Abstract
Plant-derived drugs represent a significant proportion of the pharmaceutical market. Plant in vitro culture has emerged as a useful platform for producing secondary metabolites due to its multiple advantages: production under controlled culture conditions; independence from geographic, seasonal or weather conditions; and since it avoids the use of land and the extraction from the natural source, it has a low environmental impact. Since the production of secondary metabolites in plant in vitro cultures can be low, there are several strategies that can be applied to enhance the production of these compounds. Elicitation has been widely used to increase secondary metabolite production in plant in vitro cultures. This phenomenon involves the treatment of plant cultures with different agents (physical, chemical or biological) that triggers a defence response, which generally involves the production of secondary metabolites. Anthraquinones are secondary metabolites traditionally used as dyes that exhibit interesting therapeutic applications, such as antiviral (against hepatitis C virus), and as photosensitizers of cancer cells. In this chapter, we will present an approach for the establishment of hairy root cultures of Rubia tinctorum and a protocol for elicitation with methyl jasmonate. We will also discuss the possibility of combining elicitation with other strategies, such as in situ removal with Miglyol® 812.
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Acknowledgements
This work was supported by Agencia Nacional de Promoción Científica y Tecnológica (PICT 2014-3384 and PICT 2014-2118), Universidad de Buenos Aires (UBACyT Q298 2014-2017) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). MP, ABC, VDB, AMG, and JRT are researchers from CONICET. JC is a fellow from CONICET.
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Perassolo, M. et al. (2020). Elicitation as an Essential Strategy for Enhancing Anthraquinone Accumulation in Hairy Root Cultures of Rubia tinctorum. In: Srivastava, V., Mehrotra, S., Mishra, S. (eds) Hairy Root Cultures Based Applications. Rhizosphere Biology. Springer, Singapore. https://doi.org/10.1007/978-981-15-4055-4_10
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