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Strategies and Advancement in Growth Factor Immobilizable ECM for Tissue Engineering

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Immobilization Strategies

Part of the book series: Gels Horizons: From Science to Smart Materials ((GHFSSM))

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Abstract

Immobilization of functional molecules in biotechnology is extremely important. Growth factors are biomolecules with high bioactivity on cells and are very useful for culture engineering and regenerative medicine applications. However, their poor stability and high cost are challenges for practical application. Immobilization of growth factors has been conducted by various methods to localize growth factors and to keep high local concentrations. In this chapter, previous methods to immobilize growth factors are outlined. Thereafter, organ-specific extracellular matrix (ECM) and ECM-modelized material as growth factor immobilizable materials are mentioned with focus on their properties and their applications such as regenerative medicine.

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Abbreviations

ECM:

Extracellular matrix

NGF:

Nerve growth factor

HGF:

Hepatocyte growth factor

EGF:

Epidermal growth factor

bFGF:

Basic fibroblast growth factor

VEGF:

Vascular endothelial growth factor

GAG:

Glycosaminoglycan

L-ECM:

Liver-specific extracellular matrix

TX100:

Triton X-100

PBS:

Phosphate buffered saline

H&E staining:

Hematoxylin & Eosin staining

DNA:

Deoxyribonucleic acid

ELISA:

Enzyme-linked immuno-sorbent assay

PUF:

Polyurethane foam

Hep-col:

Heparin-conjugated collagen

Hep-gela:

Heparin-conjugated gelatin

NHS:

N-hydroxysuccinimide

MES:

2-morpholinoethanesulfonic acid, monohydrate

EDC:

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide

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Authors and Affiliations

  1. Department of Chemical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Y. Ikegami & H. Ijima

Authors
  1. Y. Ikegami
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  2. H. Ijima
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Correspondence to H. Ijima .

Editor information

Editors and Affiliations

  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India

    Anuj Tripathi

  2. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India

    Jose Savio Melo

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Ikegami, Y., Ijima, H. (2021). Strategies and Advancement in Growth Factor Immobilizable ECM for Tissue Engineering. In: Tripathi, A., Melo, J.S. (eds) Immobilization Strategies . Gels Horizons: From Science to Smart Materials. Springer, Singapore. https://doi.org/10.1007/978-981-15-7998-1_3

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