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Microbial Biosurfactants and Their Potential Applications: An Overview

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Microbial Biosurfactants

Part of the book series: Environmental and Microbial Biotechnology ((EMB))

Abstract

Biosurfactants are amphiphilic and one of the most versatile compounds synthesized by certain plants and microorganisms. These compounds are well known for their wide range of applications from domestic, personal, food and medical up to industrial level. They are capable of emulsifying oily substances which makes them very useful as a cleaning agent for domestic or industrial purpose and at the same time makes it competent for the exploration of oil through microbial enhanced oil recovery (MEOR) process. Besides they have a major application in the form of a potential tool to fight against petroleum-based contamination of oil and water. They are also used as an anti-adhesive agent, emulsifying agent in bakery industry, drug delivery system, etc. They are synthesized by a number of microorganisms such as Pseudomonas sp., Bacillus sp., Acinetobacter sp., Candida sp., Sphingomonas sp., Cryptococcus sp., Pseudozyma sp., Kurtzmanomyces sp., Rhodococcus sp., Arthrobacter sp., Lactococcus sp., Penicillium sp., Aspergillus sp., etc. and are encoded by a number of genes. In this regard, this chapter focus on basic chemical properties of various types of biosurfactants, source of production, name of genes responsible for its production, and various applications.

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References

  • Adebusoye SA, Amund OO, Ilori MO, Domeih DO, Okpuzor J (2008) Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria. Int J Quot Trop Biol 56:1603–1611

    Google Scholar 

  • Aparna A, Srinikethan G, Smitha H (2012) Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf B Biointerfaces 95:23–29

    Article  CAS  PubMed  Google Scholar 

  • Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R (2010) Microorganism in environmental management: microbes and environment. Appl Microbiol Biotechnol 87:427–444

    Article  CAS  PubMed  Google Scholar 

  • Beal R, Betts WB (2000) Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa. J Appl Microbiol 89(1):158–168

    Article  CAS  PubMed  Google Scholar 

  • Befkadu AA, Quanyuan CHEN (2018) Surfactant-enhanced soil washing for removal of petroleum hydrocarbons from contaminated soils: a review. Pedosphere 28(3):383–410

    Article  Google Scholar 

  • Behera BK, Prasad R (2020) Environmental technology and sustainability. Elsevier (ISBN: 9780128191033). https://www.elsevier.com/books/environmental-technology-and-sustainability/behera/978-0-12-819103-3

  • Bordoloi NK, Konwar BK (2008) Microbial surfactant-enhanced mineral oil recovery under laboratory conditions. Colloids Surf B Biointerfaces 63(1):73–82

    Article  CAS  PubMed  Google Scholar 

  • Bouassida M, Fourati N, Ghazala I, Ellouze-Chaabouni S, Ghribi D (2018) Potential application of Bacillus subtilis SPB1 biosurfactants in laundry detergent formulations: compatibility study with detergent ingredients and washing performance. Eng Life Sci 18(1):70–77

    Article  CAS  PubMed  Google Scholar 

  • Cameotra SS, Makkar RS (2010) Biosurfactant-enhanced bioremediation of hydrophobic pollutants. Pure Appl Chem 82(1):97–116

    Article  CAS  Google Scholar 

  • Cerniglia CE (1992) Biodegradation of polycyclic aromatic hydrocarbons. Int Biodegrad 3:351–368

    Article  CAS  Google Scholar 

  • Chan XY, Chang CY, Hong KW, Tee KK, Yin WF, Chan KG (2013) Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis. Gut Pathog 5(1):29

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chaprão MJ, Ferreira IN, Correa PF, Rufino RD, Luna JM, Silva EJ, Sarubbo LA (2015) Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand. Electron J Biotechnol 18(6):471–479

    Article  Google Scholar 

  • Das P, Mukherjee S, Sen R (2008) Genetic regulations of the biosynthesis of microbial surfactants: an overview. Biotechnol Genet Eng 25(1):165–186

    Article  CAS  Google Scholar 

  • De S, Malik S, Ghosh A, Saha R, Saha B (2015) A review on natural surfactants. RSC Adv 5(81):65757

    Article  CAS  Google Scholar 

  • Eswari JS, Dhagat S, Mishra P (2018) Biosurfactant assisted silver nanoparticle synthesis: a critical analysis of its drug design aspects. Adv Nat Sci Nanosci 9(4):045007

    Article  CAS  Google Scholar 

  • Fakruddin MD (2012) Biosurfactant: production and application. J Pet Environ Biotechnol 3(4):124

    Google Scholar 

  • Farias CB, Ferreira Silva A, Diniz Rufino R, Moura Luna J, Gomes Souza JE, Sarubbo LA (2014) Synthesis of silver nanoparticles using a biosurfactant produced in low-cost medium as stabilizing agent. Electron J Biotechnol 17(3):122–125

    Article  CAS  Google Scholar 

  • Felix AKN, Martins JJ, Almeida JGL, Giro MEA, Cavalcante KF, Melo VMM, Pessoa ODL, Rocha MVP, Gonçalves LRB, de Santiago Aguiar RS (2019) Purification and characterization of a biosurfactant produced by Bacillus subtilis in cashew apple juice and its application in the remediation of oil-contaminated soil. Colloids Surf B Biointerfaces 175:256–263

    Article  CAS  Google Scholar 

  • Fooladi T, Moazami N, Abdeshahian P, Kadier A, Ghojavand H, Yusoff WMW, Hamid AA (2016) Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field. J Petrol Sci Eng 145:510–519

    Article  CAS  Google Scholar 

  • Ghosh S, Ghosh A, Riyajuddin S, Sarkar S, Chowdhury AH, Ghosh K, Islam SM (2015) Silver nanoparticles architectured HMP as a recyclable catalyst for tetramic acid and propiolic acid synthesis through CO2 capture at atmospheric pressure. Chem Cat Chem 12(4):1055–1067

    Google Scholar 

  • Gudina EJ, Rocha V, Teixeira JA, Rodrigues LR (2010) Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei sp. paracasei A20. Lett Appl Microbiol 50:419–424

    Article  CAS  PubMed  Google Scholar 

  • Gudiña EJ, Rangarajan V, Sen R, Rodrigues LR (2013) Potential therapeutic applications of biosurfactants. Trends Pharmacol Sci 34(12):667–675

    Article  PubMed  CAS  Google Scholar 

  • Hazra C, Kundu D, Chaudhari A, Jana T (2013) Biogenic synthesis, characterization, toxicity and photocatalysis of zinc sulfide nanoparticles using rhamnolipids from Pseudomonas aeruginosa BS01 as capping and stabilizing agent. J Chem Technol Biotechnol 88(6):1039–1048

    Article  CAS  Google Scholar 

  • Heinemann C, van Hylckama Vlieg JE, Janssen DB, Busscher HJ, van der Mei HC, Reid G (2000) Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131. FEMS Microbiol Lett 190(1):177–180

    Article  CAS  PubMed  Google Scholar 

  • Hewald S, Josephs K, Bölker M (2005) Genetic analysis of biosurfactant production in Ustilago maydis. Appl Environ Microbiol 71(6):3033–3040

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hommel RK (1990) Formation and physiological role of biosurfactants produced by hydrocarbon utilizing microorganisms. Biodegradation 1:107–119

    Article  CAS  PubMed  Google Scholar 

  • https://www.sigmaaldrich.com/technical-documents/articles/biology/glycobiology/lipopolysaccharides.html. Accessed 16 Feb 2020

    Google Scholar 

  • Ibrahim ML, Ijah UJJ, Manga SB, Bilbis LS, Umar S (2013) Production and partial characterization of biosurfactant produced by crude oil degrading bacteria. Int Biodeter Biodegr 81:28–34

    Article  CAS  Google Scholar 

  • Jimoh AA, Lin J (2019) Biosurfactant: a new frontier for greener technology and environmental sustainability. Ecotox Environ Safe 184:109607

    Article  CAS  Google Scholar 

  • Joshi-Navare K, Khanvilkar P, Prabhune A (2013) Jatropha oil derived sophorolipids: production and characterization as laundry detergent additive. Biochem Res Int 2013:1–11

    Article  Google Scholar 

  • Khaje Bafghi M, Fazaelipoor MH (2012) Application of rhamnolipid in the formulation of a detergent. J Surfactant Deterg 15(6):679–684

    Article  CAS  Google Scholar 

  • Khan AHA, Tanveer S, Alia S, Anees M, Sultan A, Iqbal M, Yousaf S (2017) Role of nutrients in bacterial biosurfactant production and effect of biosurfactant production on petroleum hydrocarbon biodegradation. Ecol Eng 104:158–164

    Article  Google Scholar 

  • Khire JM (2010) Bacterial biosurfactants and their role in microbial enhanced oil recovery (MEOR). Adv Exp Med Biol 672:146–157

    Article  CAS  PubMed  Google Scholar 

  • Kulkarni P, Chakraborty R, Chakraborty S (2019) Biosurfactant mediated synthesis of silver nanoparticles using Lactobacillus brevis (MTCC 4463) and their antimicrobial studies. Int J Pharma Sci Res 10(4):1753–1759

    CAS  Google Scholar 

  • Liwarska-Bizukojc E, Galamon M, Bernat P (2018) Kinetics of biological removal of the selected micropollutants and their effect on activated sludge biomass. Water Air Soil Pollut 229(11):356

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Luna J, Rufino R, Campos G, Sarubbo L (2012) Properties of the biosurfactant produced by Candida sphaerica cultivated in low-cost substrates. Chem Eng 27:67–72

    Google Scholar 

  • Mireles JR, Toguchi A, Harshey RM (2001) Salmonella enterica serovar typhimurium swarming mutants with altered biofilm-forming abilities: surfactin inhibits biofilm formation. J Bacteriol 183(20):5848–5854

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mulligan CN (2005) Environmental applications for biosurfactants. Environ Pollut 133(2):183–198

    Article  CAS  PubMed  Google Scholar 

  • Pacwa-PÅ‚ociniczak M, PÅ‚aza GA, Piotrowska-Seget Z, Cameotra SS (2011) Environmental applications of biosurfactants: recent advances. Int J Mol Sci 12(1):633–654

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • PÅ‚aza GA, Chojniak J, Banat IM (2014) Biosurfactant mediated biosynthesis of selected metallic nanoparticles. Int J Mol Sci 15(8):13720–13737

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Prasad R (2014) Synthesis of silver nanoparticles in photosynthetic plants. J Nanoparticles. Article ID 963961. https://doi.org/10.1155/2014/963961

  • Prasad R, Jha A, Prasad K (2018) Exploring the realms of nature for nanosynthesis. Springer International Publishing (ISBN: 978-3-319-99570-0). https://www.springer.com/978-3-319-99570-0

  • Rane AN, Baikar VV, Kumar VR, Deopurkar RL (2017) Corrigendum: agro-industrial wastes for production of biosurfactant by Bacillus subtilis ANR 88 and its application in synthesis of silver and gold nanoparticles. Front Microbiol 8:878

    PubMed  PubMed Central  Google Scholar 

  • Reddy AS, Chen CY, Chen CC, Jean JS, Fan CW, Chen HR, Wang JC, Nimje VR (2009) Synthesis of gold nanoparticles via an environmentally benign route using a biosurfactant. J Nanosci Nanotechnol 9(11):6693–6699

    Article  CAS  PubMed  Google Scholar 

  • Rodrigues LR, Teixeira JA, Oliveira R (2006) Low-cost fermentative medium for biosurfactant production by probiotic bacteria. Biochem Eng J 32:135–142

    Article  CAS  Google Scholar 

  • Ron EZ, Rosenberg E (2002) Biosurfactants and oil bioremediation. Curr Opin Biotechnol 13(3):249–252

    Article  CAS  PubMed  Google Scholar 

  • Salusjärvi T, Povelainen M, Hvorslev N, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Neustroev KN, Kalkkinen N, Miasnikov AN (2004) Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia coli strain. Appl Microbiol Biotechnol 65(3):306–314

    Article  PubMed  CAS  Google Scholar 

  • Saravanakumari P, Mani K (2010) Structural characterization of a novel xylolipid biosurfactant from Lactococcus lactis and analysis of antibacterial activity against multi-drug resistant pathogens. Bioresour Technol 101(22):8851–8854

    Article  CAS  PubMed  Google Scholar 

  • Sharma D (2016a) Applications of biosurfactants in food. In: Biosurfactants in food. Springer, Cham, pp 43–80

    Chapter  Google Scholar 

  • Sharma D (2016b) Classification and properties of biosurfactants. In: Biosurfactants in food. Springer, Cham, pp 21–42

    Chapter  Google Scholar 

  • Sharma D, Saharan BS, Kapil S (2016) Biosurfactants of lactic acid bacteria. Springer, Cham

    Book  Google Scholar 

  • Shekhar S, Sundaramanickam A, Balasubramanian T (2015) Biosurfactant producing microbes and their potential applications: a review. Crit Rev Environ Sci Technol 45(14):1522–1554

    Article  CAS  Google Scholar 

  • Singh BR, Dwivedi S, Al-Khedhairy AA, Musarrat J (2011) Synthesis of stable cadmium sulfide nanoparticles using surfactin produced by Bacillus amyloliquefaciens strain KSU-109. Colloids Surf B Biointerfaces 85(2):207–213

    Article  CAS  PubMed  Google Scholar 

  • Singh BR, Singh BN, Khan W, Singh HB, Naqvi AH (2012) ROS-mediated apoptotic cell death in prostate cancer LNCaP cells induced by biosurfactant stabilized CdS quantum dots. Biomaterials 33(23):5753–5767

    Article  CAS  PubMed  Google Scholar 

  • Srivastava S, Usmani Z, Atanasov AG, Singh VK, Singh NP, Abdel-Azeem AM, Prasad R, Gupta G, Sharma M, Bhargava A (2021) Biological nanofactories: using living forms for metal nanoparticle synthesis. Mini-Rev Med Chem 21:245–265. https://doi.org/10.2174/1389557520999201116163012

    Article  PubMed  Google Scholar 

  • Stromberg LR, Heather MM, Harshini M (2017) Detection methods for lipopolysaccharides: past and present. In: Escherichia coli—recent advances on physiology, pathogenesis and biotechnological applications, pp 141–168

    Google Scholar 

  • Sullivan ER (1998) Molecular genetics of biosurfactant production. Curr Opin Biotechnol 9(3):263–269

    Article  CAS  PubMed  Google Scholar 

  • Tomar RS, Banerjee S, Kaushik S, Singh S, Vidyarthi AS (2015) Microbial synthesis of gold nanoparticles by biosurfactant producing Pseudomonas aeruginosa. Int J Adv Lif Sci 8(4):520–527

    CAS  Google Scholar 

  • Tugrul T, Cansunar E (2005) Detecting surfactant-producing microorganisms by the drop-collapse test. World J Microbiol Biotechnol 21(6–7):851–853

    Article  CAS  Google Scholar 

  • Van Bogaert IN, Holvoet K, Roelants SL, Li B, Lin YC, Van de Peer Y, Soetaert W (2013) The biosynthetic gene cluster for sophorolipids: a biotechnological interesting biosurfactant produced by Starmerella bombicola. Mol Microbiol 88(3):501–509

    Article  PubMed  CAS  Google Scholar 

  • Vaz DA, Gudina EJ, Alameda EJ, Teixeira JA, Rodrigues LR (2012) Performance of a biosurfactant produced by a Bacillus subtilis strain isolated from crude oil samples as compared to commercial chemical surfactants. Colloids Surf B Biointerfaces 89:167–174

    Article  CAS  PubMed  Google Scholar 

  • Vecino X, Cruz JM, Moldes AB, Rodrigues LR (2017) Biosurfactants in cosmetic formulations: trends and challenges. Crit Rev Biotechnol 37(7):911–923

    Article  CAS  PubMed  Google Scholar 

  • Velraeds MM, van der Mei HC, Reid G, Busscher HJ (1996) Physicochemical and biochemical characterization of biosurfactants released by Lactobacillus strains. Colloids Surf B Biointerfaces 8(1–2):51–61

    Article  CAS  Google Scholar 

  • Vijayakumar S, Saravanan V (2015) Biosurfactants-types, sources and applications. Res J Microbiol 10:181–192

    Article  Google Scholar 

  • Wang W, Cai B, Shao Z (2014) Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3. Front Microbiol 5:711

    Article  PubMed  PubMed Central  Google Scholar 

  • Wei YH, Lai CC, Chang JS (2007) Using Taguchi experimental design methods to optimize trace element composition for enhanced surfactin production by Bacillus subtilis ATCC 21332. Process Biochem 42(1):40–45

    Article  CAS  Google Scholar 

  • Yakimov MM, Timmis KN, Wray V, Freddrickson HL (1995) Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis BAS50. Appl Environ Microbiol 61:1706–1713

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McInerney MJ (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 56: 339 – 347

    Google Scholar 

  • Youssef N, Simpson DR, Duncan KE, McInerney MJ, Folmsbee M, Fincher T, Knapp RM (2007) In situ biosurfactant production by Bacillus strains injected into a limestone petroleum reservoir. Appl Environ Microbiol 73(4):1239–1247

    Article  CAS  PubMed  Google Scholar 

  • Zhou W, Wang X, Chen C, Zhu L (2013) Enhanced soil washing of phenanthrene by a plant-derived natural biosurfactant, Sapindus saponin. Colloids Surf A Physicochem Eng Asp 425:122–128

    Article  CAS  Google Scholar 

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Borah, D., Chaubey, A., Sonowal, A., Gogoi, B., Kumar, R. (2021). Microbial Biosurfactants and Their Potential Applications: An Overview. In: Inamuddin, Ahamed, M.I., Prasad, R. (eds) Microbial Biosurfactants. Environmental and Microbial Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-15-6607-3_5

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