018) Recent advances in application of nanoparticles in fish vaccine delivery. Rev Fish Sci Aquac 26:29–41

    Google Scholar 

  • Wang Y, Liu GL, Li DL, Ling F, Zhu B, Wang GX (2015) The protective immunity against grass carp reovirus in grass carp induced by a DNA vaccination using single-walled carbon nanotubes as delivery vehicles. Fish Shellfish Immunol 47(2):732–742

    CAS  PubMed  Google Scholar 

  • Zhang C, Zhao Z, Zha J-W, Wang G-X, Zhu B (2017) Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of a DNA vaccine against spring viremia of carp virus in common carp. Fish Shellfish Immunol 71:191–201

    CAS  PubMed  Google Scholar 

  • Zhang C, Zhao Z, Liu GY, Li J, Wang GX, Zhu B (2018) Immune response and protective effect against spring viremia of carp virus induced by intramuscular vaccination with a SWCNTs-DNA vaccine encoding matrix protein. Fish Shellfish Immunol 79:256–264

    CAS  PubMed  Google Scholar 

  • Zhang C, Zheng YY, Gong YM, Zhao Z, Guo ZR, Jia YJ, Wang GX, Zhu B (2019) Evaluation of immune response and protection against spring viremia of carp virus induced by a single-walled carbon nanotubes-based immersion DNA vaccine. Virology 537:216–225

    CAS  PubMed  Google Scholar 

  • Zhang C, Wang GX, Zhu B (2020a) Application of antigen presenting cell-targeted nanovaccine delivery system in rhabdovirus disease prophylactics using fish as a model organism. J Nanobiotechnol 18(1):24

    CAS  Google Scholar 

  • Zhang Z, Liu G, Ma R, Qi X, Wang G-X, Zhu B, Ling F (2020b) The immunoprotective effect of whole-cell lysed inactivated vaccine with SWCNT as a carrier against Aeromonas hydrophila infection in grass carp. Fish Shellfish Immunol 97:336–343

    CAS  PubMed  Google Scholar 

  • Zhao Z, Zhang C, Lin Q et al (2020a) Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of an immersion DNA vaccine against infectious spleen and kidney necrosis virus in mandarin fish. Fish Shellfish Immunol 97:432–439

    CAS  PubMed  Google Scholar 

  • Zhao Z, Xiong Y, Zhang C, Jia Y-J, Qiu D-K, Wang G-X, Zhu B (2020b) Optimization of the efficacy of a SWCNTs-based subunit vaccine against infectious spleen and kidney necrosis virus in mandarin fish. Fish Shellfish Immunol 106:190–196

    CAS  PubMed  Google Scholar 

  • Zhao Z, Ma R, Guo Z-R, Zhang C, Xiong Y, Wang G-X, Zhu B (2021) Single-walled carbon nanotubes as drug carrier loaded with moroxydine hydrochloride against infectious spleen and kidney necrosis virus in mandarin fish. Aquaculture 536:736469

    CAS  Google Scholar 

  • Zheng Y-Y, Qiu D-K, Guo Z-R, Gong Y-M, Wang G-X, Zhu B (2021) Evaluation of SWCNTs loaded DNA vaccine encoding predominant antigen epitope VP4-3 against type II GCRV. Aquaculture 534:736197

    CAS  Google Scholar 

  • Zhu B, Liu GL, Gong YX, Ling F, Song LS, Wang GX (2014) Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus. Fish Shellfish Immunol 41(2):279–293

    CAS  PubMed  Google Scholar 

  • Zhu B, Liu GL, Gong YX, Ling F, Wang GX (2015) Protective immunity of grass carp immunized with DNA vaccine encoding the vp7 gene of grass carp reovirus using carbon nanotubes as a carrier molecule. Fish Shellfish Immunol 42:325–334

    CAS  PubMed  Google Scholar 

  • Zhu B, Zhang C, Zhao Z, Wang G-X (2020) Targeted delivery of mannosylated nanoparticles improve prophylactic efficacy of immersion vaccine against fish viral disease. Vaccine 8(1):87

    CAS  Google Scholar 

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Acknowledgment

Authors acknowledge the “Brain Pool program” of the National Research Foundation of Korea, Ministry of Science and ICT (KRF: 2016H1D3A1909005).

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Authors and Affiliations

  1. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea

    Sib Sankar Giri & Se Chang Park

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  1. Sib Sankar Giri
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  2. Se Chang Park
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Correspondence to Se Chang Park .

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Editors and Affiliations

  1. School of Molecular Diagnostic Prophylactics and Nano-Biotechnology (SMDPN), ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India

    Dr. Sanjay Kumar Gupta

  2. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea (Republic of)

    Dr. Sib Sankar Giri

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Giri, S.S., Park, S.C. (2021). Application of Carbon Nanotubes in the Advancement of Fish Vaccine. In: Gupta, S.K., Giri, S.S. (eds) Biotechnological Advances in Aquaculture Health Management . Springer, Singapore. https://doi.org/10.1007/978-981-16-5195-3_4

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