Abstract
The microorganism play an essential role in various metabolic activity associated with health, obesity, immune system, complex carbohydrate, nitrogen, sulfur, and xenobiotic metabolism etc. The identification of microorganism involved in such process is becoming possible with the sequencing of 16S rRNA amplicon and responsible gene through molecular cloning and then sequencing. The first-generation sequencing extensively facilitated the molecular characterization of microorganism and functional gene with expense of high cost with low throughput. The advent of next-generation sequencing technology enables the high-scale full-length 16S rRNA molecular characterization and genome sequencing with reduced time and cost with high yield. The present article describes available genomes in public database and the role of next- and third-generation sequencing technology contribution to the growth of genome and metagenome sequencing and its associated projects, their taxonomy, and functional characterization through bioinformatic analysis. This chapter also provides an overview on the metagenomic sequencing and functional characterization of three important ecological niches, viz., rumen, soil, and human gut. The massive advancement in high-throughput sequencing technology and bioinformatic analysis enabled robust genome and metagenome characterization in short time with reduced budget.
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Reddy, B. (2019). Advancements in Microbial Genome Sequencing and Microbial Community Characterization. In: Tripathi, V., Kumar, P., Tripathi, P., Kishore, A. (eds) Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore. https://doi.org/10.1007/978-981-13-8739-5_5
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