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Experimental Methods for Determination of Protein–Protein Interactions

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Protein-Protein Interactions

Abstract

Study of protein–protein interactions in the cell provides better insights into the molecular network of the cell. It is therefore essential to establish the existing interactions by experimental techniques. Such techniques not only help to discover new interactions, but also validate interactions that have been hypothesized. The techniques can be broadly classified as biophysical, biochemical, and genetic. This chapter discusses the techniques under each classification and also outlines their respective working principles, advantages, drawbacks, and recent developments. The future prospects have been delineated based on their current status of applications.

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    Krishna Mohan Poluri, Khushboo Gulati & Sharanya Sarkar

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Poluri, K.M., Gulati, K., Sarkar, S. (2021). Experimental Methods for Determination of Protein–Protein Interactions. In: Protein-Protein Interactions. Springer, Singapore. https://doi.org/10.1007/978-981-16-1594-8_5

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