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Biological and Bio-inspired Nanomaterials pp 331–370Cite as

DNA Nanotechnology for Building Sensors, Nanopores and Ion-Channels

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1174))

Abstract

DNA nanotechnology has revolutionised the capabilities to shape and control three-dimensional structures at the nanometre scale. Designer sensors, nanopores and ion-channels built from DNA have great potential for both cross-disciplinary research and applications. Here, we introduce the concept of structural DNA nanotechnology, including DNA origami, and give an overview of the work flow from design to assembly, characterisation and application of DNA-based functional systems. Chemical functionalisation of DNA has opened up pathways to transform static DNA structures into dynamic nanomechanical sensors. We further introduce nanopore sensing as a powerful label-free single-molecule technique and discuss how it can benefit from DNA nanotechnology. Especially exciting is the possibility to create membrane-inserted DNA nanochannels that mimic their protein-based natural counterparts in form and function. In this chapter we review the status quo of DNA sensors, nanopores and ion channels, highlighting opportunities and challenges for their future development.

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Acknowledgements

K.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge and the Oppenheimer PhD studentship, U.F.K. from an ERC Consolidator Grant (Designerpores 647144) and Oxford Nanopore Technologies.

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Authors and Affiliations

  1. Cavendish Laboratory, University of Cambridge, Cambridge, UK

    Kerstin Göpfrich & Ulrich F. Keyser

  2. Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany

    Kerstin Göpfrich

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  1. Kerstin Göpfrich
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  2. Ulrich F. Keyser
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Correspondence to Ulrich F. Keyser .

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  1. National Laboratory of Biomacromolecules, Institute of Biophysics Chinese Academy of Sciences, Beijing, China

    Sarah Perrett

  2. Department of Biotechnology and Biomedicine, Technical University of Denmark DTU, Lyngby, Denmark

    Alexander K. Buell

  3. Department of Chemistry, University of Cambridge, Cambridge, UK

    Tuomas P.J. Knowles

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Göpfrich, K., Keyser, U.F. (2019). DNA Nanotechnology for Building Sensors, Nanopores and Ion-Channels. In: Perrett, S., Buell, A., Knowles, T. (eds) Biological and Bio-inspired Nanomaterials. Advances in Experimental Medicine and Biology, vol 1174. Springer, Singapore. https://doi.org/10.1007/978-981-13-9791-2_11

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