Abstract
Photosynthetic alga, like higher plants, generate reactive oxygen species (ROS), such as superoxide anion radicals (O2−), hydroxyl radicals (·OH) and hydrogen peroxide (H2O2). Normally, the generation and detoxification of ROS in the cell are kept in equilibrium, but the production of ROS could be enhanced by biotic and abiotic stress, in which case algae need mechanisms to protective themselves from oxidative stress. The algal defence system against reactive oxygen involves reactive oxygen scavenging enzymes, including superoxide dismutase (SOD, EC1.15.1.1), catalase (CAT, EC1.11.1.6), peroxidase (POD, EC1.11.1.7), ascorbate peroxidase (APX, EC.1.11.1.11) and glutathione reductase (GR, EC1.6.4.2), and antioxidants such as ascorbate, glutathione, carotenoids, vitamin E, and proline. Here methods are described to determine the SOD, CAT, POD, APX, and GR activities using the marine macroalga Ulva prolifera as experimental material.
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Li, Y., Ma, Z. (2021). Antioxidants and Reactive Oxygen Species (ROS) Scavenging Enzymes. In: Gao, K., Hutchins, D.A., Beardall, J. (eds) Research Methods of Environmental Physiology in Aquatic Sciences. Springer, Singapore. https://doi.org/10.1007/978-981-15-5354-7_10
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DOI: https://doi.org/10.1007/978-981-15-5354-7_10
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