Toxicokinetics of Nano Materials After the Intratracheal Administration
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Toxicokinetic data is important to evaluate the toxicity of nano materials because burden and toxicity for each organ can be different among particles even at same burden. Intratracheal administration has been proposed to use as screening method for the evaluation of inhalation toxicity to nano materials. In the manuscript, toxicokinetic test methods, deposition/clearance/distribution, and toxicokinetic models of nano materials after intratracheal administration mainly conducted in METI Project are described. By using XRF and ICP-MS, the deposition, clearance, and distribution were determined in the animal tests. Insoluble and poorly soluble nano particles detected in lung were 44–100% at 1–3 days after intratracheally administration, while poorly soluble coarse particle, which is likely to be trapped at bronchiole, detected in lung at 1–3 days after intratracheally administration were only 5–40%. Twenty-eight days after the intratracheal administration, the lung contained <1% (soluble NiO), 37–90% (poorly soluble NiO and Al(OH)3-coated TiO2), 40–62% (insoluble TiO2 particles), 11–30% (amorphous SiO2), and 21–63% (crystal SiO2) of administered doses. Translocation from lung to extrapulmonary organs was quite low except for lymph nodes.
KeywordsAnimal test ICP-MS XRF Deposition Clearance Distribution Compartment model
This work is part of the research program “Development of innovative methodology for safety assessment of industrial nanomaterials,” supported by the Ministry of Economy, Trade and Industry (METI) of Japan.
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