Abstract
High performance liquid chromatography (HPLC) is a form of liquid chromatography which is used to separate the individual components of interest present in mixture and/or dissolved in sample solution. It is based on pumping of mobile phase through the packed column under high pressure. The basic principle involved in HPLC is based on the phenomenon of column chromatography in which the mobile phase is pumped through a packed column by applying high pressure. In this chapter, the different types of HPLC techniques on the basis of mode of chromatography, principle of separation, scale of operation, and the type of analysis have been discussed. The comprehensive instrumentation has also been discussed. At the end of the chapter, advantages and disadvantages along with its applications have been described.
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Further Reading
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Akash, M.S.H., Rehman, K. (2020). High Performance Liquid Chromatography. In: Essentials of Pharmaceutical Analysis. Springer, Singapore. https://doi.org/10.1007/978-981-15-1547-7_14
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DOI: https://doi.org/10.1007/978-981-15-1547-7_14
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