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Hyphenation

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Two-Dimensional Liquid Chromatography

Part of the book series: SpringerBriefs in Molecular Science ((BRIEFSMOLECULAR))

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Abstract

The identification of unknown compounds is of fundamental importance for a range of applications in chromatography including, but not limited to, environmental pollution, food/natural product analysis, metabolomics, sports drug testing and petrochemicals, and biofuel analysis. Critical to the success of each such application is the ability to separate the compounds of interest both from each other and the sample matrix-which may be present at concentrations many orders of magnitude higher than the target compounds. While two-dimensional chromatography increases the available separation space there can still be problems identifying all the peaks in a sample especially due to dilution of low concentration components in the second dimension. Selectivity and sensitivity are key to solving such problems. Both may be increased by the use of multiple dimensions of separation. In this chapter, some of the methods of extending the power of 2DLC by hyphenating it with other dimensions of separation are presented and discussed.

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Authors and Affiliations

  1. Australian Center for Research on Separation Science, School of Science, RMIT University, Bundoora West Campus, PO Box 71, Bundoora, VIC, 3083, Australia

    Oliver Jones

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  1. Oliver Jones
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Correspondence to Oliver Jones .

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Jones, O. (2020). Hyphenation. In: Two-Dimensional Liquid Chromatography. SpringerBriefs in Molecular Science. Springer, Singapore. https://doi.org/10.1007/978-981-15-6190-0_5

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