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Detection of the Avidin–Biotin Reaction

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Acoustic Levitation-Based Trace-Level Biosensing

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Abstract

In this section, a detection scheme based on the levitation coordinate shift of a microparticle by the binding of AuNPs is demonstrated. The relationship between the particle density and the levitation coordinate is studied using gold-plated PMMA microparticles in Sect. 4.2. In addition, the levitation coordinate change of epoxy microparticles (EPs) by AuNP-binding is discussed in Sect. 4.3. A zmol sensing scheme based on the levitation of a single PS microparticle is shown in Sect. 4.4. Finally, this method was applied to the quantification of dissolved biotin in Sect. 4.5.

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Correspondence to Akihisa Miyagawa .

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Miyagawa, A. (2021). Detection of the Avidin–Biotin Reaction. In: Acoustic Levitation-Based Trace-Level Biosensing. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-16-1425-5_4

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