Role of Rhizobacteria in Drought Tolerance

  • Meghmala Waghmode
  • Aparna Gunjal
  • Neha Patil
  • Neelu Nawani
Part of the Microorganisms for Sustainability book series (MICRO, volume 12)


Drought is the most destructive abiotic stress affecting the world’s food security. Rhizospheric and endophytic bacteria produce range of enzymes and metabolites, which help the plants to tolerate abiotic stress. Induced systemic resistance gets developed in plants surviving in drought conditions. Drought tolerance is induced in crops due to the production of exopolysaccharides, phytohormones like gibberellic acid, cytokinins, abscisic acid, and IAA, ACC deaminase, antioxidants, osmolytes, and volatile compounds. Plants in drought conditions survive due to rhizobacteria enhancing photosynthetic activity. PGPR improves the growth, antioxidant activity, and photosynthetic activity of the crops in drought conditions. Rhizobacteria assist in resource attainment, i.e., nitrogen, phosphorus, and essential minerals by changing the root morphology, improving the soil structure, and bioremediation of the polluted soils.


Exopolysaccharides Phytohormones Antioxidant Indole-3-acetic acid Bioremediation 


  1. Alexieva V, Sergiev I, Mapelli S, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344CrossRefGoogle Scholar
  2. Arkhipova T, Prinsen E, Veselov S, Martinenko E, Melentiev A, Kudoyarova G (2007) Cytokinin producing bacteria enhance plant growth in drying soil. Plant Soil 292:305–315CrossRefGoogle Scholar
  3. Aroca R, Ferrante A, Vernieri P, Chrispeels M (2006) Drought, abscisic acid and transpiration rate effects on the regulation of PIP aquaporin gene expression and abundance in Phaseolus vulgaris plants. Ann Bot 98:1301–1310PubMedPubMedCentralCrossRefGoogle Scholar
  4. Arshad M, Sharoona B, Mahmood T (2008) Inoculation with Pseudomonas spp. containing ACC deaminase partially eliminate the effects of drought stress on growth, yield, and ripening of pea (P. sativum L.). Pedosphere 18:611–620CrossRefGoogle Scholar
  5. Blum A (2005) Drought resistance, water-use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Aust J Agric Res 56:1159–1168CrossRefGoogle Scholar
  6. Bottner P, Couteaux M, Vallejo R (1995) Soil organic matter in Mediterranean-type ecosystems and global climatic changes: a case study-the soils of the Mediterranean basin. In: Jose M, Oechel C (eds) Global change and Mediterranean-type ecosystems ecological studies. Springer, New York, pp 306–325CrossRefGoogle Scholar
  7. Bresson J, Vasseur F, Dauzat M, Labadie M, Varoquax F, Touraine B, Vile D (2014) Interact to survive: Phyllobacterium brassicacearum improves Arabidopsis tolerance to severe water deficit and growth recovery. PLoS One 9:e107607PubMedPubMedCentralCrossRefGoogle Scholar
  8. Casanovas M, Barassi A, Sueldo J (2002) Azospirillum inoculation mitigate water stress effects in maize seedlings. Cereal Res Commun 30:343–350Google Scholar
  9. Cohen A, Bottini R, Pontin M, Berli F, Moreno D, Boccanlandro H, Travaglia C, Picocoli P (2015) Azospirillum brasilense ameliorates the response of Arabidopsis thaliana to drought mainly via enhancement of ABA levels. Physiol Plant 153:79–90CrossRefGoogle Scholar
  10. Creus M, Graziano M, Casanovas M, Pereyra A, Simontacchi M, Puntarulo S, Barassi A, Lamattina L (2005) Nitric oxide is involved in the Azospirillum brasilense induced lateral root formation in tomato. Planta 221:297–303PubMedCrossRefGoogle Scholar
  11. Egamberdieva D, Kucharova Z (2009) Selection for root-colonizing bacteria stimulating wheat growth in saline soils. Biol Fertil Soil 45:561–573CrossRefGoogle Scholar
  12. Farooq M, Wahid A, Kobayashi N, Fujita D, Basra A (2009) Plant drought stress: effects, mechanisms, and management. Agron Sustain Dev 29:185–212CrossRefGoogle Scholar
  13. German A, Burdman S, Okon Y, Kigel J (2000) Effects of Azospirillum brasilense on root morphology of common bean (Phaseolus vulgaris L.) under different water regimes. Biol Fertil Soil 32:259–264CrossRefGoogle Scholar
  14. Glick B (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117CrossRefGoogle Scholar
  15. Gowda P, Henry A, Yamauchi A, Shashidhar E, Serraj R (2011) Root biology and genetic improvement for drought avoidance in rice. Field Crops Res 122:1–13CrossRefGoogle Scholar
  16. Grover M, Madhubala R, Ali Z, Yadav K, Venkateswarlu B (2014) Influence of Bacillus spp. strains on seedling growth and physiological parameters of sorghum under moisture stress conditions. J Basic Microbiol 54:951–961CrossRefGoogle Scholar
  17. Guo Z, Ou W, Lu S, Zhong Q (2006) Differential responses of the antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem 44:828–836PubMedCrossRefGoogle Scholar
  18. Gururani A, Upadhyaya P, Baskar V, Venkatesh J, Nookaraju A, Park W (2013) Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS-Scavenging enzymes and improved photosynthetic performance. J Plant Growth Regul 32:245–258CrossRefGoogle Scholar
  19. Hardoim R, Van Overbeek S, Van Elsas D (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 16:463–471PubMedCrossRefGoogle Scholar
  20. Helena M, Carvalho C (2008) Drought stress and reactive oxygen species production, scavenging and signaling. Plant Signal Behav 3:156–165CrossRefGoogle Scholar
  21. Hepper M (1975) Extracellular polysaccharides of soil bacteria. In: Walker N (ed) Soil microbiology, a critical review. Wiley, New York, pp 93–111Google Scholar
  22. Hsiao A (2000) Effect of water deficit on morphological and physiological characterizes in rice (Oryza sativa). J Agric For 3:93–97Google Scholar
  23. Huang B, DaCosta M, Jiang Y (2014) Research advances in mechanisms of turfgrass tolerance to abiotic stresses: from physiology to molecular biology. Crit Rev Plant Sci 33:141–189CrossRefGoogle Scholar
  24. Jaleel A, Manivannan P, Wahid A, Farooq M, Al-Juburi J, Somasundaram R, Vam P (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. Int J Agric Biol 11:100–105Google Scholar
  25. Kamara Y, Menkir A, Badu-Apraku B, Ibikunle O (2003) The influence of drought stress on growth, yield and yield components of selected maize genotypes. J Agric Sci 141:43–50CrossRefGoogle Scholar
  26. Kasim A, Osman E, Omar N, Abd El-Daim A, Bejai S, Meijer J (2013) Control of drought stress in wheat using plant growth promoting bacteria. J Plant Growth Regul 32:122–130CrossRefGoogle Scholar
  27. Kiani P, Talia P, Maury P, Grieu P, Heinz R, Perrault A, Nishinakamasu V, Hopp E, Gentzbittel L, Paniego N, Sarrafi A (2007) Genetic analysis of plant water status and osmotic adjustment in recombinant inbred lines of sunflower under two water treatments. Plant Sci 172:773–787CrossRefGoogle Scholar
  28. Lafitte R, Yongsheng G, Yan S, Lil K (2007) Whole plant responses, key processes, and adaptation to drought stress: the case of rice. J Exp Bot 58:169–175PubMedCrossRefGoogle Scholar
  29. Lesk C, Rowhani P, Ramankutty N (2016) Influence of extreme weather disasters on global crop production. Nature 529:84–87CrossRefGoogle Scholar
  30. Lum S, Hanafi M, Rafii M, Akmar N (2014) Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. J Anim Plant Sci 24:1487–1493Google Scholar
  31. Mafakheri A, Siosemardeh A, Bahramnejad B, Struik C, Sohrabi Y (2010) Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust J Crop Sci 4:580–585Google Scholar
  32. Marulanda A, Barea M, Azcón R (2009) Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. J Plant Growth Regul 28:115–124CrossRefGoogle Scholar
  33. Mayak S, Tirosh T, Glick R (2004) Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci 166:525–530CrossRefGoogle Scholar
  34. Naseem H, Bano A (2014) Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance in maize. J Plant Interact 9:689–701CrossRefGoogle Scholar
  35. Nilsen T, Orcutt M (1996) The physiology of plants under stress. Wiley, New YorkGoogle Scholar
  36. Rahdari P, Hoseini M, Tavakoli S (2012) The studying effect of drought stress on germination, proline, sugar, lipid, protein and chlorophyll content in Purslane (Portulaca oleracea L.) leaves. J Med Plant Res 6:1539–1547Google Scholar
  37. Rampino P, Pataleo S, Gerardi C, Perotta C (2006) Drought stress responses in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell Environ 29:2143–2152PubMedCrossRefGoogle Scholar
  38. Rejeb I, Pastor V, Mauch-Mani B (2014) Plant responses to simultaneous biotic and abiotic stress: molecular mechanisms. Plan Theory 3:458–475Google Scholar
  39. Samarah H (2005) Effects of drought stress on growth and yield of barley. Agron Sustain Dev 25:145–149CrossRefGoogle Scholar
  40. Sankar B, Jaleel A, Manivannan P, Kishorekumar A, Somasundaram R, Panneerselvam R (2007) Drought-induced biochemical modifications and proline metabolism in Abelmoschus esculentus (L) Moench. Acta Bot Croat 61:43–56Google Scholar
  41. Saravanakumar D, Kavino M, Raguchander T, Subbian P, Samiyappan R (2011) Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiol Plant 33:203–209CrossRefGoogle Scholar
  42. Sarma R, Saikia R (2014) Alleviation of drought stress in mung bean by strain Pseudomonas aeruginosa GGRJ21. Plant Soil 377:111–126CrossRefGoogle Scholar
  43. Selvakumar G, Panneerselvam P, Ganeshamurthy N (2012) Bacterial mediated alleviation of abiotic stress in crops. In: Maheshwari K (ed) Bacteria in agrobiology: stress management. Springer, Berlin/Heidelberg, pp 205–224CrossRefGoogle Scholar
  44. Serraj R, Sinclair R (2002) Osmolyte accumulation: can it really help increase crop yield under drought condition? Plant Cell Environ 25:331–341CrossRefGoogle Scholar
  45. Shakir A, Asghari B, Arshad M (2012) Rhizosphere bacteria containing ACC deaminase conferred drought tolerance in wheat grown under semi-arid climate. Soil Environ 31:108–112Google Scholar
  46. Silvente S, Sobolev P, Lara M (2012) Metabolite adjustment in drought tolerant and sensitive genotypes in response to water stress. PLoS One 7:e38554PubMedPubMedCentralCrossRefGoogle Scholar
  47. Sudhakar P, Kumar K, Latha P, Sruthi S, Sujatha K, Reddy B, Reddy R, Rajareddy K, Krishna G, Reddy S (2013) Recent advances in biofertilizers and biofungicides (PGPR) for sustainable agriculture. In: Reddy S, Ilao I, Faylon S, Dar D, Sayyed R, Sudini H, Kumar K, Armada A (eds) Proceeding of 3rd Asian Conference on plant growth promoting rhizobacteria (PGPR) and other microbes. Manila, Philippines, pp 268–274Google Scholar
  48. Timmusk S, Grantcharova N, Wagner G (2005) Paenibacillus polymyxa invades plant roots and forms biofilms. Appl Environ Microbiol 71:7292–7300PubMedPubMedCentralCrossRefGoogle Scholar
  49. Timmusk S, Abd El-Daim IA, Lucian C, Tanilas T, Kannaste A, Behers L, Nevo E, Seisenbaeva G, Stenstrom E, Niinemets U (2014) Drought-tolerance of wheat improved by rhizosphere bacteria from harsh environments: enhanced biomass production and reduced emissions of stress volatiles. PLoS One 9:1–13CrossRefGoogle Scholar
  50. Vilchez I, Garcia-Fontana C, Roman-Naranjo D, Gonzalez-Lopez J, Manzanera M (2016) Plant drought tolerance enhancement by trehalose production of desiccation-tolerant microorganisms. Front Microbiol 7:1577PubMedPubMedCentralCrossRefGoogle Scholar
  51. Wahid A, Gelani S, Ashraf M, Foolad R (2007) Heat tolerance in plants: an overview. Environ Exp Biol 61:199–223Google Scholar
  52. Wilkinson S, Davies J (2010) Drought, ozone, ABA and ethylene: new insights from cell to plant to the community. Plant Cell Environ 33:510–525PubMedCrossRefGoogle Scholar
  53. Zhou Y, Lambrides C, Kearns R, Ye C, Fukai S (2012) Water use, water use efficiency and drought resistance among warm-season turfgrasses in shallow soil profiles. Funct Plant Biol 39:116–125CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Meghmala Waghmode
    • 1
  • Aparna Gunjal
    • 2
  • Neha Patil
    • 1
  • Neelu Nawani
    • 3
  1. 1.Department of MicrobiologyAnnasaheb Magar MahavidyalayaHadapsar, PuneIndia
  2. 2.Department of Environmental ScienceHaribhai V. Desai CollegePuneIndia
  3. 3.Dr. D. Y. Patil Vidyapeeth’s Dr. D. Y. Patil Biotechnology & Bioinformatics InstituteTathawade, PuneIndia

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