Skip to main content

Development of Standard Protocols for In Vitro Regeneration of Some Selected Banana Cultivars (Musa spp.) from India

  • Chapter
  • First Online:
Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation

Abstract

Banana is a very popular fruit due to its high nutritive value. It helps in reducing the risk of heart diseases and is also recommended for patients suffering from high blood pressure, arthritis, ulcers, and gastrointestinal and kidney disorders. Micropropagation of selected banana cultivars such as Grand Naine (G9), Monthan, and Red Banana on commercial scale using economical cytokinins (BAP) and an effective auxin (IAA) has been taken up in this study in order to supply them to farmers on an affordable price. Consistent shoot proliferation of commercial standard was best in 5 and 3 mg/L BAP for Grand Naine where the production schedule could be formatted accurately with quality shoots. However, for Monthan 10 and 2 mg/L BAP and for Red Banana 10 and 3 mg/L BAP along with 0.2 mg/L IAA for each, respectively, appeared to be more suitable for obtaining productive shoots for a viable commercial production scheduling.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 279.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abdelwahd R, Hakam N, Labhilili M, Udupa M (2008) Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of Faba bean. Afr J Biotechnol 7(8):997–1002

    CAS  Google Scholar 

  • Ahmed S, Sharma A, Singh AK, Wali VK, Kumari P (2014) In vitro multiplication of banana (Musa sp.) cv. Grand Naine. Afr J Biotechnol 13(27):2696–2703

    Article  Google Scholar 

  • Anonymous (2016) Banana—common varieties of banana. http://www.itfnet.org/v1/2016/03/banana-common-varieties/

  • Burrows GE (1989) Developmental anatomy of axillary meristems of Araucaria cunninghamii released from apical dominance following shoot apex decapitation in vitro and in vivo. Univ Chicago Press J 150(4):369–377

    Google Scholar 

  • Dagnew A, Shibru S, Debebe A, Lemma A, Dessalegn L, Berhanu B, Beyene W, Sierra M (2012) Micropropagation of Banana varieties (Musa spp.) using shoot-tip culture. Ethiop J Agric Sci 22:14–25

    Google Scholar 

  • Dayarani M, Dhanarajan MS (2013) Control of excessive browning during in vitro regeneration of Musa laterita. Int J Pharm Bio Sci 4(3):471–476

    CAS  Google Scholar 

  • Fridborg G, Pedersen M, Landstrom L-E, Eriksson T (1978) The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol Plant 43(2):104–106

    Article  CAS  Google Scholar 

  • Ganapathi TR, Suprasanna P, Bapat VA, Rao PS (1992) Propagation of banana through encapsulated shoot tips. Plant Cell Rep 11:571–575

    Article  CAS  PubMed  Google Scholar 

  • Gaspar T, Coumans N (1987) Root formation. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, High-tech and micropropagation 1, vol 2. Springer, Berlin, pp 202–217

    Chapter  Google Scholar 

  • Gubbuk H, Pekmezci M (2004) In vitro propagation of some new banana types (Musa spp.). Turk J Agric For 28:355–361

    Google Scholar 

  • Helaly MN, El-Metwally MA, El-Hoseiny H, Omar SA, El-Sheerys NI (2014) Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana. Aust J Crop Sci 8(4):612–624

    CAS  Google Scholar 

  • Hussein N (2012) Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro. Afr J Biotechnol 11(37)

    Google Scholar 

  • Karule P, Dalvi V, Kadu A, Chaudhari R, Subramaniam VR, Patil AB (2016) A commercial micropropagation protocol for Virupakshi (AAB) banana via apical meristem. Afr J Biotechnol 15(11):401–407

    Article  CAS  Google Scholar 

  • Karuna Y, Rao K (2016) Studies on phenological characters of different banana cultivars (Musa) in Visakhapatnam, Andhra Pradesh. Int J Sci Res (IJSR) 5(5):1689–1693

    Article  Google Scholar 

  • Khatri A, Khan IA, Siddiqui SH, Ahmad M, Siddiqui K (1997) In vitro culture of indigenous and exotic banana clones for maximizing multiplication. Pak J Bot 29:143–150

    Google Scholar 

  • Lalrinsanga R, Vanlaldiki H, Meitei WI (2013) In vitro shoot tip culture of banana cultivar Meitei hei. The Bioscan 8(3):839–844

    Google Scholar 

  • Marín JA, Gella R, Herrero M (1988) Stomatal structure and functioning as a response to environmental changes in acclimatized micropropagated Prunus cerasus L. Ann Bot 62:663–670

    Google Scholar 

  • Mateille T, Foncelle B (1988) Micropropagation of Musa AAA cv. Poyo in the Ivory Coast. Trop Agric (Trinidad) 65:325–328

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth & bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ngomuo M, Mneney E, Ndakdemi PA (2014) The in vitro propagation techniques for producing banana using shoot tip cultures. Am J Plant Sci 5:1614–1622

    Article  Google Scholar 

  • Noor-Aziah J, Khalid N (2002) Comparative analysis of regenerants between single and naked meristem (scalps) of Musa Acuminata Var. Berangan. Asia Pacific J Mol Biol Biotechnol 10:127–131

    Google Scholar 

  • Pan MJ, Van-Staden J (1998) The use of charcoal in in vitro culture—a review. Plant Growth Regul 26:155–163

    Article  CAS  Google Scholar 

  • Pospisilova J, Ticha I, Kadlecek P, Haisel D, Plzakova S (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42(4):48–497

    Google Scholar 

  • Preece JE, Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer Academic Publishers, Dordrecht, pp 71–93

    Chapter  Google Scholar 

  • Priyono (2001) Micropropagation of Banana (Musa paradisiaca) through cormlet initiation by in vitro culture of apical meristem slices. Jurnal Ilmu Dasar 2(1):41–48

    Google Scholar 

  • Rabbani MG, Ali MH, Mondal MF (1996) Effect of BAP and IBA on micropropagation of some banana cultivars. Bangladesh Hort 25(1 & 2):47–52

    Google Scholar 

  • Reddy DDR, Suvarna D, Muralidhra Rao D (2014) Effects of 6-benzyl amino purine (6-BAP) on in vitro shoot multiplication of Grand Naine (Musa sp.). Int J Adv Biotechnol Res 5:36–42

    CAS  Google Scholar 

  • Safwat G, Abdul rahman F, Sharbasy S (2015) The effect of some antioxidants on blackening and growth of in vitro culture of Banana (Musa spp.cv. Grand Naine). Egypt J Genet Cytol 44:47–59

    Google Scholar 

  • Sahoo C, Beura S, Rout S, Beura R (2015) High frequency in vitro cloning of banana (Musa acuminata) cv. Grande Naine. Int J Agric Environ Biotechnol 8(4):943–950

    Google Scholar 

  • Saifuldeen AH (2015) Evaluation of different cytokinins for in vitro multiplication of Banana var. Robusta. Dissertation submitted for the degree of MSc in Botany from Acharya Nagarjuna University and the work carried out at Labland Biotech Private Limited, Mysore

    Google Scholar 

  • Seelye JS, Burge GK, ED Morgan R (2003) Acclimatizing tissue culture plants: reducing the shock. Proc Int Plant Propag Soc 53:85–90

    Google Scholar 

  • Shashikumar R, Krishna V, Ramu V (2015) High frequency plant regeneration of Musa paradisiaca cv. Karibale Monthan. Int J Appl Sci Biotechnol (IJASBT) 3(2):202–209

    Article  CAS  Google Scholar 

  • Strosse H, Houwe I, Panis B, Jain S, Swennen R (2004) Banana cell and tissue culture—review. In: Banana improvement: cellular, molecular biology, and induced mutations. Proceedings of a meeting, Leuven, 2001, pp 1–12

    Google Scholar 

  • Sutter E (1988) Stomatal & cuticular water loss from apple, cherry and sweet gum plants after removal from in vitro culture. J Am Soc Hort Sci 113:234–238

    Google Scholar 

  • Swamy RD, Rao N, Chacko EK (1983) Tissue culture propagation of banana. Sci Hort 18:247–252

    Article  Google Scholar 

  • Thomas T (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631

    Article  CAS  PubMed  Google Scholar 

  • Venkatachalam L, Thimmaraju R, Sreedhar RV, Bhagyalakshmi N (2006) Direct shoot and cormlet regeneration from leaf explants of “silk” banana (AAB). In Vitro Cell Dev Biol Plant 42(3):262–269

    Article  Google Scholar 

  • Vuylsteke DR (1989) Shoot-tip culture for the propagation, conservation and exchange of “Your growing guide to better farming guide”— Manual. Agrilink Series QAL9807

    Google Scholar 

  • Woolley DJ, Wareing PF (1972) The interaction between growth promoters in apical dominance. New Phytol 71:781–793

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Ahmed Hasan, S., Khasim, S.M., Ramudu, J. (2020). Development of Standard Protocols for In Vitro Regeneration of Some Selected Banana Cultivars (Musa spp.) from India. In: Khasim, S.M., Long, C., Thammasiri, K., Lutken, H. (eds) Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation. Springer, Singapore. https://doi.org/10.1007/978-981-15-1636-8_45

Download citation

Publish with us

Policies and ethics