Abstract
Marine phytoplankton utilize the energy absorbed from natural sunlight through Photosystem II (PS II) complexes, to split water and convert the light energy into chemical energy; while excessive absorption of solar energy by the cells will result in the inactivation of PS II. Photoinactivation of PS II is an important component of the metabolic and eco-physiological processes in oxygen-evolving photoautotrophs; it is thus key to quantify the rate of PSII photoinactivation. This section describes in detail the methods and procedures for determination of PS II photoinactivation in phytoplankton, and particularly a method for estimating the rate constant of the damage to photosynthetic apparatus (k, in min−1) and the corresponding repair rate constant (r, in min−1) under inhibitory solar ultraviolet radiation (UVR), as well as matters needing attention during the operations.
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Li, G., Li, Y., Guan, W., Wu, H. (2021). Measurements of Photoinactivation and Repair of Photosystem II. In: Gao, K., Hutchins, D.A., Beardall, J. (eds) Research Methods of Environmental Physiology in Aquatic Sciences. Springer, Singapore. https://doi.org/10.1007/978-981-15-5354-7_24
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DOI: https://doi.org/10.1007/978-981-15-5354-7_24
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