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The Interplay Between Oviduct-Derived Exosomes and Cumulus-Oocyte Complexes

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Role of Exosomes in Biological Communication Systems

Abstract

As the mammalian oviduct is the place where the fertilization and preimplantation embryo development occur, the oviduct epithelial cells create a favorable environment for gamete/embryo development by secreting various growth factors. Recent research has focused on the important role of oviduct and its derivative for improving the fertilization as well as preimplantation embryo development. In particular, oviduct-derived exosomes have emerged as potential biomarkers for mediating cell-to-cell communication and transporting various genetic materials to recipient cells. According to many recent studies in reproduction field, the role of oviduct-derived exosomes on sperm and early embryo development is well established. However, there is still a lack of information on the effect and function of oviduct-derived exosomes on cumulus-oocyte complexes (COCs). Especially, the oviduct plays an important role for oocyte maturation process in canine species compared with other mammals, because when immature oocytes are ovulated from follicles, canine oocytes undergo maturation processes within the oviduct, while the other mammals already possess matured oocytes in preovulatory follicles. Also, none of the studies have investigated the relationship between oviduct-derived exosomes with COCs, except canine species. Therefore, this chapter focuses on the interaction between oviduct-derived exosomes and cumulus-oocyte complexes in dogs.

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Abbreviations

AREG:

Amphiregulin

COCs:

Cumulus-oocyte complexes

DNA:

Deoxyribonucleic acid

EGF:

Epidermal growth factor

EGFR:

Epidermal growth factor receptor

EREG:

Epiregulin

EVs:

Extracellular vesicles

FBS:

Fetal bovine serum

IVM:

In vitro maturation

MAPK:

Mitogen-activated protein kinase

miRNA:

Micro messenger ribonucleic acid

mRNA:

Messenger ribonucleic acid

NTA:

Nanoparticle tracking analysis

PBS:

Phosphate buffer saline

TEM:

Transmission electron microscopy

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Acknowledgments

The authors would like to acknowledge the Research Institute for Veterinary Science and the BK21 plus program and global PhD Fellowship Program through NRF who provided insight and expertise that greatly assisted the research.

Funding: This research was supported by the global PH.D Fellowship Program through NRF funded by the Ministry of Education (NRF-20142A1021187).

Disclosure of interests: All authors declare they have no conflict of interest.

Ethical approval for studies involving humans: This article does not contain any studies with human participants performed by any of the authors.

Ethical approval for studies involving animals: Animal experiments were performed following a standard procedure established by the Committee for Accreditation of Laboratory Animal Care and the Guideline for the Care and Use of Laboratory Animals of Seoul National University (approval number; SNU-140704-1).

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Authors and Affiliations

  1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea

    Seok Hee Lee

  2. Center for Reproductive Sciences, Department of Obstetrics and Gynecology, University of California San Francisco, San Francisco, CA, USA

    Seok Hee Lee

  3. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea

    Byeong Chun Lee

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Correspondence to Byeong Chun Lee .

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Editors and Affiliations

  1. Department of Biochemistry, ESC Research Unit, Faculty of Science King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

    Faisal A. Alzahrani

  2. Department of Animal Production, King Saud University, Riyadh, Saudi Arabia

    Islam M. Saadeldin

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Lee, S.H., Lee, B.C. (2021). The Interplay Between Oviduct-Derived Exosomes and Cumulus-Oocyte Complexes. In: Alzahrani, F.A., Saadeldin, I.M. (eds) Role of Exosomes in Biological Communication Systems. Springer, Singapore. https://doi.org/10.1007/978-981-15-6599-1_4

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