Abstract
The goal of this chapter is to better understand the bioprinting process by theoretically analyzing the correlated topics and questions. It summarizes the general criteria and research route for bioprinting. Despite numerous microextrusion bioprinting strategies developed, very little knowledge has been defined or discovered in terms of the nature of bioprinting. We believe that these strategies share some common features (e.g., one-dimensional filament as building block) that would lead to a better understanding and exploitation of this technology. This chapter will first analyze the general process of microextrusion bioprinting step by step and extract critical questions from each step. The general criteria will be subsequently concluded in terms of structural fidelity and cell protection. Based on the bioink crosslinking mechanisms, such criteria will be used to guide the design of the bioprinting process, covering the filament formation, deposition, and structure stabilization. Overall, a research route will be presented, which will be used in the subsequent case studies. Moreover, this chapter will introduce some general methods used in the study regarding rheology, 3D printability, shear stress determination, and cellular characterization.
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Groll J, Boland T, Blunk T, Burdick JA, Cho DW, Dalton PD, Derby B, Forgacs G, Li Q, Mironov VA, Moroni L, Nakamura M, Shu W, Takeuchi S, Vozzi G, Woodfield TB, Xu T, Yoo JJ, Malda J (2016) Biofabrication: reappraising the definition of an evolving field. Biofabrication 8(1):013001
Blaeser A, Campos DFD, Puster U, Richtering W, Stevens MM, Fischer H (2016) Controlling shear stress in 3D bioprinting is a key factor to balance printing resolution and stem cell integrity. Adv Healthc Mater 5(3):326–333
He Y, Yang F, Zhao H, Gao Q, Xia B, Fu J (2016) Research on the printability of hydrogels in 3D bioprinting. Sci Rep 6:29977
Chung JHY, Naficy S, Yue ZL, Kapsa R, Quigley A, Moulton SE, Wallace GG (2013) Bio-ink properties and printability for extrusion printing living cells. Biomater Sci 1(7):763–773
Kyle S, Jessop ZM, Al-Sabah A, Whitaker IS (2017) ‘Printability’ of candidate biomaterials for extrusion based 3D printing: state-of-the-art. Adv Healthc Mater 6(16)
Radhakrishnan J, Krishnan UM, Sethuraman S (2014) Hydrogel based injectable scaffolds for cardiac tissue regeneration. Biotechnol Adv 32(2):449–461
Malda J, Visser J, Melchels FP, Jungst T, Hennink WE, Dhert WJ, Groll J, Hutmacher DW (2013) 25th anniversary article: engineering hydrogels for biofabrication. Adv Mater 25(36):5011–5028
Colosi C, Shin SR, Manoharan V, Massa S, Costantini M, Barbetta A, Dokmeci MR, Dentini M, Khademhosseini A (2016) Microfluidic bioprinting of heterogeneous 3D tissue constructs using low-viscosity bioink. Adv Mater 28(4):677–684
Yan KC, Nair K, Sun W (2010) Three dimensional multi-scale modelling and analysis of cell damage in cell-encapsulated alginate constructs. J Biomech 43(6):1031–1038
Sung HW, Huang DM, Chang WH, Huang RN, Hsu JC (1999) Evaluation of gelatin hydrogel crosslinked with various crosslinking agents as bioadhesives: in vitro study. J Biomed Mater Res 46(4):520–530
Rowley JA, Madlambayan G, Mooney DJ (1999) Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20(1):45–53
Burdick JA, Prestwich GD (2011) Hyaluronic acid hydrogels for biomedical applications. Adv Mater 23(12):H41–H56
Novosel EC, Kleinhans C, Kluger PJ (2011) Vascularization is the key challenge in tissue engineering. Adv Drug Deliv Rev 63(4–5):300–311
Zhang T, Yan KC, Ouyang L, Sun W (2013) Mechanical characterization of bioprinted soft tissue models. Biofabrication 5(4):045010
Ouyang L, Yao R, Zhao Y, Sun W (2016) Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells. Biofabrication 8(3):035020
Nair K, Gandhi M, Khalil S, Yan KC, Marcolongo M, Barbee K, Sun W (2009) Characterization of cell viability during bioprinting processes. Biotechnol J 4(8):1168–1177
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© 2019 Tsinghua University Press, Beijing and Springer Nature Singapore Pte Ltd.
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Ouyang, L. (2019). Materials and Methods. In: Study on Microextrusion-based 3D Bioprinting and Bioink Crosslinking Mechanisms. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-9455-3_3
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DOI: https://doi.org/10.1007/978-981-13-9455-3_3
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