Investigating Dielectric Properties of Hemolysis by Electrical Impedance Spectroscopy

Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 69)


This paper presents a study toward the investigation of electrical properties of static bovine blood samples during hemolysis by electrochemical impedance spectroscopy (EIS). RBC hemolysis was induced by adding different volume fractions of distilled water into static bovine blood samples that causes cell membrane breakage and release of cytoplasm into blood plasma. EIS measurements were conducted with frequency range between f = 100 kHz and f = 300 MHz. Our data show that changes in electrical properties indicating hemolysis are observed under high frequency conditions. Moreover, the impedance increase related to the release of cytoplasm into plasma and increment of hemoglobin’s amount. The highest resistance of second semi-circle (Z′) shows a linear relationship with the hemoglobin concentration (Hb). This relationship is described by the equation Z′ = 0.7932Hb + 15.788, with correlation coefficient of 0.9978.


Hemolysis Electrical impedance spectroscopy Red blood cells 



The author wishes to acknowledge the financial support by JSPS KAKENHI grant Number JP16H02312.

Conflict of Interest The authors declare that they have no conflict of interest.


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© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Graduate School of Mechanical Engineering, Division of Artificial System ScienceChiba UniversityChibaJapan
  2. 2.Department of Biomedical EngineeringInternational University, Vietnam National University-Ho Chi Minh City (VNU-HCM)Thu Duc District, Ho Chi Minh CityVietnam

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