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Taurine Ameliorates High Glucose Induced Apoptosis in HT-22 Cells

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Taurine 11

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1155))

Abstract

Diabetes causes memory loss. Hippocampus is responsible for memory and increased apoptosis was found in diabetes patients. Taurine improved memory in diabetes condition. However, mechanism is unclear. In current study, hippocampal cell line HT-22 cells were subjected to analysis as five groups i.e. Control, High glucose (HG) at concentration of 150 mM, HG + 10 mM (T1), 20 mM (T2) and 40 mM (T3) taurine solution. TUNEL assay showed that HG increased the number of apoptotic cell significantly while taurine reduced apoptosis. Taurine increased phosphorylation of Akt in HT-22 cell treated with HG, and increased phosphorylation of Bad (p-Bad) was seen suggesting involvement of Akt/Bad signaling pathway. Expression of Bcl-2 was reduced in HG group but taurine improved this. Bax expression showed opposite trend. This indicated that taurine may reduce apoptosis by controlling balance of Bcl-2 and Bax. When the activation of Akt was blocked by using of perifosine, the effect of taurine disappears either partially or altogether. Thus, it was clear that taurine reduces apoptosis via Akt/Bad pathway in HT-22 cells exposed to HG which further improves downstream balance of Bcl-2 and Bax. This mechanism may be involved in apoptosis of hippocampus cells in diabetic condition.

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Change history

  • 12 October 2019

    Affiliations of authors Muhammad Shahbaz and Shahid Alam were incorrect in the published book. This has now been corrected as below:

Abbreviations

DM:

Diabetes mellitus

MTT:

4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide

HG:

High glucose

TUNEL:

In situ TdT-mediated dUTP nick end labeling

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Acknowledgement

This work was supported by National Natural Science Foundation of China (grant numbers 81501574).

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Author notes

  1. Pingan Wu and Xiaochi Chen as co-first author contributed equally to this work.

Authors and Affiliations

  1. Department of Occupational and Environmental Health, Dalian Medical University, Dalian, China

    Pingan Wu,  Inam-u-llah, Xiaoxia Shi, Mengren Zhang, Kaixin Li & Fengyuan Piao

  2. Department of Urology, The First Affiliated Hospital of Dalian Medical University, Dalian, China

    Xiaochi Chen

  3. Institute of Food Science and Technology, Graduate School of Chinese Academy of Agriculture Science, Beijing, China

    Raheel Suleman

  4. Department of Food Science and Technology, Muhammad Nawaz Sharif University of Agriculture, Multan, Pakistan

    Muhammad Shahbaz

  5. Department of Anatomy, Dalian Medical University, Dalian, China

    Shahid Alam

Authors
  1. Pingan Wu
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  2. Xiaochi Chen
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  3. Inam-u-llah
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  4. Xiaoxia Shi
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  5. Mengren Zhang
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  6. Kaixin Li
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  7. Raheel Suleman
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  8. Muhammad Shahbaz
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  9. Shahid Alam
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  10. Fengyuan Piao
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Editor information

Editors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China

    Jianmin Hu

  2. Department of Occupational and Environmental Health, Dalian Medicine University, Dalian, Liaoning, China

    Fengyuan Piao

  3. Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA

    Stephen W. Schaffer

  4. Department of Biology, Center for Developmental Neuroscience, College of Staten Island, Staten Island, NY, USA

    Abdeslem El Idrissi

  5. Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA

    Jang-Yen Wu

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© 2019 Springer Nature Singapore Pte Ltd.

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Wu, P. et al. (2019). Taurine Ameliorates High Glucose Induced Apoptosis in HT-22 Cells. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_75

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