This review represents the systematic approach to recall the terms, which are used in bioinformatics, such as databases, sequence alignment, docking, and drug discovery. In recent years, the revolution of high sequencing technologies plays a tedious role in the investigation of the bioinformatics. Various analyses of docking and drug discovery were to be discussed. This information helps to know the data which are interrelated between them to precede next. It helps to get an idea to do the experiment with efficient results computationally. This paper helps to know the proper steps to lead the experiments for using various tools, and databases with examples are addressed.