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Initiation and Establishment of Cell Suspension Cultures in Bamboo

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Abstract

Bamboos have peculiar reproductive characteristics, such as irregular flowering, low seed viability, and monocarpic nature, which make it difficult to introduce characteristics of interest by classical breeding. In addition, conventional breeding methods are considered inefficient when aiming at mass production. In this sense, biotechnological tools, such as protoplast culture, somatic hybridization, somatic embryogenesis, and genetic transformation, in addition to vegetative propagation, are configured as important alternative methods to subsidize programs of genetic improvement of bamboo and boost mass-scale production of interest varieties and superior genotypes. However, for the application of the aforementioned techniques, the establishment and the ability to control the multiplication of cell suspensions, with a high growth rate and with interest characteristics, seem to be of fundamental importance. Although the direction of research for the establishment of cell suspensions in bamboo has been reported since the 1980s, currently there are relatively few advances obtained, with limited work on the number of species used. In this sense, this chapter compiles the available works on the establishment of cell suspensions in bamboo, with emphasis on the two species of Guadua, G. magna, and G. aff. chaparensis, and describes a pioneering and detailed methodology of easy application for the establishment and maintenance of cell suspensions with embryogenic potential.

Keywords

  • Callus culture
  • Cell suspension culture
  • Micropropagation
  • Secondary metabolites
  • Somatic embryogenesis

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Acknowledgments

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Grant 458151/2013-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes/Embrapa 001-2011/Grant 39), and Centro de Pesquisa e Aplicação de Bambu e Fibras Naturais (CPAB) for their financial support and fellowships.

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The authors declare that they have no conflict of interest.

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Correspondence to Jonny Everson Scherwinski-Pereira .

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© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

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Queiroz, F.F., de Araújo Silva-Cardoso, I.M., Scherwinski-Pereira, J.E. (2021). Initiation and Establishment of Cell Suspension Cultures in Bamboo. In: Ahmad, Z., Ding, Y., Shahzad, A. (eds) Biotechnological Advances in Bamboo. Springer, Singapore. https://doi.org/10.1007/978-981-16-1310-4_5

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