Skip to main content
SpringerLink
Log in
Book cover

Current Aspects of Flavonoids: Their Role in Cancer Treatment pp 9–22Cite as

Analytical Techniques for the Identification and Quantification of Flavonoids

  • Chapter
  • First Online:

Abstract

Flavonoids are of great interest in relation to their actions in living organisms and their role in biological and physiological interactions. In regard to the significance of the pharmaceutical nature of flavonoids, the focus at present is on rapid, cheap, reproducible, and precise analytical procedures to measure flavonoids in a variety of matrices. These procedures are necessary for quality control, for the standardization of extracts, for determining the number of flavonoids, and for determining the pharmacokinetic properties of flavonoids in humans. This chapter outlines various analytical procedures, such as liquid chromatography, gas chromatography, capillary electrophoresis, and thin-layer chromatography, for the investigation of key flavonoids. Because of the growing interest in flavonoid structure, the focus will be on the use of tandem-mass spectrometric techniques for the characterization of some significant flavonoid subclasses, and on the possibility of using combined diode-array detector ultraviolet and nuclear magnetic resonance for unambiguous detection.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Avula B, Wang YH, Rumalla CS, Smillie TJ, Khan IA (2012) Simultaneous determination of alkaloids and flavonoids from aerial parts of Passiflora species and dietary supplements using UPLC-UV-MS and HPTLC. Nat Prod Commun 7(9):1177–1180

    CAS  Google Scholar 

  • Baghel US, Nagar A, Pannu MS, Singh D, Yadav R (2017) HPLC and HPTLC methods for simultaneous estimation of quercetin and curcumin in polyherbalformulation. Indian J Pharm Sci 79(2):197–203

    Article  Google Scholar 

  • Berthod A, Ruiz-Angel MJ, Carda-Broch S (2009) Countercurrent chromatography: people and applications. J Chromatogr A 1216(19):4206–4217

    Article  CAS  Google Scholar 

  • Bhandari P, Kumar N, Gupta AP, Singh B, Kaul VK (2007) A rapid RP-HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants. J Sep Sci 30(13):2092–2096

    Article  CAS  Google Scholar 

  • Bhatt V, Sharma S, Kumar N, Sharma U, Singh B (2017) Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS. J Pharm Biomed Anal 132:46–55

    Article  CAS  Google Scholar 

  • Bilu V, Male Ž, Golja P, Cetina-Čižmek B (2005) HPTLC determination of flavonoids and phenolic acids in some Croatian Stachys taxa. J Planar Chromatogr 18(104):269–273

    Article  Google Scholar 

  • Braunberger C, Zehl M, Conrad J, Fischer S, Adhami HR, Beifuss U, Krenn L (2013) LC-NMR, NMR, and LC-MS identification and LC-DAD quantification of flavonoids and ellagic acid derivatives in Droserapeltata. J Chromatogr B 932:111–116

    Article  CAS  Google Scholar 

  • Canini A, Alesiani D, D’Arcangelo G, Tagliatesta P (2007) Gas chromatography-mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf. J Food Compos Anal 20(7):584–590

    Article  CAS  Google Scholar 

  • Chaudhary A, Sharma U, Vig AP, Singh B, Arora S (2014) Free radical scavenging, antiproliferative activities and profiling of variations in the level of phytochemicals in different parts of broccoli (Brassica oleracea italica). Food Chem 148:373–380

    Article  CAS  Google Scholar 

  • Chaudhary A, Choudhary S, Sharma U, Vig AP, Singh B, Arora S (2018) Purple head broccoli (Brassica oleracea L. var. italicaPlenck), a functional food crop for antioxidant and anticancer potential. J Food SciTechnol 55(5):1806–1815

    CAS  Google Scholar 

  • Chen LJ, Games DE, Jones J (2003) Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography. J Chromatogr A 988(1):95–105

    Article  CAS  Google Scholar 

  • Chen LJ, Song H, Games DE, Sutherland IA (2005) HSCCC-MS study of flavonoids in the extracts from the seeds of Oroxylum indicum. J Liq Chromatogr Relat Technol 28(12–13):1993–2003

    Article  CAS  Google Scholar 

  • Chen XJ, Ji H, Wang YT, Li SP (2008) Simultaneous determination of seven flavonoids in Epimedium using pressurized liquid extraction and capillary electrochromatography. J Sep Sci 31(5):881–887

    Article  CAS  Google Scholar 

  • Cui J, Yue Y, Tang F, Wang J (2011) HPTLC analysis of the flavonoids in eight species of Indocalamus leaves. J Planar Chromatogr Mod TLC 24(5):394–399

    Article  CAS  Google Scholar 

  • Duan K, Yuan Z, Guo W, Meng Y, Cui Y, Kong D, Zhang L, Wang N (2011) LC-MS/MS determination and pharmacokinetic study of five flavone components after solvent extraction/acid hydrolysis in rat plasma after oral administration of Verbena officinalis L. extract. J Ethnopharmacol 135(2):201–208

    Article  CAS  Google Scholar 

  • Engida AM, Kasim NS, Tsigie YA, Ismadji S, Huynh LH, Ju YH (2013) Extraction, identification and quantitative HPLC analysis of flavonoids from sarangsemut (Myrmecodiapendan). Ind Crop Prod 41:392–396

    Article  CAS  Google Scholar 

  • Fabre N, Rustan I, de Hoffmann E, Quetin-Leclercq J (2001) Determination of flavone, flavonol, and flavanoneaglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry. J Am Soc Mass Spectrom 12(6):707–715

    Article  CAS  Google Scholar 

  • Farag MA, Huhman DV, Lei Z, Sumner LW (2007) Metabolic profiling and systematic identification of flavonoids and isoflavonoids in roots and cell suspension cultures of Medicago truncatula using HPLC–UV–ESI–MS and GC–MS. Phytochemistry 68(3):342–354

    Article  CAS  Google Scholar 

  • Fonseca FN, Tavares MF, Horváth C (2007) Capillary electrochromatography of selected phenolic compounds of Chamomilla recutita. J Chromatogr A 1154(1–2):390–399

    Article  CAS  Google Scholar 

  • Frison-Norrie S, Sporns P (2002) Identification and quantification of flavonol glycosides in almond seedcoats using MALDI-TOF MS. J Agric Food Chem 50(10):2782–2787

    Article  CAS  Google Scholar 

  • Füzfai Z, Molnár-Perl I (2007) Gas chromatographic-mass spectrometric fragmentation study of flavonoids as their trimethylsilyl derivatives: analysis of flavonoids, sugars, carboxylic and amino acids in model systems and in citrus fruits. J Chromatogr A 1149(1):88–101

    Article  Google Scholar 

  • Gonnella NC (2013) LC-NMR: expanding the limits of structure elucidation. CRC Press, Boca Raton

    Book  Google Scholar 

  • He D, Huang Y, Ayupbek A, Gu D, Yang Y, Aisa HA, Ito Y (2010) Separation and purification of flavonoids from blackcurrant leaves by high-speed countercurrent chromatography and preparative HPLC. J Liq Chromatogr Relat Technol 33(5):615–628

    Article  CAS  Google Scholar 

  • Hostettmann K, Terreaux C (2000) Medium pressure liquid chromatography. In: Wilson ID (Chief ed) Encyclopedia of separation science. Academic, San Diego, pp 3296–3303

    Chapter  Google Scholar 

  • Huang Y, Feng Y, Tang G, Li M, Zhang T, Fillet M, Crommen J, Jiang Z (2017) Development and validation of a fast SFC method for the analysis of flavonoids in plant extracts. J Pharm Biomed Anal 140:384–391

    Article  CAS  Google Scholar 

  • Lafont R, Dauphin-Villemant C, Warren JT, Rees H (2012) Ecdysteroid chemistry and biochemistry. In: Gilbert LI (ed) Insect endocrinology. Academic, London, pp 106–176

    Chapter  Google Scholar 

  • Lee JS, Kim DH, Liu KH, Oh TK, Lee CH (2005) Identification of flavonoids using liquid chromatography with electrospray ionization and ion trap tandem mass spectrometry with an MS/MS library. Rapid Commun Mass Spectrom 19(23):3539–3548

    Article  CAS  Google Scholar 

  • Lee YS, Kim SH, Kim JK, Lee S, Jung SH, Lim SS (2011) Preparative isolation and purification of seven isoflavones from Belamcanda chinensis. Phytochem Anal 22(5):468–473

    Article  CAS  Google Scholar 

  • Li J, Zhang X, Yu Q, Fu X, Wang W (2014) One-step separation of four flavonoids from Herba Salviae Plbeiae by HSCCC. J ChromatogrSci 52(10):1288–1293

    Article  CAS  Google Scholar 

  • Madaan R, Bansal G, Kumar S, Sharma A (2011) Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian J Pharm Sci 73(6):666

    Article  CAS  Google Scholar 

  • Marston A (2007) Role of advances in chromatographic techniques in phytochemistry. Phytochemistry 68(22–24):2786–2798

    Article  CAS  Google Scholar 

  • Mattila P, Astola J, Kumpulainen J (2000) Determination of flavonoids in plant material by HPLC with diode-array and electro-array detections. J Agric Food Chem 48(12):5834–5841

    Article  CAS  Google Scholar 

  • Monagas M, Quintanilla-López JE, Gómez-Cordovés C, Bartolomé B, Lebrón-Aguilar R (2010) MALDI-TOF MS analysis of plant proanthocyanidins. J Pharm Biomed Anal 51(2):358–372

    Article  CAS  Google Scholar 

  • Oliveira RN, Mancini MC, Oliveira FCSD, Passos TM, Quilty B, Thiré RMDSM, McGuinness GB (2016) FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing. Matéria (Rio de Janeiro) 21(3):767–779

    Article  CAS  Google Scholar 

  • Pereira CA, Yariwake JH, Lanças FM, Wauters JN, Tits M, Angenot L (2004) A HPTLC densitometric determination of flavonoids from Passiflora alata, P. edulis,P. incarnata and P. caerulea and comparison with HPLC method. Phytochem Anal 15(4):241–248

    Article  CAS  Google Scholar 

  • Sarker SD, Nahar L (2012) Hyphenated techniques and their applications in natural products analysis. In: Sarker S, Nahar L (eds) Natural products isolation. Methods in molecular biology (Methods and protocols), vol 864. Humana Press, New York, pp 301–340

    Chapter  Google Scholar 

  • Schmidt TJ, Merfort I, Willuhn G (1994) Gas chromatography-mass spectrometry of flavonoid aglycones II. Structure-retention relationships and a possibility of differentiation between isomeric 6-and 8-methoxyflavones. J Chromatogr A 669(1–2):236–240

    Article  CAS  Google Scholar 

  • Segura-Carretero A, Puertas-Mejía MA, Cortacero-Ramírez S, Beltrán R, Alonso-Villaverde C, Joven J, Dinelli G, Fernández-Gutiérrez A (2008) Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight/ion trap). Electrophoresis 29(13):2852–2861

    Article  CAS  Google Scholar 

  • Sharma V, Chaudhary A, Arora S, Saxena AK, Ishar MPS (2013) β-Ionone derived chalcones as potent antiproliferative agents. Eur J Med Chem 69:310–315

    Article  CAS  Google Scholar 

  • Sharma V, Chaudhry A, Chashoo G, Arora R, Arora S, Ishar MPS (2014) β-Ionone derived apoptosis inducing endoperoxides; discovery of potent leads for anticancer agents. Eur J Med Chem 87:228–236

    Article  CAS  Google Scholar 

  • Sulaiman CT, Balachandran I (2016) LC/MS characterization of antioxidant flavonoids from Tragia involucrata L. Beni-Suef Univ J Basic Appl Sci 5(3):231–235

    Article  Google Scholar 

  • Sun Y, Fang N, Chen DD, Donkor KK (2008) Determination of potentially anti-carcinogenic flavonoids in wines by micellar electrokinetic chromatography. Food Chem 106(1):415–420

    Article  CAS  Google Scholar 

  • Wang X, Liang Y, Zhu L, Xie H, Li H, He J, Pan M, Zhang T, Ito Y (2010) Preparative isolation and purification of flavone C-glycosides from the leaves of Ficus microcarpa Lf by medium-pressure liquid chromatography, high-speed countercurrent chromatography, and preparative liquid chromatography. J Liq Chromatogr Relat Technol 33(4):462–480

    Article  Google Scholar 

  • Weisz GM, Kammerer DR, Carle R (2009) Identification and quantification of phenolic compounds from sunflower (Helianthus annuus L.) kernels and shells by HPLC-DAD/ESI-MSn. Food Chem 115(2):758–765

    Article  CAS  Google Scholar 

  • Wu J, Fang XA, Yuan Y, Dong Y, Liang Y, Xie Q, Ban J, Chen Y, Lv Z (2017) UPLC/Q-TOF-MS profiling of phenolics from Canarium pimela leaves and its vasorelaxant and antioxidant activities. Rev BrasFarmacogn 27(6):716–723

    CAS  Google Scholar 

  • Wulandari L, Retnaningtyas Y, Lukman H (2016) Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics. J Anal Methods Chem 2016:1–6

    Article  Google Scholar 

  • Zehl M, Braunberger C, Conrad J, Crnogorac M, Krasteva S, Vogler B, Beifuss U, Krenn L (2011) Identification and quantification of flavonoids and ellagic acid derivatives in therapeutically important Drosera species by LC–DAD, LC–NMR, NMR, and LC–MS. Anal BioanalChem 400(8):2565–2576

    Article  CAS  Google Scholar 

  • Zhang S, Dong S, Chi L, He P, Wang Q, Fang Y (2008) Simultaneous determination of flavonoids in chrysanthemum by capillary zone electrophoresis with running buffer modifiers. Talanta 76(4):780–784

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, M. M. Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, India

    Ashun Chaudhary

  2. Faculty of Biology, Technion-Israel Institute of Technology, Technion City-Haifa, Israel

    Praveen Kumar

  3. Department of Chemistry, M. M. Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Mullana-Ambala, India

    Vivek Sheel Jaswal

  4. PG Department of Zoology, DAV College, Jalandhar, Punjab, India

    Abhinay Thakur

Authors
  1. Ashun Chaudhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Praveen Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vivek Sheel Jaswal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abhinay Thakur
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana, India

    Hardeep Singh Tuli

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Chaudhary, A., Kumar, P., Jaswal, V.S., Thakur, A. (2019). Analytical Techniques for the Identification and Quantification of Flavonoids. In: Singh Tuli, H. (eds) Current Aspects of Flavonoids: Their Role in Cancer Treatment . Springer, Singapore. https://doi.org/10.1007/978-981-13-5874-6_2

Download citation

Publish with us

Policies and ethics

Search

Navigation