Abstract
Optogenetics brought noninvasive neural activation in living organisms. Transparent zebrafish larva is one of the suitable animal models that receive the full benefit of this technique and provides behavioral studies based on intact individual nervous system. In this chapter, we describe methods to introduce optogenetic genes into zebrafish, and desirable apparatus for photostimulation and motion analysis with an example from our studies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- CiD neuron:
-
Circumferential ipsilateral descending neuron
- CoPA neuron:
-
Commissural primary ascending neuron
- DLF:
-
Dorsal longitudinal fascicule
- dpf:
-
Days postfertilization
- IC neuron:
-
Ipsilateral projecting neuron
- PTU:
-
Phenylthiourea
- RB neuron:
-
Rohon-Beard neuron
- RS neuron:
-
Reticulospinal neuron
References
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003) Translocation of CaM kinase II to synaptic sites in vivo. Nat Neurosci 6:217–218
Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol 31:227–229
Kawakami K (2007) Tol2: a versatile gene transfer vector in vertebrates. Genome Biol 8(Suppl 1):S7
Kimura Y, Hisano Y, Kawahara A, Higashijima S (2014) Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering. Sci Rep 4:6545
Li M, Zhao L, Page-McCaw PS, Chen W (2016) Zebrafish genome engineering using the CRISPR-Cas9 system. Trends Genet 32:815–827
Pietri T, Manalo E, Ryan J, Saint-Amant L, Washbourne P (2009) Glutamate drives the touch response through a rostral loop in the spinal cord of zebrafish embryos. Dev Neurobiol 69:780–795
Umeda K, Ishizuka T, Yawo H, Shoji W (2016) Position- and quantity-dependent responses in zebrafish turning behavior. Sci Rep 6:27888
Villefranc JA, Amigo J, Lawson ND (2007) Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn 236:3077–3087
White RM, Sessa A, Burke C, Bowman T, LeBlanc J, Ceol C, Bourque C, Dovey M, Goessling W, Burns CE, Zon LI (2008) Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell 2:183–189
Acknowledgments
The studies described here were supported by FRIS promoted research grant. I thank to the National Bioresource Project (NBRP), Japan for supplying a transgenic zebrafish strain in the work.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Shoji, W. (2021). Optogenetics on Zebrafish. In: Yawo, H., Kandori, H., Koizumi, A., Kageyama, R. (eds) Optogenetics. Advances in Experimental Medicine and Biology, vol 1293. Springer, Singapore. https://doi.org/10.1007/978-981-15-8763-4_21
Download citation
DOI: https://doi.org/10.1007/978-981-15-8763-4_21
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-15-8762-7
Online ISBN: 978-981-15-8763-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)