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Screening and Characterization of Phosphate-Solubilizing Rhizobia Isolated from Hedysarum pallidum in the Northeast of Morocco

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Phyto-Microbiome in Stress Regulation

Part of the book series: Environmental and Microbial Biotechnology ((EMB))

Abstract

Due to high cost of chemical fertilizers and negative environmental effects, the use of rhizobia as plant growth-promoting rhizobacteria (PGPR) has shown potentials to be a promising technique to assure a sustainable agriculture. Our goal was to select rhizobia strains isolated from nodules of Hydesarum pallidum plants present in Touissit, northeast of Morocco, exhibiting different activities that can stimulate directly and/or indirectly plant growth. A total of 37 bacteria were isolated, of which 19 were capable of solubilizing tricalcium phosphate (TCP). Based on the diameter of solubilization halos (diameter ≥0.4 cm), 15 strains were selected and evaluated for more PGP activities in vitro for selected isolates. As a result, 11 bacteria were proved to be able to synthesize hydrogen cyanide (HCN). Amounts of indole acetic acid (IAA) produced by these bacteria ranged between 1.04 and 3.43 mg L−1. Their ability to secrete siderophores was also evaluated; 80% of the strains were positive for these compounds’ production. We also looked for extracellular enzymes such as cellulase, amylase, protease, chitinase, and urease. The percentages of bacteria that were positive for the production of these hydrolytic enzymes were, respectively, 73.3%, 93.3%, 40%, 26.67%, and 33.3%. Eight of selected bacteria were checked for quantitative assay of TCP solubilization, and soluble P concentrations were between 2 and 137 mg/L, accompanied by a drop in media pH from 5.67 to 3.87. This study reveals the potential of some rhizobia to be used as efficient biofertilizers.

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References

  • Abdul W, Afzal A, Sultan T et al (2014) Isolation and biochemical characterization of rhizobium from pea crop at Swabi. Int J Biosci:231–240

    Google Scholar 

  • Ahemad M, Zaidi A, Khan MS et al (2009) Biological importance of phosphorus and phosphate solubilizing microbes. In: Khan MS, Zaidi A (eds) Phosphate solubilizing microbes for crop improvement. Nova Science Publishers Inc., New York, pp 1–14

    Google Scholar 

  • Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163:173–118

    Article  CAS  Google Scholar 

  • Alikhani HA, Saleh-Rastin N, Antoum H (2006) Phosphate solubilizing activity of rhizobia native to Iranian soils. Plant Soil 287:35–41

    Article  CAS  Google Scholar 

  • Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118

    Article  CAS  Google Scholar 

  • Asghar HN, Zahir ZA, Arshad M, Khaliq A (2002) Relationship between in vitro production of auxins by rhizobacteria and their growth promoting activities in Brassica junceaL. Biol Fertil Soil 35:231–237

    Article  CAS  Google Scholar 

  • Babana AH, Dicko AH, Maïga K, Traoré D (2013) Characterization of rock phosphate-solubilizing microorganisms isolated from wheat (TriticumaestivumL.) rhizosphere in Mali. J Microbiol Microbial Res 1:1–6

    Google Scholar 

  • Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol Biochem 19:451–457

    Article  CAS  Google Scholar 

  • Bhattacharyya P, Jha D (2011) Plant growth-promoting rhizobacteria (PGPsR): emergence in agriculture. World J Microbiol Biotechnol 28:1327–1350

    Article  Google Scholar 

  • Collavino MM, Sansberro PA, Mroginski LA, Aguilar OM (2010) Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth. Biol Fertil Soils 46:727–738

    Article  Google Scholar 

  • Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117

    Article  CAS  Google Scholar 

  • Gordon, Weber RP (1951) Colorimetric estimation of indole-acetic acid. Plant Physiol 26:192–195

    Article  CAS  Google Scholar 

  • Hameeda B, Reddy YHK, Rupela OP, Kumar GN, Reddy G (2006) Effect of carbon substrates on rock phosphate solubilization by bacteria from composts and macrofauna. Curr Microbiol 53:298–302

    Article  CAS  Google Scholar 

  • Hayat R, Ali S, Amara U, Khalid R, Ahmed I (2010) Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol 60:579–598

    Article  Google Scholar 

  • Khan MS, Zaidi A, Wani PA (2007) Role of phosphate-solubilizing microorganisms in sustainable agriculture- a review. Agron Sustain Dev 27:29–43

    Article  Google Scholar 

  • Khan AA, Jilani G, Akhtar MS, Naqvi SMS, Rasheed M (2009a) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1:48–58

    Google Scholar 

  • Khan MS, Zaidi A, Wani PA, Ahemad M, Oves M (2009b) Functional diversity among plant growth-promoting rhizobacteria. In: Khan MS, Zaidi A, Musarrat J (eds) Microbial strategies for crop improvement. Springer, Berlin, pp 105–132

    Chapter  Google Scholar 

  • Kloepper JW, Scher FM, Tripping B (1986) Emergence promoting rhizobacteria: description and implication for agriculture. In: Swinburne TR (ed) Iron, siderophores and plant diseases. Plenum, New York, pp 155–164

    Chapter  Google Scholar 

  • Knowles CJ, Bunch AW (1986) Microbial cyanide metabolism. Adv Microb Physiol 27:73–111

    Article  CAS  Google Scholar 

  • Liu Y, Feng L, H H, Jiang G, Cai Z et al (2012) Phosphorus release from low-grade phosphate rocks by low molecular weight organic acids. J Food Agric Environ 10:1001–1007

    CAS  Google Scholar 

  • Marx JC, Blaise V, Collins T, D’Amico S, Delille D, Gratia E et al (2004) A perspective on cold enzymes: current knowledge and frequently asked questions. Cell Mol Biol (Noisy le-Grand, France) 50(5):643–655

    CAS  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Moataza MS (2006) Destruction of Rhizoctoniasolani and Phytophthoracapsici causing tomato root-rot by Pseudomonas fluorescences lytic enzymes. Res J Agric Biol Sci 2:274–281

    Google Scholar 

  • Naher UA, Radziah O, Shamsuddin ZH, Halimi MS, MRazi I (2009) Isolation of diazotrophs from different soils of tanjongkarang rice growing area in Malaysia. Int J Agric Biol 11:547–552

    CAS  Google Scholar 

  • Pérez E, Sulbaràn M, Ball MM, Yarzàbal LA (2007) Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol Biochem 39:2905–2914

    Article  Google Scholar 

  • Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologica 17:362–370

    CAS  Google Scholar 

  • Renwick A, Campbell R, Coc S (1991) Assesment of in vivo screening systems for potential biocontrol agents of Gaeumannomycesgraminis. Plant Pathol 40:524–532

    Article  Google Scholar 

  • Rodriguez R, Vassilev N, Azcon R (1999) Increases in growth and nutrient uptake of alfalfa grown in soil amended with microbially- treated sugar beet waste. Appl Soil Ecol 11:9–15

    Article  Google Scholar 

  • Sahin F, Cakmakci R, Kantar F (2004) Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant Soil 265:123–129

    Article  CAS  Google Scholar 

  • Schwyn B, Neilands JB (1997) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56

    Article  Google Scholar 

  • Sridevi M, Mallaiah KV (2007) Phosphate solubilization by Rhizobium strains. Indian J Microbiol 49:98–102

    Article  Google Scholar 

  • Tripti, Kumar V, Anshumali (2012) Phosphate solubilizing activity of some bacterial strains isolated from chemical pesticide exposed agricultural soil. Int J Eng Res Dev 3(9):01–06

    Google Scholar 

  • Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586

    Article  CAS  Google Scholar 

  • Vikram A, Hamzehzarghani H (2008) Effect of phosphate solubilizing bacteria on nodulation and growth parameters of greengram (Vigna radiata L. Wilczek). Res J Microbiol 3:62–72

    Article  Google Scholar 

  • Vincent JM (1970) A manual for the practical study of root nodule bacteria. In: IBP Handbook, No 15. Blackwell Scientific Publications Ltd., Oxford

    Google Scholar 

  • Vining LC (1990) Functions of secondary metabolites. Annu Rev Microbiol 44:395–427

    Article  CAS  Google Scholar 

  • Wani PA, Khan MS, Zaidi A (2007) Co-inoculation of nitrogen-fixing and phosphatesolubilizing bacteria to promote growth, yield and nutrient uptake in chickpea. Acta Agron Hung 55:315–323

    Article  CAS  Google Scholar 

  • Wani PA, Khan MS, Zaidi A (2008a) Effect of heavy metal toxicity on growth, symbiosis, seed yield and metal uptake in pea grown in metal amended soil. Bull Environ Contam Toxicol 81:152–158

    Article  CAS  Google Scholar 

  • Wani PA, Khan MS, Zaidi A (2008b) Effect of metal-tolerant plant growth-promoting Rhizobium on the performance of pea grown in metal-amended soil. Arch Environ Contam Toxicol 55:33–42

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Equipe de Recherche de Biotechnologies et Génie des Biomolécules (ERBGB), Faculté des Sciences et Techniques de Tanger, Tanger, Morocco

    Samia Hamane, Anass E. Lyemlahi, Saida Aarab, Amin Laglaoui, Mohammed Bakkali & Abdelhay Arakrak

  2. Faculté polydisciplinaire de Larache, Larache, Morocco

    Mounir Hassani Zerrouk

  3. Faculté des Sciences et Techniquesd’Alhoceima, Ctre Ait Youssef Ou Ali, Morocco

    Saida Aarab

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  1. Samia Hamane
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  2. Mounir Hassani Zerrouk
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  3. Anass E. Lyemlahi
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  4. Saida Aarab
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  5. Amin Laglaoui
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  6. Mohammed Bakkali
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  7. Abdelhay Arakrak
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Editors and Affiliations

  1. Department of Life Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India

    Manoj Kumar

  2. Swami Rama Himalayan University, Himalayan School of Biosciences, Dehradun, Uttarakhand, India

    Vivek Kumar

  3. Department of Botany, Mahatma Gandhi Central University, Motihari, Bihar, India

    Ram Prasad

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Hamane, S. et al. (2020). Screening and Characterization of Phosphate-Solubilizing Rhizobia Isolated from Hedysarum pallidum in the Northeast of Morocco. In: Kumar, M., Kumar, V., Prasad, R. (eds) Phyto-Microbiome in Stress Regulation. Environmental and Microbial Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-15-2576-6_7

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