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Decellularization of Bovine Cancellous Bone for Bone Tissue Engineering Application

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7th International Conference on the Development of Biomedical Engineering in Vietnam (BME7) (BME 2018)


Cancellous bovine bone represents an inexhaustible source of material for bone tissue engineering. However, its natural marrow-filled structure can greatly limit the efficacy and prolong the duration of the decellularization procedure. Here this research formulates an efficient, shorten decellularization protocol for cancellous bovine bone with an initial delipidation step to improve the effect of following methods. The samples were then treated with a series of physical and chemical treatments including thermal shock and detergent wash. The samples acquired from this protocol have shown effective removal of cellular materials and fat while maintaining the essential bone extracellular matrix, which was confirmed through scanning electron microscopy observation and histological staining results. The decellularized bone showed potential to be further investigated, developed and combined with other material to create biocompatible scaffold for bone tissue engineering application.

Nam Minh-phuong Tran and Dat Tan Nguyen—Contributed equally.

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  1. Thi Hiep, N., Chan Khon, H., Dai Hai, N., Byong-Taek, L., Van Toi, V., Thanh Hung, L.: Biocompatibility of PCL/PLGA-BCP porous scaffold for bone tissue engineering applications. J. Biomater. Sci. Polym. Ed. 28(9), 864–878 (2017)

    Article  Google Scholar 

  2. Nguyen, T.-H., Bao, T.Q., Park, I., Lee, B.-T.: A novel fibrous scaffold composed of electrospun porous poly (ɛ-caprolactone) fibers for bone tissue engineering. J. Biomater. Appl. 28(4), 514–528 (2013)

    Article  Google Scholar 

  3. Campana, V., Milano, G., Pagano, E., Barba, M., Cicione, C., Salonna, G., Lattanzi, W., Logroscino, G.: Bone substitutes in orthopaedic surgery: from basic science to clinical practice. J. Mater. Sci. Mater. Med. 25(10), 2445–2461 (2014)

    Article  Google Scholar 

  4. Nguyen, T.H., Lee, B.T.: In vitro and in vivo studies of rhBMP2-coated PS/PCL fibrous scaffolds for bone regeneration. J. Biomed. Mater. Res. Part A 101(3), 797–808 (2013)

    Article  Google Scholar 

  5. Zhang, N., Zhou, M., Zhang, Y., Wang, X., Ma, S., Dong, L., Yang, T., Ma, L., Li, B.: Porcine bone grafts defatted by lipase: efficacy of defatting and assessment of cytocompatibility. Cell Tissue Banking 15(3), 357–367 (2014)

    Article  Google Scholar 

  6. Khan, S.N., Cammisa Jr., F.P., Sandhu, H.S., Diwan, A.D., Girardi, F.P., Lane, J.M.: The biology of bone grafting. JAAOS-J. Am. Acad. Orthop. Surg. 13(1), 77–86 (2005)

    Article  Google Scholar 

  7. Elsalanty, M.E., Genecov, D.G.: Bone grafts in craniofacial surgery. Craniomaxillofacial Trauma Reconstr. 2(03), 125–134 (2009)

    Article  Google Scholar 

  8. Katz, J., Mukherjee, N., Cobb, R.R., Bursac, P., York-Ely, A.: Incorporation and immunogenicity of cleaned bovine bone in a sheep model. J. Biomater. Appl. 24(2), 159–174 (2009)

    Article  Google Scholar 

  9. Badylak, S.F., Taylor, D., Uygun, K.: Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu. Rev. Biomed. Eng. 13, 27–53 (2011)

    Article  Google Scholar 

  10. Chappard, D., Fressonnet, C., Genty, C., Baslé, M.-F., Rebel, A.: Fat in bone xenografts: importance of the purification procedures on cleanliness, wettability and biocompatibility. Biomaterials 14(7), 507–512 (1993)

    Article  Google Scholar 

  11. Moreau, M.F., Gallois, Y., Baslé, M.-F., Chappard, D.: Gamma irradiation of human bone allografts alters medullary lipids and releases toxic compounds for osteoblast-like cells. Biomaterials 21(4), 369–376 (2000)

    Article  Google Scholar 

  12. Gardin, C., Ricci, S., Ferroni, L., Guazzo, R., Sbricoli, L., De Benedictis, G., Finotti, L., Isola, M., Bressan, E., Zavan, B.: Decellularization and delipidation protocols of bovine bone and pericardium for bone grafting and guided bone regeneration procedures. PLoS ONE 10(7), e0132344 (2015).

    Article  Google Scholar 

  13. Anderson, J.M., Rodriguez, A., Chang, D.T.: Foreign body reaction to biomaterials. In: Seminars in Immunology, vol. 2, pp. 86–100. Elsevier (2008)

    Google Scholar 

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This research is funded by International University, Vietnam National University Ho Chi Minh City under grant number: 03-BME-2017/HĐSV-QLKH

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The authors declare that they have no conflict of interest.

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Correspondence to Nam Minh-phuong Tran .

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Tran, N.Mp., Nguyen, D.T., Luong, T.D., Bui, N.H., Van Toi, V., Nguyen, TH. (2020). Decellularization of Bovine Cancellous Bone for Bone Tissue Engineering Application. In: Van Toi , V., Le, T., Ngo, H., Nguyen, TH. (eds) 7th International Conference on the Development of Biomedical Engineering in Vietnam (BME7). BME 2018. IFMBE Proceedings, vol 69. Springer, Singapore.

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