Skip to main content
SpringerLink
Log in

Histopathology Alteration of Red Hybrid Tilapia (Oreochromis Spp.) Caused by Streptococcus Agalactiae Infection

  • Conference paper
  • First Online:
Book cover Charting the Sustainable Future of ASEAN in Science and Technology

  • 254 Accesses

Abstract

Streptococcus agalactiae is one of the streptococcal species most frequently associated with disease in aquaculture. The aim of this study was to assess the histopathology alteration and pathogenicity of S. agalactiae experimentally infected in red hybrid tilapia, Oreochromis spp. Streptococcus agalactiae was isolated from kidney of healthy Oreochromis spp. obtained from wet market. Then, 120 of healthy juveniles of red hybrid tilapia (50–70 g) were injected intraperitoneally with 0.2 ml of S. agalactiae suspension containing 104, 105, 106, 107 and 108 cfu/ml. Fish with clinical signs of streptococcosis were fixed in 10% buffered formalin and proceeded to histopathological examination. As a result, LD50 is 105.8 LD50/ml which is equivalent with bacterial concentration 6.1 × 106. The important histopathology findings were hydropic degeneration of hepatocyte, hypertrophy in liver cells, hemosiderin deposition and haemorrhages on spleen, dilated of Bowman spaces in glomeruli and nephrosis in tubule, and hyperplasia and fusion of secondary lamellae; all these finding were testament for Streptococcosis. In conclusion, this study showed that S. agalactiae isolate was able to produce clinical disease in Red hybrid tilapia, Oreochromis spp. experimentally.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Adikesavalu H, Banerjee S, Patra A, Abraham TJ (2017) Meningoencephalitis in farmed monosex Nile tilapia (Oreochromis niloticus L.) caused by Streptococcus agalactiae. Arch Pol Fish 25(3):187–200

    Google Scholar 

  • Amal MNA, Nur-Nazifah M, Siti-Zahrah A, Sabri MY, Zamri-Saad M (2008) Determination of LD50 for Streptococcus agalactiae infections in red tilapia and GIFT. In: 8th International symposium on tilapia in aquaculture, pp 1245–1251

    Google Scholar 

  • Amal MNA, Zamri-Saad M, Siti-Zahrah A, Zulkafli AR (2013) Transmission of Streptococcus agalactiae from a hatchery into a newly established red hybrid tilapia, Oreochromis niloticus (L.) x Oreochromis mossambicus (Peters), farm. J Fish Dis 1–5

    Google Scholar 

  • Amal MNA, Zamri-Saad M (2011) Streptococcosis in tilapia (Oreochromis niloticus): A Review. Pertanika J Trop Agric Sci 34(2):195–206

    Google Scholar 

  • Anshary H, Kurniawan RA, Sriwulan S, Ramli R, Baxa DV (2014) Isolation and molecular identification of the etiological agents of Streptococcosis in Nile tilapia (Oreochromis niloticus) cultured in net cages in Lake Sentani, Papua, Indonesia. SpringerPlus, 3:627

    Google Scholar 

  • Azad IS, Al-Marzouk A, James CM, Almatar S, Al-Gharabally H, Qasem JA (2012) Outbreak of natural Streptococcosis in hatchery produced silver pomfret (Pampus argenteus Euphrasen) larvae in Kuwait. Aquaculture 330–333:15–20

    Article  Google Scholar 

  • Bernet D, Schmidt H, Meier W, Wahli T (1999) Histopathology in fish: proposal for a protocol to assess aquatic pollution. J Fish Dis 22:25–34

    Article  Google Scholar 

  • Deng ML, Yu ZH, Geng Y, Wang KY, Chen DF, Huang XL et al (2017) Outbreaks of Streptococcosis associated with Streptococcus iniae in Siberian sturgeon (Acipenser baerii) in China. Aquac Res 48(3):909–919

    Article  Google Scholar 

  • Evans JJ, Klesius PH, Gilbert PM, Shoemaker CA, Al Sarawi MA, Landsberg J et al (2002) Characterization of β-haemolytic group B Streptococcus agalactiae in cultured seabream, Sparus auratus L., and wild mullet, Liza klunzingeri (Day), in Kuwait. J Fish Dis 25:505–513

    Article  Google Scholar 

  • Flores-Lopes F, Thomaz AT (2011) Histopathologic alterations observed in fish gills as a tool in environmental monitoring. Braz J Biol 71(1):179–188

    Article  Google Scholar 

  • Garcia JC, Klesius PH, Evans JJ, Shoemaker CA (2008) Non-infectivity of cattle Streptococcus agalactiae in Nile tilapia, Oreochromis niloticus and channel catfish. Ictalurus Punctatus Aquac 281(1–4):151–154

    Article  Google Scholar 

  • Geng Y, Wang KY, Huang XL, Chen DF, Li CW, Ren SY et al (2012) Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya-Fish, Schizothorax prenanti. China Transbound Emerg Dis 59(4):369–375

    Article  Google Scholar 

  • Hernández E, Figueroa J, Iregui C (2009) Streptococcosis on a red tilapia, Oreochromis sp., farm: a case study. J Fish Dis 32(3):247–252

    Google Scholar 

  • Inocente FC, Müller EE, Pretto-Giordano LG, Bracarense APF (2009) Histological findings of experimental Streptococcus agalactiae infection in Nile tilapias (Oreochromis niloticus). Braz J VetY Pathol 2(1):12–15

    Google Scholar 

  • Iregui C, Barato P, Rey A, Vasquez G (2014) Epidemiology of Streptococcus agalactiae and Streptococcosis in tilapia fish. Epidemiology: theory, research & practice, 1st Edition iConcept Press Ltd

    Google Scholar 

  • Jiménez A, Tibatá V, Junca H, Ariza F, Verjan N, Iregui C (2011) Evaluating a nested-PCR assay for detecting Streptococcus agalactiae in red tilapia (Oreochromis sp.) tissue. Aquaculture 321(3):203–206

    Google Scholar 

  • Jantrakajorn S, Maisak H, Wongtavatchai J (2014) Comprehensive investigation of Streptococcosis outbreaks in cultured Nile tilapia, Oreochromis niloticus, and red tilapia, Oreochromis sp., of Thailand. J World Aquac Soc 45(4):392–402

    Google Scholar 

  • Laith AR, Najiah M (2013) Aeromonas hydrophila: Antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). J Aquac Res Dev 5:2

    Google Scholar 

  • Mohamed Fawzy N, Osman KM, Mai El-D, El-S I, Mohamed NMA, Abd-Elrahman SS (2014) Streptococcosis in tilapia: clinico-pathological picture of experimentally infected tilapia. Life Sci J 11(9):1005–1012

    Google Scholar 

  • Ortega YA, Barreiro FS, Bueno HM, Huancaré KP, Ostos HA, Manchego AS et al (2016) First report of Streptococcus agalactiae isolated from Oreochromis niloticus in Piura, Peru: molecular identification and histopathological lesions. Aquac Rep 4:74–79

    Article  Google Scholar 

  • Pretto-Giordano LG, Müller EE, de Freitas JC, da Silva VG (2010) Evaluation on the Pathogenesis of Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). Braz Arch Biol Technol 53(1):87–92

    Article  Google Scholar 

  • Wang K-y, Chen D-f, Huang L-y, Lian H, Wang J, Xiao D et al (2013) Isolation and characterization of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus in China. Afr J Microbiol Res 7(4):317–323

    Article  Google Scholar 

Download references

Acknowledgements

The authors wish to thank Ministry of Malaysian Education for granted Niche Research Grant Scheme Project 5 (R/NRGS/A07.00/00413A/006/000150).

Author information

Authors and Affiliations

  1. Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Kelantan, Malaysia

    Ain Auzureen Mat Zin, Ruhil Hayati Hamdan, Jasni Sabri & Li Peng Tan

  2. Faculty of Agro Based Industry, Universiti Malaysia Kelantan, Jeli, Kelantan, Malaysia

    Mohd Hafiz Jamaludin

Authors
  1. Ain Auzureen Mat Zin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruhil Hayati Hamdan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohd Hafiz Jamaludin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jasni Sabri
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Peng Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ain Auzureen Mat Zin .

Editor information

Editors and Affiliations

  1. Universiti Teknologi MARA Perlis, Perlis, Malaysia

    Nurul Zawani Alias

  2. Universiti Teknologi MARA Perlis, Perlis, Malaysia

    Rizana Yusof

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Singapore Pte Ltd.

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Mat Zin, A., Hamdan, R.H., Jamaludin, M.H., Sabri, J., Tan, L.P. (2020). Histopathology Alteration of Red Hybrid Tilapia (Oreochromis Spp.) Caused by Streptococcus Agalactiae Infection. In: Alias, N., Yusof, R. (eds) Charting the Sustainable Future of ASEAN in Science and Technology . Springer, Singapore. https://doi.org/10.1007/978-981-15-3434-8_19

Download citation

  • DOI: https://doi.org/10.1007/978-981-15-3434-8_19

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-15-3433-1

  • Online ISBN: 978-981-15-3434-8

  • eBook Packages: Social SciencesSocial Sciences (R0)

Publish with us

Policies and ethics

Search

Navigation