Abstract
Photosynthetic oxygen evolution rate is an important physiological index, which can be used to evaluate the ability of oxygenic organisms to utilize light and inorganic carbon. By using different inhibitors of the photosynthetic electron transport chain and an artificial electron donor/acceptor, the activity of photosynthetic electron transport can also be measured. The oxygen electrode method is the most commonly used method for the measurement of photosynthetic rate. This section will introduce the working principle, operating procedures, matters needing attention, advantages, and disadvantages of the Clark oxygen electrode method for measuring the photosynthetic oxygen evolution rate, by taking several cyanobacterial species as examples.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chen XW, Qiu CE, Shao JZ (2006) Evidence for K+-dependent HCO3- utilization in the marine diatom Phaeodactylum tricornutum. Plant Physiol 141:731–736
Cheng HM, Dai GZ, Yu L et al (2008) Influence of CO2 concentrating mechanism on photoinhibition in Synechococcus sp. PCC7942 (Cyanophyceae). Phycologia 47:588–598
Dai GZ, Deblois CP, Liu SW et al (2008) Differential sensitivity of five cyanobacterial strains to ammonium toxicity and its inhibitory mechanism on the photosynthesis of rice-field cyanobacterium Ge-Xian-Mi (Nostoc). Aquat Toxicol 89:113–121
Gao KS (1996) Research techniques and methods in characterizing photosynthetic carbon fixation by algae. Mar Sci 6:37–41
Gao K, Aruga Y, Asada K et al (1993) Calcification in the articulated coralline alga Corallina pilulifera, with special reference to the effect of elevated CO2 concentration. Mar Biol 117:129–132
Henley WJ (1993) Measurement and interpretation of photosynthetic light-response curves in algae in the context of photoinhibition and diel changes. J Phycol 29:729–739
Qiu BS, Liu JY (2004) Utilization of inorganic carbon in the edible cyanobacterium Ge-Xian-Mi (Nostoc) and its role in alleviating photoinhibition. Plant Cell Environ 27:1447–1458
Zhou WB, Juneau P, Qiu BS (2006) Growth and photosynthetic responses of the bloom-forming cyanobacterium Microcystis aeruginosa to elevated levels of cadmium. Chemosphere 65:1738–1746
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Science Press and Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Dai, G., Mi, H., Qiu, B. (2021). Photosynthetic Oxygen Evolution. In: Gao, K., Hutchins, D.A., Beardall, J. (eds) Research Methods of Environmental Physiology in Aquatic Sciences. Springer, Singapore. https://doi.org/10.1007/978-981-15-5354-7_15
Download citation
DOI: https://doi.org/10.1007/978-981-15-5354-7_15
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-15-5353-0
Online ISBN: 978-981-15-5354-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)